Land cover samples are usually the foundation for supervised classification. Unfortunately, for land cover mapping in large areas, only limited samples can be used due to the time-consuming and labor-intensive sample collection. A novel and practical Object-oriented Iterative Classification method based on Multiple Classifiers Ensemble (OIC-MCE) was proposed in this paper. It systematically integrated object-oriented segmentation, Multiple Classifier Ensemble (MCE), and Iterative Classification (IC). In this method, the initial training samples were updated self-adaptively during the iterative processes. Based on these updated training samples, the inconsistent regions (ICR) in the classification results of the MCE method were reclassified to reduce their uncertainty. Three typical case studies in the China-Pakistan Economic Corridor (CPEC) indicate that the overall accuracy of the OIC-MCE method is significantly higher than that of the single classifier. After five iterations, the overall accuracy of the OIC-MCE approach increased by 5.58%-8.38% compared to the accuracy of the traditional MCE method. The spatial distribution of newly added training samples generated by the OIC-MCE approach was relatively uniform. It was confirmed by ten repeated experiments that the OIC-MCE approach has good stability. More importantly, even if the initial sample size reduced by 65%, the quality of the final classification result based on the proposed OIC-MCE approach would not be greatly affected. Therefore, the proposed OIC-MCE approach provides a new solution for land cover mapping with limited samples. Certainly, it is also well suited for land cover mapping with abundant samples.Remote Sens. 2020, 12, 987 2 of 21 various homogeneous or heterogeneous data sources, advanced machine learning algorithms, and a variety of sampling and classification strategies have been introduced into land cover mapping researches [1,[5][6][7].Supervised classification is the most commonly used method in land cover mapping studies, especially for generating a regional or even a global land cover product [8]. Samples are the foundation of this method. The quantity and quality of samples would have a significant impact on the accuracy of land cover products [9,10]. There are many methods to collect samples, such as field survey, crowdsourcing, and manual interpretation [11]. In general, field survey has been a simple and practical method, but it cannot be conducted in inaccessible regions [12]. More importantly, it is time-consuming and labor-intensive. Manual interpretation of high-resolution remote sensing images, relying on the interpretation experiences of the interpreters, has been an effective way to fill the blank of field surveys. However, the subjectivity of the interpreter might affect the objectivity of samples [10]. Crowdsourcing was a new technology that helps to collect samples [13,14]. Unfortunately, it is still in the experimental stage at present, with few available samples and a lack of objective evaluation for their reliability...