Today it is difficult not to notice the influence of human beings and the reduced area of the natural environment: forests are turned into agricultural areas, agricultural land into suburban construction, suburban areas into urban areas. Vineyard landscapes form a unique image of the region, contributing to their uniqueness and identity (they represent cultural heritage, function as trademarks for their areas, attract visitors, inspire art, serve as places for different, spiritual activities). In the chapter, the authors analyze the eco factors that have the greatest influence in the wine-growing area of Serbia: climatic and anthropogeographical. Using the method of comparative analysis, GIS techniques, and cartographic visualization, results were obtained that show that the most environmental changes in Serbia's wine destinations were due to anthropographic factors: land cover use and the least due to climatic factors, although the analysis period is much longer for climatic factors.