Supplemental description of <i>Paraphelenchus acontioides</i> (Tylenchida&#x3A; Aphelenchidae, Paraphelenchinae), with ribosomal DNA trees and a morphometric compendium of female <i>Paraphelenchus</i>

Abstract

Nematodes were isolated from surface-sterilised stems of cheatgrass, Bromus tectorum (Poaceae), in Colorado, grown on Fusarium (Hypocreaceae) fungus culture, and identified as Paraphelenchus acontioides. Morphometrics and micrographic morphology of this species are given to supplement the original description and expand the comparative species diagnosis. A tabular morphometric compendium of the females of the 23 species of Paraphelenchus is provided as the last diagnostic compilation was in 1984. Variations in the oviduct within the genus are reviewed to evaluate the taxonomic assignment of P. deckeri, a morphologically transitional species between Aphelenchus and Paraphelenchus. Sequences were generated for both 18S and 28S ribosomal DNA, representing the first identified species within Paraphelenchus so characterised. These sequences were incorporated into phylogenetic trees with related species of Aphelenchidae and Tylenchidae. Aphelenchus avenae isolates formed a well supported monophyletic sister group to Paraphelenchus. The ecology of Paraphelenchus, cheat grass and Fusarium is also discussed.

Preliminary survey of nematodes associated with broadleaved trees in İzmit Forest Management Directorate, Turkey

“…In our study, phylogenetic analysis was not run for P. acontioides because it is a nominal species and does not have enough isolates to be compared. The genus Paraphelenchus Micoletzky, 1922 harbouring more than 20 species including P. acontioides is generally considered to be mycetophagous (Hunt, 1993) and mostly associated with plants (Carta et al, 2011). Paraphelenchus acontioides was recovered and described from soil around the roots of Kentucky bluegrass (Agrotis palustris Huds.)…”

Morphological and Molecular Characterization of Two Aphelenchoides Endophytic in Poplar Leaves

“…These include fungal-feeding Paraphelenchus acontioides Taylor and Pillai, 1967 (Carta et al, 2011) and bacterial-feeding Panagrolaimus artyukhovskii Blinova andMishina, 1975 (Baynes et al, 2012) within cheatgrass stems from Colorado. Two other species of fungal-feeding nematodes were isolated from within dicots, specifically from the leaves of two species of poplar (P. angustifolia and P. trichocarpa) originating in Utah and western Washington, respectively.…”