Supplementary Data and Methods from Expansion of Tumor-Infiltrating CD8⁺ T cells Expressing PD-1 Improves the Efficacy of Adoptive T-cell Therapy

Abstract

<p>Supplementary Figures: (Fig. S1) Multi-parametric flow cytometric analysis of tumor-infiltrating T lymphocyte subsets from freshly excised MC38 tumors; (Fig. S2) Expression of co-inhibitory and co-stimulatory molecules over the course of tumor progression; (Fig. S3) Tumor-reactivity of CD137+ and PD-1+ TILs, freshly after isolation from tumors and after in vitro expansion; (Fig. S4) Kinetic of expansion of different CD8 TIL subsets isolated from MC38 tumors; (Fig. S5) CDR3 spectratype analysis. Supplementary Tables: (Table S1) Number of CDR3 fragments which dominance hierarchy changed after TIL expansion; (Table S2) Number of TCR-Vbeta families that lose, preserve or acquire new dominant CDR3 fragments after TIL expansion. Supplemental Material and Methods: Spectratyping.</p>