Supplementary Figures S1-10 from Genome-wide RNAi Screening Identifies RFC4 as a Factor That Mediates Radioresistance in Colorectal Cancer by Facilitating Nonhomologous End Joining Repair

Abstract

<p>Supplementary Figure S1 shows the procedure to generate radioresistant HCT1166GyR cells; Supplementary Figure S2 shows reporter constructs of NHEJ and HR pathway; Supplementary Figure S3 shows representative images of IHC assays; Supplementary Figure S4 show the detailed data of screening candidate radioresistant genes; Supplementary Figure S5 shows RFC4 expression in CRC cells and tissues after radiation; Supplementary Figure S6 shows the representative clonogenic pictures for Figure 1C; Supplementary Figure S7 shows representative charts of Annexin V/PI double staining assays for Figure 1F; Supplementary Figure S8 shows the effects of RFC4 on γ-H2AX and pATM levels after radiation; Supplementary Figure S9 shows the additional evidences of RFC4 to promote NHEJ- but not HR-mediate DNA repair; Supplementary Figure S10 shows the effects of RFC4 on the chemotherapeutic drug resistance in HCT116 cells.</p>