Supplementary reinoculation in topdressing of<i>Rhizobium tropici</i>in common bean crop: effects on nodulation, morphology, and grain yield

Sousa, Westefann dos Santos; Teixeira, Itamar Rosa; Campos, Thiago Souza; Silva, Gisele Carneiro da; Silva, Maryelle Barros da; Moreira, Silvino Guimarães

doi:10.1080/01904167.2021.2020833

Cited by 5 publications

(7 citation statements)

References 22 publications

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“…Pérola of 3.339 kg ha −1 in a treatment that involved inoculation with rhizobia via seed (strain SEMIA 4077), equivalent to the application of 80 kg ha −1 of N and therefore close to the average productivity obtained in this study. Similarly, in a study carried out by Sousa et al [40] with beans cv. BRS Valente, a productivity of 3.976 kg ha −1 was obtained for the treatment that involved cover inoculation + supplementary cover reinoculation carried out at the V4 stage, corroborating the results expressed in this work and confirming the efficiency of the inoculation process in the context of beans when combined with seed inoculation and cover reinoculation carried out at the V4 stage.…”

Section: Discussionmentioning

confidence: 72%

Potential of Reinoculation Using R. tropici Applied under Cover to Increase Bean Yield

Lourenço,

Teixeira,

Rocha

et al. 2023

Sustainability

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The common bean is known to require nitrogen, with mineral fertilizer being the main form of supply to the plants. However, more efficient inoculants containing strains of Rhizobium tropici that are potentially more efficient in fixing atmospheric N2 could replace the use of nitrogen fertilizers as long as some of the existing challenges in the application methodology are overcome (for example, ensuring the existence of active nodules in the most demanding crop phases: flowering and grain filling). This study aimed to verify the responses of common bean plants subjected to reinoculation using Rhizobium tropici in topdressing applied at different stages in a greenhouse and in a field between March and April 2020 and in the spring–summer harvest of 2020/2021, respectively. A randomized block design was used, along with four replications: T1 = uninoculated control; T2 = inoculation via seed (VS); T3 = VS + reinoculation at the V4 stage; T4 = VS + reinoculation at the R5 stage; T5 = VS + reinoculation at the R6 stage; T6 = VS + reinoculation at the V4 and R5 stages; T7 = VS + reinoculation at the V4 and R6 stages; T8 = VS + reinoculation at the R5 and R6 stages; T9 = VS + reinoculation at the V4, R5, and R6 stages; and T10 = mineral nitrogen fertilization. Nodulation and morphological variables were evaluated at the R7 stage, and agronomic variables were evaluated at the R9 stage at harvest. The use of reinoculation, in addition to inoculation, positively influenced the bean nodulation process. The morphological characteristics of the plants showed good development with the supply of N (carried out via inoculation and reinoculation), equivalent to the development that could be achieved via the addition of a mineral nitrogen fertilizer. Reinoculation from the R5 to R7 stages proved ineffective in supplying N to the common bean crop. Inoculation in the seed associated with reinoculation in coverage at the V4 stage provided higher yields (3.271 kg ha−1), reinforcing the potential for mineral nitrogen fertilizers to be replaced. The use of the seed inoculation technique with subsequent reinoculation under cover can completely replace the use of nitrogenous mineral fertilizers in fertilizing bean plants, making the cultivation of this fabacea more sustainable in terms of the associated costs and environmental problems.

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Section: Discussionmentioning

confidence: 72%

Potential of Reinoculation Using R. tropici Applied under Cover to Increase Bean Yield

Lourenço,

Teixeira,

Rocha

et al. 2023

Sustainability

Self Cite

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“…Darker seed-coat color is reported to contain greater levels of flavonoids, anthocyanins, and anthocyanidins that promote superior symbiotic functioning [ 17 , 19 ]. Hungria and Phillips [ 17 ] and dos Santos Sousa [ 20 ] have, in fact, shown that common bean ( Phaseolus vulgaris L.) seedlings generated from a black-seeded genotype formed more root nodules compared with its white isogenic counterpart.…”

Section: A Historical Perspective On Legume Seed-coat Pigmentationmentioning

confidence: 99%

Seed-Coat Pigmentation Plays a Crucial Role in Partner Selection and N2 Fixation in Legume-Root–Microbe Associations in African Soils

Jaiswal,

Dakora

2024

Plants

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Legume–rhizobia symbiosis is the most important plant–microbe interaction in sustainable agriculture due to its ability to provide much needed N in cropping systems. This interaction is mediated by the mutual recognition of signaling molecules from the two partners, namely legumes and rhizobia. In legumes, these molecules are in the form of flavonoids and anthocyanins, which are responsible for the pigmentation of plant organs, such as seeds, flowers, fruits, and even leaves. Seed-coat pigmentation in legumes is a dominant factor influencing gene expression relating to N2 fixation and may be responsible for the different N2-fixing abilities observed among legume genotypes under field conditions in African soils. Common bean, cowpea, Kersting’s groundnut, and Bambara groundnut landraces with black seed-coat color are reported to release higher concentrations of nod-gene-inducing flavonoids and anthocyanins compared with the Red and Cream landraces. Black seed-coat pigmentation is considered a biomarker for enhanced nodulation and N2 fixation in legumes. Cowpea, Bambara groundnut, and Kersting’s bean with differing seed-coat colors are known to attract different soil rhizobia based on PCR-RFLP analysis of bacterial DNA. Even when seeds of the same legume with diverse seed-coat colors were planted together in one hole, the nodulating bradyrhizobia clustered differently in the PCR-RFLP dendrogram. Kersting’s groundnut, Bambara groundnut, and cowpea with differing seed-coat colors were selectively nodulated by different bradyrhizobial species. The 16S rRNA amplicon sequencing also found significant selective influences of seed-coat pigmentation on microbial community structure in the rhizosphere of five Kersting’s groundnut landraces. Seed-coat color therefore plays a dominant role in the selection of the bacterial partner in the legume–rhizobia symbiosis.

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“…The importance of seed coat color in legume nodulation has been properly documented [15,17,[19][20][21], and darker seed coat color is reported to contain greater levels of flavonoids, anthocyanins, and anthocyanidins that promote superior symbiotic functioning [17,22,23]. Hungria and Phillips [17] and dos Santos Sousa [24] have, in fact, shown that common bean (Phaseolus vulgaris L.) seedlings generated from a black-seeded genotype formed more root nodules compared to its white isogenic counterpart.…”

Section: A Historical Perspective On Legume Seed Coat Pigmentationmentioning

confidence: 99%

Seed Coat Pigmentation Plays a Crucial Role in Partner Selection and N2 Fixation in Legume Root-Microbe Associations in African Soils

Jaiswal,

Dakora

2024

Preprint

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The legume-rhizobia symbiosis is one of the most important plant-microbe interactions in sustainable agriculture due to its ability to provide much needed N to cropping systems. This interaction is mediated by the mutual recognition of signaling molecules from the two partners, namely legumes and rhizobia. With legumes, these molecules are in the form of flavonoids and anthocyanins, which are responsible for the pigmentation of plants parts, such as seeds, flowers, fruits and even leaves. Seed coat pigmentation in legumes is a dominant factor influencing gene expression relating to N2 fixation, and may be responsible for the different N2-fixing abilities observed among legume genotypes under field conditions in African soils. Cowpea, Kersting’s bean and Bambara groundnut landraces with black seed coat color are reported to release higher concentrations of nod-gene-inducing flavonoids and anthocyanins when compared to the Red and Cream landraces, hence the black seed coat pigmentation is considered a biomarker for enhanced nodulation and N2 fixation. Cowpea, Bambara groundnut and Kersting’s bean with differing seed coat colors are known to attract different native soil rhizobia, confirmable by PCR-RFLP analysis of bacterial DNA from root nodules of these legumes. Even when seeds of the same legume with diverse seed coat colors were planted together in one hole, the nodulating bradyrhizobia clustered differently in the PCR-RFLP dendrograms. In one study, Kersting’s groundnut, Bambara groundnut and cowpea with differing seed coat colors were selectively nodulated by different bradyrhizobial species. Multilocus sequence analysis showed that different Bradyrhizobium species nodulated the Kersting's bean based on seed coat color. Phylogenetic analysis also placed the bradyrhizobial isolates in close proximity to different Bradyrhizobium species such as B. vignae 7-2T, B. subterraneum 58 2-1T, B. kavangense 14-3T, B. liaoningense 2281 (USDA 3622)T, B. yuanmingense LMG 21827T, B. huanghuaihaiense CCBAU 23303T, B. pachyrhizi PAC48T, and a reference type strain of B. elkanii according to seed coat color. Using 16S rDNA amplicon sequencing, we also found significant selective influences of seed coat pigmentation on microbial community structure in the rhizosphere of five Kersting’s groundnut landraces. For example, the rhizosphere of Belane Mottled landrace was dominated by Proteobacteria, while Bacteroidetes dominated the rhizospheres of the other landraces. With legumes, seed coat pigmentation therefore plays a dominant role in the selection of the bacterial symbiotic partner.

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Supplementary reinoculation in topdressing ofRhizobium tropiciin common bean crop: effects on nodulation, morphology, and grain yield

Cited by 5 publications

References 22 publications

Potential of Reinoculation Using R. tropici Applied under Cover to Increase Bean Yield

Potential of Reinoculation Using R. tropici Applied under Cover to Increase Bean Yield

Seed-Coat Pigmentation Plays a Crucial Role in Partner Selection and N2 Fixation in Legume-Root–Microbe Associations in African Soils

Seed Coat Pigmentation Plays a Crucial Role in Partner Selection and N2 Fixation in Legume Root-Microbe Associations in African Soils

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