Background: Lambs or sheep are considered an excellent animal model for humans owing to advantages related to bone anatomy, formation, biomechanical characteristics; bone strength; and absorption of minerals and vitamins. Moreover, bone healing in many animal species is faster than that in humans, whereas bone healing, turnover, and remodelling in sheep and humans are comparable. In this context, it would be interesting to examine the effects of bioactive components of diet (including Se and carnosic acid) on bone mineralisation and strength, since these are the most important indicators of bone status. This knowledge may be useful in the context of orthopaedic research, recovery after orthopaedic surgeries, and prevention of skeletal diseases in humans.Results: The aim of the present study was to assess the effects partial replacement of rapeseed oil (RO) with fish oil (FO) combined with dietary supplementation of various antioxidants on the characteristics of lamb femur. Thirty male lambs were assigned to five dietary treatments and fed isoproteinous and isoenergetic diets for 35 days. The control diet was enriched with 3.0% RO, while the experimental diets were enriched either only with 2.0% RO and 1.0% FO or additionally with 0.1% carnosic acid, 0.1% carnosic acid and 0.35 ppm Se as selenised yeast, or 0.1% carnosic acid and 0.35 ppm Se as sodium selenite. After 35 days, the lambs were slaughtered, and the femur was dissected from the carcass of each animal and analysed for morphometric properties.Conclusions: The present study indicated that dietary bioactive components may improve bone health by promoting bone mineralisation in lambs. Partial replacement of RO with FO combined with dietary supplementation of carnosic acid and organic Se improved the geometric, densitometric, and biomechanical properties of lamb femur.