Supplementation of Bacillus subtilis and Lactobacillus casei to increase growth performance and immune system of catfish (Clarias gariepinus) due to Aeromonas hydrophila infection

Aini, Nurul; Putri, Dini Sarafina Yulia Rosa; Achhlam, Divany Hunaimatul; Fatimah, Fatimah; Andriyono, Sapto; Hariani, Dyah; Do, Hoang Dang Khoa; Wahyuningsih, Sri Puji Astuti

doi:10.14202/vetworld.2024.602-611

Cited by 2 publications

(1 citation statement)

References 65 publications

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“…Probiotics have been investigated for their ability to improve growth performance and overall health in African catfish (Genç et al, 2020). Some studies reported that supplementation dietary with probiotics can increase SGR of African catfish (Aini et al, 2024;Mansour et al, 2024). Furthermore, the use of probiotics was found to improve feed utilization, body indices, and protein efficiency rate in African catfish (Lawal et al, 2019) Bacteria collected from aquatic and land animal intestines are commonly used as probiotics in aquaculture.…”

Section: Specific Growth Ratementioning

confidence: 99%

DIETARY PROBIOTICS AND ITS EFFECT ON GROWTH RATE, SURVIVAL RATE, AND FEED CONVERSION RATIO OF Clarias gariepinus

Hadijah,

Loar,

Mardiana

et al. 2024

Jurnal Riset Akuakultur

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The applications of probiotics in aquaculture show wide range of potential benefits. If fish consume probiotics in sufficient and accurate amounts, it can have a beneficial impact on the health of catfish. The study aimed to identify the effect of dietary probiotics supplementation of artificial diet at different doses on the African catfish (Clarias gariepinus) parameters such as specific growth rate, survival rate, and feed conversion ratio. The experiment was performed at the Faculty of Agriculture, Bosowa University, Makassar, and lasted for 2 months. The experiment employed a completely randomized design (CRD) consisting of four treatments and three replicates. The EM4 probiotics were selected as the probiotic supplement and divided into four different doses including treatment: A (5 mL per 100 g feed), B (10 mL per 100g feed), C (15 mL per 100g feed), and D (control). A total of 120 African catfish fingerlings were fed twice every 07:00 a.m. and 06:00 p.m. with a feeding rate of 5% of the body weight. Sampling was performed every week to record catfish body weight and length. The analysis of variance indicated a significant effect of dietary EM4 probiotics supplementation on specific growth rate and feed conversion ratio of African cathfish. However, the result of the experiment revealed 15 mL per 100 g feed did not promote absolute growth rate and survival rate significantly. This study concluded that supplementation of probiotics at a dose of 15 mL per 100 g feed in diet could improve the growth of catfish.Penerapan probiotik dalam budidaya perikanan menunjukkan berbagai manfaat potensial. Jika ikan mengonsumsi probiotik dalam jumlah yang cukup dan tepat, maka dapat memberikan dampak yang menguntungkan bagi kesehatan ikan lele dumbo. Penelitian ini bertujuan untuk mengidentifikasi pengaruh pemberian probiotik pada pakan buatan dengan dosis berbeda terhadap laju pertumbuhan spesifik, sintasan, dan rasio konversi pakan ikan lele dumbo (Clarias gariepinus). Eksperimen dilakukan di Fakultas Pertanian, Universitas Bosowa, Makassar, dan berlangsung selama 2 bulan. Eksperimen menggunakan rancangan acak lengkap (RAL) yang terdiri dari empat perlakuan dan tiga ulangan. Probiotik EM4 dipilih sebagai suplemen probiotik dan dibagi menjadi empat dosis perlakuan yang berbeda meliputi perlakuan A (5 mL per 100 g pakan), perlakuan B (10 mL per 100 g pakan), perlakuan C (15 mL per 100 g pakan), dan perlakuan D (kontrol). Sebanyak 120 ekor benih ikan lele dumbo diberi pakan dua kali setiap pukul 07.00 dan 18.00 dengan dosis pemberian pakan sebanyak 5% dari bobot tubuh ikan lele. Pengambilan sampel dilakukan setiap minggu untuk mencatat bobot dan panjang tubuh ikan lele. Analisis varians menunjukkan pengaruh yang signifikan dari suplementasi probiotik EM4 pada laju pertumbuhan spesifik dan rasio konversi pakan ikan lele dumbo. Namun, hasil percobaan menunjukkan bahwa 15 mL per 100 g pakan tidak meningkatkan laju pertumbuhan mutlak dan sintasan secara signifikan. Penelitian ini menyimpulkan bahwa suplementasi probiotik 15 mL per 100 g pakan dalam pakan dapat meningkatkan pertumbuhan ikan lele dumbo.

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Section: Specific Growth Ratementioning

confidence: 99%

DIETARY PROBIOTICS AND ITS EFFECT ON GROWTH RATE, SURVIVAL RATE, AND FEED CONVERSION RATIO OF Clarias gariepinus

Hadijah,

Loar,

Mardiana

et al. 2024

Jurnal Riset Akuakultur

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Potential Probiotic Bacillus Strains with Antioxidant and Antimutagenic Activity Increased Weight Gain and Altered hsp70, cxc, tnfα, il1β, and lysC Gene Expression in Clarias gariepinus

Skripnichenko,

Chelombitskaya,

Prazdnova

et al. 2024

Fishes

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The potential probiotic properties of three Bacillus strains were studied. A probiotic supplement for the African catfish Clarias gariepinus was produced via the solid-state fermentation protocol and incorporated into the fish feed for a period of seven weeks. Since the 36th day of the experiment, all experimental groups had a statistically significant increase in their weight gain than the control group. The maximum weight gain observed in fish fed the probiotic-supplemented feed was 29.16% higher than that of the control group, and the maximum feed conversion rate improvement was 24%. Cell-free extracts from these strains showed antioxidant (11.55–27.40%) and DNA-protective (45.33–61.83%) activity in a series of in vitro biosensor tests. Further investigation into the antimutagenic activity of the strains revealed that two of them reduced the level of induced mutagenesis in an Escherichia coli model (by 33.58% and 54.35%, respectively). We also assessed the impact of probiotic strains on the expression of several key genes in the host (C. gariepinus), including hsp70, cxc, tnfα, il1β, and lysC. More than a 10-fold increase in expression rates was observed for hsp70 in gonads and liver; for cxc in muscles and gonads; for tnfα in brain, gills, and liver; for il1β in the brain, gills, gonads, and liver; and for lysC in gills, gonads, liver, and muscles. This study provides evidence that probiotics exhibiting antioxidant and antimutagenic properties can provide significant benefits in vivo within aquaculture systems. The molecular effects of these probiotics appear to be complex and tissue-specific, with both upregulation and downregulation of immune system genes observed. Nevertheless, at the organismal level, the impact was unequivocally positive in terms of aquaculture objectives, manifested as enhanced body weight gain in the fish. Consequently, these Bacillus strains warrant serious consideration as potential probiotics for this species.

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Supplementation of Bacillus subtilis and Lactobacillus casei to increase growth performance and immune system of catfish (Clarias gariepinus) due to Aeromonas hydrophila infection

Cited by 2 publications

References 65 publications

DIETARY PROBIOTICS AND ITS EFFECT ON GROWTH RATE, SURVIVAL RATE, AND FEED CONVERSION RATIO OF Clarias gariepinus

DIETARY PROBIOTICS AND ITS EFFECT ON GROWTH RATE, SURVIVAL RATE, AND FEED CONVERSION RATIO OF Clarias gariepinus

Potential Probiotic Bacillus Strains with Antioxidant and Antimutagenic Activity Increased Weight Gain and Altered hsp70, cxc, tnfα, il1β, and lysC Gene Expression in Clarias gariepinus

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