Supplementation of Bouea macrophylla Fruit Juice Prevent Oxidative Stress in Rats Fed with High-Fat High-Cholesterol Diet through Attenuation of Lipid Peroxidation

Abstract

Fruits are rich in fiber and antioxidant compounds that can prevent and treat health disorders related to oxidative stress caused by high-fat high-cholesterol diet (HFHCD). Bouea macrophylla is a tropical fruit plant with edible juicy fruits. In this study, we conducted experiments to prove the effectiveness of B. macrophylla fruit juice (BMFJ) as an antioxidant agent in rats fed with HFHCD. Male Sprague Dawley rats were fed with HFHCD for 100 days and simultaneously orally supplemented with BMFJ. Atorvastatin was used as a positive control. At the end of the experiment, the blood and hepar were collected and assayed for malondialdehyde (MDA). The in vitro antioxidant activity of BMFJ was also evaluated using the DPPH method. Total phenols and flavonoids contents were determined using Folin Ciocalteu and AlCl3 methods, respectively. Results of the experiments showed that total phenols and flavonoids in BMFJ were 570 mg GAE/g and 31.89 mg QE/g, respectively, and the IC50 of radical scavenging activity was 564.271 ppm. The in vivo antioxidant evaluation showed that supplementation of BMFJ significantly prevents the increase of MDA levels, both in serum and liver of rats fed with HFHCD. These findings clearly indicate that supplementation of B. macrophylla fruit juice significantly prevents oxidative stress in rats fed with high-fat high-cholesterol diets through attenuation of lipid peroxidation.

Antidiabetic Effect of Chinese yam (Dioscorea opposita Thunb.) in High‐Fat Diet/Streptozotocin‐Induced Diabetic Rats

“…Superoxide dismutase is an important antioxidant enzyme involved in defense against reactive oxygen species. [ 24 ] Malonaldehyde is the by‐product of lipid peroxidation induced by severe oxidative stress [ 25 ] ; its levels directly reflect the number of free radicals in an individual and the degree of lipid peroxidation and indirectly reflect the degree of cell damage. [ 26 ] Nitric oxide can react with proteins, nucleic acids, and other macromolecules to damage organisms and, in severe cases, can directly cause tissue and cell damage.…”

Total Phenolic and Total Flavonoid Content, Antioxidant Activity, and Nutritional Profile of Ziziphus mauritiana Fruit Juice

“…This means that Z. mauritiana fruit juice phenolics content was around 1 to 12 times higher compared to these other fruit juices. Nevertheless, of course, there are also many fruit juices that have a higher content of phenolics, for example, the total phenolic compounds in freeze-dried juice of Bouea macrophylla fruit juice is 57,000 mg GAE/100g (Effendi et al, 2022) and in Ecballium elaterium, freeze-dried fruit juice is 7870 -10,640 mg GAE/100g (Felhi et al, 2016). Phenolic compounds are natural compounds found in plants and are products of plant secondary metabolism.…”

“…The low antioxidant activity of the fruit juices was reported by many researchers, for example, IC50 of freeze-dried juice of apple (Malus domestica) is 805.11 ppm, Canarium sp. is 370,84 ppm(Utami et al, 2019), and Bouea macrophylla is 564.271 ppm(Effendi et al, 2022).…”