Supplementation of complex milk lipid concentrate (CMLc) improved the memory of aged rats

Guan, Jian; MacGibbon, Alastair; Zhang, Rong; Elliffe, Douglas; Moon, Steve; Liu, Dong-Xu

doi:10.1179/1476830513y.0000000096

Cited by 16 publications

(27 citation statements)

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“…Gelatin cubes were used for administration of the dietary supplements, which were formulated using raspberry flavouring and gelatin (10% w/v) containing sucrose (10% w/v) as previously described [32, 33, 36, 44]. Briefly, the MFGM mixtures were dissolved in water using a food processor and were then mixed with 1 L of gelatin/sucrose mixture at 50 °C.…”

Section: Methodsmentioning

confidence: 99%

“…In particular, MFGM composites including PLs [24], sphingolipids [26, 27] and gangliosides [28–30] have purported positive effects on neuroplasticity. For example, dietary supplementation with bovine-derived MFGM mixtures has recently been reported to promote post-natal neurodevelopment [31–35] and to limit age-associated neural decline in elderly animals [36]. …”

Section: Introductionmentioning

confidence: 99%

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Dietary supplementation with bovine-derived milk fat globule membrane lipids promotes neuromuscular development in growing rats

et al. 2017

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BackgroundThe milk fat globule membrane (MFGM) is primarily composed of polar phospho- and sphingolipids, which have established biological effects on neuroplasticity. The present study aimed to investigate the effect of dietary MFGM supplementation on the neuromuscular system during post-natal development.MethodsGrowing rats received dietary supplementation with bovine-derived MFGM mixtures consisting of complex milk lipids (CML), beta serum concentrate (BSC) or a complex milk lipid concentrate (CMLc) (which lacks MFGM proteins) from post-natal day 10 to day 70.ResultsSupplementation with MFGM mixtures enriched in polar lipids (BSC and CMLc, but not CML) increased the plasma phosphatidylcholine (PC) concentration, with no effect on plasma phosphatidylinositol (PI), phosphatidylethanolamine (PE), phosphatidylserine (PS) or sphingomyelin (SM). In contrast, muscle PC was reduced in rats receiving supplementation with both BSC and CMLc, whereas muscle PI, PE, PS and SM remained unchanged. Rats receiving BSC and CMLc (but not CML) displayed a slow-to-fast muscle fibre type profile shift (MyHCI → MyHCIIa) that was associated with elevated expression of genes involved in myogenic differentiation (myogenic regulatory factors) and relatively fast fibre type specialisation (Myh2 and Nfatc4). Expression of neuromuscular development genes, including nerve cell markers, components of the synaptogenic agrin–LRP4 pathway and acetylcholine receptor subunits, was also increased in muscle of rats supplemented with BSC and CMLc (but not CML).ConclusionsThese findings demonstrate that dietary supplementation with bovine-derived MFGM mixtures enriched in polar lipids can promote neuromuscular development during post-natal growth in rats, leading to shifts in adult muscle phenotype.Electronic supplementary materialThe online version of this article (doi:10.1186/s12986-017-0161-y) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dietary supplementation with bovine-derived milk fat globule membrane lipids promotes neuromuscular development in growing rats

et al. 2017

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“…Complex milk lipids (CML), as a source of GA, have potential benefits for brain development. [19][20][21] The rate of GA accretion in the developing brain is highest in utero and in early neonatal life. 22 23 Preclinical research has shown that CML are transferred across the placenta and as such, an increased maternal intake of CML in pregnancy has been associated with increases in fetal GA levels.…”

Section: Introductionmentioning

confidence: 99%

The CLIMB (Complex Lipids In Mothers and Babies) study: protocol for a multicentre, three-group, parallel randomised controlled trial to investigate the effect of supplementation of complex lipids in pregnancy, on maternal ganglioside status and subsequent cognitive outcomes in the offspring

Huang

Norris

et al. 2017

BMJ Open

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IntroductionComplex lipids are important constituents of the central nervous system. Studies have shown that supplementation with complex milk lipids (CML) in pregnancy may increase the level of fetal gangliosides (GA), with the potential to improve cognitive outcomes.Methods and analysisWe aim to recruit approximately 1500 pregnant women in the first trimester (11–14 weeks) and randomise them into one of the three treatment groups: standard maternal milk formulation, CML-enhanced maternal milk formulation or no maternal milk intervention with standard pregnancy advice (ie, the standard care). Maternal lifestyle and demographic data will be collected throughout the pregnancy, as well as biological samples (eg, blood, hair, urine, buccal smear, cord blood, cord and placenta samples). Data from standard obstetric care recorded in hospital maternity notes (eg, ultrasound reports, results of oral glucose tolerance test and pregnancy outcome data) will also be extracted. Postnatal follow-up will be at 6 weeks and 12 months of age, at which point infant cognitive development will be assessed (Bayley Scales of Infant Development I).Ethics and disseminationThis project was approved by the Ethics Committee of Chongqing Medical University. Dissemination of findings will take the form of publications in peer-reviewed journals and presentations at national and international conferences.Trial registration numberChiCTR-IOR-16007700; Pre-results.

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“…The changes in synaptic plasticity in these brain regions, for example, dopamine outputs and glutamate receptors, may be the biological modifications underlying the observed improvements in learning and memory ( 8 , 9 , 11 ). Synaptophysin is a marker for synaptic vesicles and has been commonly used as a marker for synaptic plasticity at pre-synaptic location ( 12 , 13 ). Improved dopamine neurotransmission has been reported to be associated with improved memory in aged rats after long-term supplementation with a concentrate of dairy lipids ( 12 ).…”

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“…Synaptophysin is a marker for synaptic vesicles and has been commonly used as a marker for synaptic plasticity at pre-synaptic location ( 12 , 13 ). Improved dopamine neurotransmission has been reported to be associated with improved memory in aged rats after long-term supplementation with a concentrate of dairy lipids ( 12 ). In addition to a supporting role for astrocytes in neuronal and synaptic plasticity ( 14 ), neuronal–glial–vascular networking plays an essential role in neuronal function ( 15 ) and myelination ( 16 ).…”

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Supplementation with complex milk lipids during brain development promotes neuroplasticity without altering myelination or vascular density

Guillermo¹,

Yang²,

Vickers³

et al. 2015

Food & Nutrition Research

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BackgroundSupplementation with complex milk lipids (CML) during postnatal brain development has been shown to improve spatial reference learning in rats.ObjectiveThe current study examined histo-biological changes in the brain following CML supplementation and their relationship to the observed improvements in memory.DesignThe study used the brain tissues from the rats (male Wistar, 80 days of age) after supplementing with either CML or vehicle during postnatal day 10–80. Immunohistochemical staining of synaptophysin, glutamate receptor-1, myelin basic protein, isolectin B-4, and glial fibrillary acidic protein was performed. The average area and the density of the staining and the numbers of astrocytes and capillaries were assessed and analysed.ResultsCompared with control rats, CML supplementation increased the average area of synaptophysin staining and the number of GFAP astrocytes in the CA3 sub-region of the hippocampus (p<0.01), but not in the CA4 sub-region. The supplementation also led to an increase in dopamine output in the striatum that was related to nigral dopamine expression (p<0.05), but did not alter glutamate receptors, myelination or vascular density.ConclusionCML supplementation may enhance neuroplasticity in the CA3 sub-regions of the hippocampus. The brain regions-specific increase of astrocyte may indicate a supporting role for GFAP in synaptic plasticity. CML supplementation did not associate with postnatal white matter development or vascular remodelling.

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Supplementation of complex milk lipid concentrate (CMLc) improved the memory of aged rats

Abstract: The data suggested that the supplementation of CMLc during the early stage of brain aging may prevent memory decline possibly through improving vascular and neuronal function.

Cited by 16 publications

References 23 publications

Dietary supplementation with bovine-derived milk fat globule membrane lipids promotes neuromuscular development in growing rats

Dietary supplementation with bovine-derived milk fat globule membrane lipids promotes neuromuscular development in growing rats

The CLIMB (Complex Lipids In Mothers and Babies) study: protocol for a multicentre, three-group, parallel randomised controlled trial to investigate the effect of supplementation of complex lipids in pregnancy, on maternal ganglioside status and subsequent cognitive outcomes in the offspring

Supplementation with complex milk lipids during brain development promotes neuroplasticity without altering myelination or vascular density

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