Supplementation of fructose in chemically defined protein-free medium enhances the <i>in vitro</i> development of bovine transgenic cloned embryos

Bhuiyan, Mohammad Musharraf Uddin; Kang, SK; Bc, Lee

doi:10.1017/s0967199407004236

Cited by 8 publications

(7 citation statements)

References 48 publications

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“…Thus, the present hypothesis of the synergistic effect of glucose and fructose in the culture medium might be a logical approach. Most recently, Bhuiyan et al [31] reported that combined glucose and fructose supplementation in the culture medium significantly increased blastocyst formation in bovine transgenic cloned embryos. The expression of Glut5, which has a high affinity for fructose, indicates that the early embryo is capable of transporting this energy substrate [18].…”

Section: Discussionmentioning

confidence: 99%

The beneficial effect of fructose and glucose on in vitro maturation and the fertilization of porcine oocytes

Tsujii

Lee

Hossain

et al. 2008

Reprod Medicine & Biology

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Purpose This study was conducted to investigate the effects of supplementing fructose in the culture medium on in vitro maturation (IVM), in vitro fertilization (IVF), and metabolism of porcine oocytes. Methods Porcine oocytes were matured in vitro in modified North Carolina State University 37 medium (NCSU-37) and then supplemented with either glucose (5.5 mM), fructose (5.5 mM), or glucose (2.75 mM) plus fructose (2.75 mM). The maturation and fertilization of oocytes, and the incorporation and oxidation of 14 C-glucose, 14 Cfructose, and 14 C-methionine in oocytes at different stages of development were examined. Results The supplementation of glucose plus fructose significantly promoted (P \ 0.05) oocytes germinal vesicle break down (GVBD), maturation to metaphase II (MII), penetration by spermatozoa, and male pronuclear formation compared with glucose. The incorporation and oxidation of 14 C-methionine into the oocyte significantly increased (P \ 0.05) with glucose plus fructose supplementation than glucose. A significantly higher (P \ 0.05) rate of incorporation and oxidation was achieved with 14 Cfructose compared to 14 C-glucose. Conclusions Glucose plus fructose supplementation improved maturation, penetration by spermatozoa, male pronuclear formation, and energy metabolism by porcine oocytes.

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Section: Discussionmentioning

confidence: 99%

The beneficial effect of fructose and glucose on in vitro maturation and the fertilization of porcine oocytes

Tsujii

Lee

Hossain

et al. 2008

Reprod Medicine & Biology

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“…In this study, the glycosaminoglycan and type II collagen levels were higher in the serum-free medium than those in the serumsupplemented medium, and the final cell-matrix development was indentified to be suitable (Reza and Nicoll, 2010). Bhuiyan et al (2007) added glucose and fructose to a protein-free serum-free medium for oocytes and determined the ratio of the medium required to optimize the development of bovine transgenic cloned embryos. Studies have also been conducted to reveal that oocyte growth and activation can be induced by the addition of activin or folliclestimulating hormone, even without serum (McLaughlin and Telfer, 2010).…”

Section: Replacing or Removing Fbs In Cell Culture Mediamentioning

confidence: 97%

Review of the Current Research on Fetal Bovine Serum and the Development of Cultured Meat

Lee¹,

Lee²,

Yun³

et al. 2022

Food Sci Anim Resour

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“…A primary activation treatment with electric stimulus, ethanol, ionomycin or calciumionophore induces a rise of the Ca 2+ concentration in oocytes; with strontium, it induces calcium oscillation. Among these primary activation treatments, bovine oocytes are mainly activated with ionomycin after reconstruction [28][29][30]. Ikumi et al [10] used ionomycin as the primary activation treatment for generation of Antarctic minke whale iSCNT embryos using bovine cytoplasts with an adequate proportion (71-86%) of PPN formation.…”

Section: Production Of Sei Whale Cloned Embryosmentioning

confidence: 99%

Production of Sei Whale (Balaenoptera borealis) Cloned Embryos by Inter- and Intra-Species Somatic Cell Nuclear Transfer

Bhuiyan

Suzuki

Watanabe

et al. 2010

Journal of Reproduction and Development

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Abstract. The objectives of this study were to choose an effective embryo reconstruction method and an effective postactivation agent for in vitro production of sei whale (Balaenoptera borealis) interspecies somatic cell nuclear transfer (iSCNT) embryos. Moreover, trichostatin A (TSA) treatment of whale iSCNT embryos was performed to improve the in vitro embryo development. In Experiment 1, the fusion rate was significantly higher (88.1%) in embryos reconstructed using the intracytoplasmic cell injection method (ICI) than that (48.7%) in the subzonal cell insertion (SUZI) counterpart. The rates of pseudopronucleus (PPN) formation (77.4 vs. 77.2%) and cleavage (24.5 vs. 37.0%) did not vary between ICI and SUZI. However, the PPN formation and cleavage rates were significantly (P<0.05) lower in the iSCNT embryos than in the parthenogenetic control (95.7% and 64.4%, respectively). Although 21.5% of the bovine parthenogenetic embryos developed to the blastocyst stage, no iSCNT embryo developed beyond the 6-cell stage. In Experiment 2, the cleavage rate did not vary between the TSA (50 nM)-treated and non-treated whale iSCNT embryos (30.5 vs. 32.3%, respectively). Moreover, it did not vary between the TSA-treated iSCNT and SCNT embryos (30.5 vs. 32.0%, respectively). Only one TSA non-treated iSCNT embryo developed to a compacted morula with 20 nuclei. One TSAtreated whale SCNT embryo developed to the 8-cell stage, and out of five whale iSCNT embryos, a 6-cell stage embryo was positive for whale DNA. In conclusion, bovine oocytes have the ability to support development of sei whale nuclei up to the 6-cell stage. Key words: Interspecies, Somatic cell nuclear transfer, Whale (J. Reprod. Dev. 56: [131][132][133][134][135][136][137][138][139] 2010) esearch on in vitro maturation (IVM), in vitro fertilization (IVF), somatic cell nuclear transfer (SCNT) and in vitro culture (IVC) of whale embryos will greatly contribute to our basic understanding of whale reproductive physiology, resulting, ideally, in the application of various assisted reproductive technologies (ARTs) to increase the population and aid in management of cetaceans. Several studies on ARTs with respect to IVM [1][2][3][4][5][6], cryopreservation of oocytes [2,5,7], IVF [3,8], intracytoplasmic sperm injection (ICSI) [5,6,9] and interspecies SCNT [10] have been conducted in Common minke (Balaenoptera acutorostrata) and Antarctic minke (B. bonaerensis) whales. Recently, we attempted for the first time to produce embryos of sei (B. borealis) whales by IVF of IVM oocytes in a research based ship [11]. However, there is no information available concerning the production of SCNT embryos in sei whales. The authors had the opportunity to board a vessel in the North Pacific Ocean in 2007 to collect a sei whale fetus and to establish a fetal fibroblast cell line for an attempt to produce whale cloned embryos by SCNT. It has been demonstrated elsewhere that bovine, porcine and rabbit oocytes can support remodeling and reprogramming of somatic cells from different sp...

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Supplementation of fructose in chemically defined protein-free medium enhances the in vitro development of bovine transgenic cloned embryos

Cited by 8 publications

References 48 publications

The beneficial effect of fructose and glucose on in vitro maturation and the fertilization of porcine oocytes

The beneficial effect of fructose and glucose on in vitro maturation and the fertilization of porcine oocytes

Review of the Current Research on Fetal Bovine Serum and the Development of Cultured Meat

Production of Sei Whale (Balaenoptera borealis) Cloned Embryos by Inter- and Intra-Species Somatic Cell Nuclear Transfer

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