Supplementation of Aspergillus glaucus with gfdB gene encoding a glycerol 3‐phosphate dehydrogenase in Aspergillus nidulans

Király, Anita; Szabó, Ivett G.; Emri, Tamás; Leiter, Éva; Pócsi, István

doi:10.1002/jobm.202000067

Cited by 4 publications

(15 citation statements)

References 28 publications

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“…The copy number of the gfdB gene incorporated in the A. wentii genome was quantified as previously described {(Király et al 2020a , b ; Szabó et al 2020a , b ); using the equation of (Herrera et al 2009 )}. …”

Section: Methodsmentioning

confidence: 99%

“…The following stress-eliciting agents were employed at the concentrations indicated in parentheses: cell wall integrity stress: Congo Red (54, 81, and 108 μM); oxidative stress: tert -butyl hydroperoxide ( t BOOH; 0.4, 1.6, and 2.4 mM), hydrogen peroxide (9 and 18 mM), menadione sodium bisulphite (MSB; 0.096, 0.19, 0.38, 0.62 mM), diamide (1.5 mM); heavy metal stress: CdCl 2 (0.1, 0.15, 0.2, and 0.5 mM); hyperosmotic stress (when this tearm is applicable)): sorbitol (2 M), NaCl (0.5, 1, and 1.5 mM). Following stress treatments, the stress sensitivity of the strains was characterized by the diameters of the colonies (Balázs et al 2010 ; de Vries et al 2017 ; Orosz et al 2018 ; Király et al 2020a , b ).…”

Section: Methodsmentioning

confidence: 99%

“…To perform cluter analysis on the general stress sensitivity observed in the genus of the aspergilli, growths of the A. wentii (CBS141173), A. wentii (CBS141173) 'c gfdB1 , A. glaucus (CBS516.65), A. glaucus (CBS516.65) 'c gfdB1 , A. nidulans THS30.3, and A. nidulans ΔgfdB strains recorded in current and pervious studies (Király et al 2020a , b ) were compared to growth data gained with other Aspergillus spp. and deposited in the Fungal Stress Database (FSD; https://www.fung-stress.org/ ; de Vries et al 2017 ; Orosz et al 2018 ; Emri et al 2018 ).…”

Section: Methodsmentioning

confidence: 99%

“…In A. glaucus , the implementation and expression of A. nidulans gfdB with its own promoter increased considerably the oxidative, cell wall integrity, and heavy metal (Cd 2+ ) stress tolerance of the fungus cultured on 2 M sorbitol without affecting its osmophily (Király et al 2020b ). Because the adequate stress tolerance of industrial yeasts and filamentous fungi is of paramount importance in fermentation processes (Bai et al 2003 ; Li et al 2011 ; Teixeira et al 2011 ; Hagiwara et al 2016 ; Deparis et al 2017 ; Steensels et al 2019 ; Brandt et al 2021 ; Yaakoub et al 2022 ), we wanted to test if the genetic transfer of A. nidulans gfdB to A. wentii with its own promoter would also enhace the general stress tolerance of this fungus.…”

Section: Introductionmentioning

confidence: 99%

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Species-specific effects of the introduction of Aspergillus nidulans gfdB in osmophilic aspergilli

Bodnár

Király

Orosz

et al. 2023

Appl Microbiol Biotechnol

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Industrial fungi need a strong environmental stress tolerance to ensure acceptable efficiency and yields. Previous studies shed light on the important role that Aspergillus nidulans gfdB, putatively encoding a NAD+-dependent glycerol-3-phosphate dehydrogenase, plays in the oxidative and cell wall integrity stress tolerance of this filamentous fungus model organism. The insertion of A. nidulans gfdB into the genome of Aspergillus glaucus strengthened the environmental stress tolerance of this xerophilic/osmophilic fungus, which may facilitate the involvement of this fungus in various industrial and environmental biotechnological processes. On the other hand, the transfer of A. nidulans gfdB to Aspergillus wentii, another promising industrial xerophilic/osmophilic fungus, resulted only in minor and sporadic improvement in environmental stress tolerance and meanwhile partially reversed osmophily. Because A. glaucus and A. wentii are phylogenetically closely related species and both fungi lack a gfdB ortholog, these results warn us that any disturbance of the stress response system of the aspergilli may elicit rather complex and even unforeseeable, species-specific physiological changes. This should be taken into consideration in any future targeted industrial strain development projects aiming at the fortification of the general stress tolerance of these fungi. Key points • A. wentii c’ gfdB strains showed minor and sporadic stress tolerance phenotypes. • The osmophily of A. wentii significantly decreased in the c’ gfdB strains. • Insertion of gfdB caused species-specific phenotypes in A. wentii and A. glaucus.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Species-specific effects of the introduction of Aspergillus nidulans gfdB in osmophilic aspergilli

Bodnár

Király

Orosz

et al. 2023

Appl Microbiol Biotechnol

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“…The amplicons were digested with restriction enzymes as indicated in Supplementary Table S2, and ligated between the niiA promoter and the trpC terminator in pHS11 (Leiter et al, 2016). Overexpression of the strains was confirmed by rRT-PCR method (Supplementary Figure S1; Király et al, 2020b).…”

Section: Construction Of Gene Deletion and Overexpression Strainsmentioning

confidence: 99%

Functional analysis of the bZIP-type transcription factors AtfA and AtfB in Aspergillus nidulans

Kocsis

Lee

et al. 2022

Front. Microbiol.

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Transcription factors (TFs) with the basic leucin zipper domain are key elements of the stress response pathways in filamentous fungi. In this study, we functionally characterized the two bZIP type TFs AtfA and AtfB by deletion (Δ) and overexpression (OE) of their encoding genes in all combination: ΔatfA, ΔatfB, ΔatfAΔatfB, ΔatfAatfBOE, ΔatfBatfAOE, atfAOE, atfBOE and atfAOEatfBOE in Aspergillus nidulans. Based on our previous studies, ΔatfA increased the sensitivity of the fungus to oxidative stress mediated by menadione sodium bisulfite (MSB) and tert-butylhydroperoxide (tBOOH), while ΔatfB was not sensitive to any oxidative stress generating agents, namely MSB, tBOOH and diamide at all. Contrarily, the ΔatfB mutant was sensitive to NaCl, but tolerant to sorbitol. Overexpression of atfB was able to compensate the MSB sensitivity of the ΔatfA mutant. Heavy metal stress elicited by CdCl2 reduced diameter of the atfBOE and atfAOEatfBOE mutant colonies to about 50% of control colony, while the cell wall stress generating agent CongoRed increased the tolerance of the ΔatfA mutant. When we tested the heat stress sensitivity of the asexual spores (conidiospores) of the mutants, we found that conidiospores of ΔatfAatfBOE and ΔatfBatfAOE showed nearly 100% tolerance to heat stress. Asexual development was negatively affected by ΔatfA, while atfAOE and atfAOE coupled with ΔatfB increased the number of conidiospores of the fungus approximately 150% compared to the control. Overexpression of atfB led to a 25% reduction in the number of conidiospores, but increased levels of abaA mRNA and size of conidiospores. Sexual fruiting body (cleistothecium) formation was diminished in the ΔatfA and the ΔatfAΔatfB mutants, while relatively elevated in the ΔatfB and the ΔatfBatfAOE mutants. Production of the mycotoxin sterigmatocystin (ST) was decreased to undetectable levels in the ΔatfA mutant, yet ST production was restored in the ΔatfAΔatfB mutant, suggesting that ΔatfB can suppress ST production defect caused by ΔatfA. Levels of ST were also significantly decreased in the ΔatfAatfBOE, ΔatfBatfAOE and atfAOEatfBOE mutants.

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Biotechnological potential of salt tolerant and xerophilic species of Aspergillus

Pócsi,

Dijksterhuis,

Houbraken

et al. 2024

Appl Microbiol Biotechnol

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Xerophilic fungi occupy versatile environments owing to their rich arsenal helping them successfully adapt to water constraints as a result of low relative humidity, high-osmolarity, and high-salinity conditions. The general term xerophilic fungi relates to organisms that tolerate and/or require reduced water activity, while halophilic and osmophilic are applied to specialized groups that require high salt concentrations or increased osmotic pressure, respectively. Species belonging to the family Aspergillaceae, and especially those classified in Aspergillus subgenus Aspergillus (sections Restricti and Aspergillus) and Polypaecilum, are particularly enriched in the group of osmophilic and salt-tolerant filamentous fungi. They produce an unprecedently wide spectrum of salt tolerant enzymes including proteases, peptidases, glutaminases, γ-glutamyl transpeptidases, various glycosidases such as cellulose-decomposing and starch-degrading hydrolases, lipases, tannases, and oxidareductases. These extremophilic fungi also represent a huge untapped treasure chest of yet-to-be-discovered, highly valuable, biologically active secondary metabolites. Furthermore, these organisms are indispensable agents in decolorizing textile dyes, degrading xenobiotics and removing excess ions in high-salt environments. They could also play a role in fermentation processes at low water activity leading to the preparation of daqu, meju, and tea. Considering current and future agricultural applications, salt-tolerant and osmophilic Aspergilli may contribute to the biosolubilization of phosphate in soil and the amelioration salt stress in crops. Transgenes from halophile Aspergilli may find promising applications in the engineering of salt stress and drought-tolerant agricultural crops. Aspergilli may also spoil feed and food and raise mycotoxin concentrations above the permissible doses and, therefore, the development of novel feed and food preservation technologies against these Aspergillus spp. is also urgently needed. On the other hand, some xerophilic Aspergilli have been shown to be promising biological control agents against mites. Key points • Salt tolerant and osmophilic Aspergilli can be found in versatile environments • These fungi are rich resources of valuable enzymes and secondary metabolites • Biotechnological and agricultural applications of these fungi are expanding

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Supplementation of Aspergillus glaucus with gfdB gene encoding a glycerol 3‐phosphate dehydrogenase in Aspergillus nidulans

Cited by 4 publications

References 28 publications

Species-specific effects of the introduction of Aspergillus nidulans gfdB in osmophilic aspergilli

Species-specific effects of the introduction of Aspergillus nidulans gfdB in osmophilic aspergilli

Functional analysis of the bZIP-type transcription factors AtfA and AtfB in Aspergillus nidulans

Biotechnological potential of salt tolerant and xerophilic species of Aspergillus

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