Supplementation of ram's semen extender with Mito-TEMPO I: Improvement in quality parameters and reproductive performance of cooled-stored semen

Zarei, F.; Kia, Hossein Daghigh; Masoudi, Reza; Moghaddam, Gholamali; Ebrahimi, Mohammad

doi:10.1016/j.cryobiol.2020.10.018

Cited by 33 publications

(32 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regarding the special anatomy of cervix in sheep (Masoudi et al, 2012), the sperm quality is important to improve fertility rate after artificial insemination. Moreover, in our previous study in preservation of ram semen during cooling storage (Zarei et al, 2021), Mito-TEMPO preserved cooled spermatozoon and improved semen quality and fertility potential, so the current study was conducted to evaluate Mito-TEMPO helpful effects during ram semen cryopreservation. Therefore, sperm motility, malondialdehyde (MDA) content, membrane functionality, morphology, mitochondria activity, DNA fragmentation, ROS concentration, acrosome integrity, viability and apoptotic-like changes were assessed to find and report the positive impacts of Mito-TEMPO in ram semen cryopreservation.…”

Section: The Novel Cell Permeable Targeted Antioxidant In Mitochondriamentioning

confidence: 99%

Supplementation of ram’s semen extender with Mito‐TEMPO II: Quality evaluation and flow cytometry study of post‐thawed spermatozoa

2021

View full text Add to dashboard Cite

Cryopreservation is an effective method to spread qualified ram spermatozoa for reproductive goals in different farms, but cryopreservation's shocks reduce sperm quality. This study investigated the efficacy of the new mitochondria‐targeted antioxidant Mito‐TEMPO on post‐thawed quality of spermatozoa in sheep. Collected samples were divided into five groups and after dilution, received different doses of Mito‐TEMPO (0, 0.5, 5, 50 and 500 µM), and frozen. Thawed sperm motility parameters, malondialdehyde content, membrane functionality, abnormal morphology, mitochondria activity, acrosome integrity, DNA fragmentation, ROS concentration, viability and apoptotic‐like changes, were evaluated. According to the results, Mito‐TEMPO (5 and 50 μM) improved (p ≤ 0.05) motility parameters, average path velocity, membrane functionality, mitochondria activity and viability compared with the other groups. Moreover, apoptotic‐like changes, lipid peroxidation and ROS concentration were lower (p ≤ 0.05) in groups received 5 and 50 μM Mito‐TEMPO. Mito‐TEMPO showed no effect (p > 0.05) on sperm acrosome integrity, morphology and DNA fragmentation. In conclusion, Mito‐TEMPO as a targeted antioxidant could be an efficient cryo‐additive to enhance quality parameters of post‐thawed ram semen.

show abstract

Section: The Novel Cell Permeable Targeted Antioxidant In Mitochondriamentioning

confidence: 99%

Supplementation of ram’s semen extender with Mito‐TEMPO II: Quality evaluation and flow cytometry study of post‐thawed spermatozoa

2021

View full text Add to dashboard Cite

show abstract

“…It could be because of the anti‐apoptotic and antioxidant role of acetovanillone as it strengthens the glutathione peroxidase‐glutathione reductase system, which was reported to enhance the progressive motility of bull spermatozoa 61 . Non‐significant difference observed in 50 μM Mito TEMPO–supplemented groups might be because of enhanced antioxidant potential of sperm mitochondria, which has an important role in sperm motility as reported in human, 14,17 boar, 16 rooster, 15 and ram 18 …”

Section: Discussionmentioning

confidence: 86%

Supplementation of Mito TEMPO and acetovanillone in semen extender improves freezability of buffalo spermatozoa

et al. 2022

View full text Add to dashboard Cite

Background: Oxidative stress is one of the leading factors responsible for poor postthaw semen quality because of overproduction of reactive oxygen species (ROS) over neutralizing antioxidants present in semen. Mainly two ROS generation sites are present in spermatozoa, that is, mitochondria and plasma membrane. Therefore, the idea of targeting these specific sites for minimization of ROS production with the compounds having known mechanism of actions was built up as a core for this research.Objective: Present study was done to investigate the effects of Mito TEMPO and acetovanillone individually and in combination on freezability of buffalo spermatozoa. Materials and Methods:For the experiment, semen extender was supplemented with Mito TEMPO (50 μM), acetovanillone (50 μM), and a combination of Mito TEMPO + acetovanillone (50 μM+ 50 μM), designated as Group II, Group III, and Group IV, respectively. Control group without any supplementation was designated as Group I. A total of 24 ejaculates with individual progressive motility (IPM) of ≥70% were selected for the study. After final dilution, filling-sealing of straws, equilibration, and freezing were done as per the standard procedure. Semen samples were evaluated for IPM, plasma membrane integrity, lipid peroxidation, total antioxidant capacity (TAC), and cholesterol to phospholipids (C/P) ratio at both fresh and post-thaw stages. Evaluation of ROS, mitochondrial membrane potential (MMP), capacitation status (CTC assay), and in vitro fertility potential were conducted only on frozen-thawed samples.Results: The addition of Mito TEMPO (50 μM) and acetovanillone (50 μM) individually and in combination significantly (p < 0.05) improved post-thaw semen quality in terms of IPM, plasma membrane integrity, TAC, cholesterol content, C/P ratio, MMP, Chlortetracycline (CTC)-Full (F) pattern, and zona binding ability of buffalo spermatozoa, while significantly (p < 0.05) reduced ROS production, lipid peroxidation, and capacitation like changes as compared to the control group.Discussion: As Mito TEMPO acts as an SOD mimetic and also detoxifies ferrous iron at the mitochondria level, it aids in neutralization of excessive ROS production and minimizes oxidative stress-related damages that enhances the antioxidant potential of

show abstract

“…These positive results were ascribed to the capacity of Omega 3 fatty acids to reduce the deleterious effects of oxidative stress and maintain the physical and kinematic properties of sperm cells. In the same vein, it has been reported that enrichment of egg yolk with PUFAs, particularly flaxseed oil or fish oil can preserve the quality of ram semen during liquid storage to a level whereby it can still be used with success in cervical transfers [35].…”

Section: Supplementation Of Extenders With Fatty Acidsmentioning

confidence: 96%

“…More recently, using 5 and 50 µM of Mito-Tempo as a supplement to cooling medium has shown to improve sperm total motility, progressive motility, membrane functionality, viability and lower MDA concentration during 24 and 48 h storage. Also, pregnancy, parturition and lambing rates have shown to be higher when ewes were inseminated with 24 h-chilled semen samples treated with Mito-TEMPO (5 and 50 µM) compared to the control group[35].…”

mentioning

confidence: 99%

Liquid Storage of Ram Semen: Associated Damages and Improvement

Kameni¹,

Meutchieye²,

Ferdinand³

2021

OJAS

View full text Add to dashboard Cite

show abstract

Supplementation of ram's semen extender with Mito-TEMPO I: Improvement in quality parameters and reproductive performance of cooled-stored semen

Cited by 33 publications

References 27 publications

Supplementation of ram’s semen extender with Mito‐TEMPO II: Quality evaluation and flow cytometry study of post‐thawed spermatozoa

Supplementation of ram’s semen extender with Mito‐TEMPO II: Quality evaluation and flow cytometry study of post‐thawed spermatozoa

Supplementation of Mito TEMPO and acetovanillone in semen extender improves freezability of buffalo spermatozoa

Liquid Storage of Ram Semen: Associated Damages and Improvement

Contact Info

Product

Resources

About