Supplementation of zinc on oocyte in vitro maturation improves preimplatation embryonic development in pigs

Jeon, Yubyeol; Yoon, Jong Hwan; Cai, Lei; Hwang, Seon‐Ung; Kim, Eunhye; Zheng, Zhong; Lee, Eunsong; Kim, Dae Young; Hyun, Sang‐Hwan

doi:10.1016/j.theriogenology.2014.06.021

Cited by 44 publications

(31 citation statements)

References 46 publications

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“…However, the production efficiency of cloned pigs increased in the group that received zinc supplementation during IVM. The beneficial effects of zinc supplementation during IVM on oocyte maturation and embryonic developmental competence were demonstrated in previous report [12]. Specifically, zinc supplementation during IVM increased intracellular GSH synthesis, reduced ROS levels, and improved transcription factor expression.…”

supporting

confidence: 55%

“…The selected COCs were washed three times in HEPES-buffered Tyrode’s medium containing 0.05% (w/v) polyvinyl alcohol (TLH-PVA), and transferred to 500 μl of tissue culture medium 199 (Invitrogen, Carlsbad, CA, USA) supplemented with 0.8 µg/ml zinc (zinc supplemented group only), 0.6 mM cysteine, 0.91 mM sodium pyruvate, 10 ng/ml epidermal growth factor, 75 μg/ml kanamycin, 1 μg/ml insulin, and 10% (v/v) pig follicular fluid. The concentration of zinc was set at 0.8 µg/ml in accordance with previous report [12]. For maturation, the selected COCs were washed three times in the above described tissue culture medium 199 (IVM medium) containing hormone supplements (10 IU/ml equine chorionic gonadotropin and 10 IU/ml human chorionic gonadotropin [Intervet, Boxmeer, the Netherlands]), and approximately 50–60 oocytes were transferred to each well of a 4-well Nunc dish (Roskilde, Denmark) containing 500 μl of culture medium and equilibrated for at least 2 h with 5% CO 2 at 39°C in a humidified atmosphere.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, modified in vitro production systems that more closely mimic the in vivo microenvironment are required to improve the development of porcine embryos. In a previous report, zinc supplementation at a normal body fluid concentration (0.8 µg/ml) during in vitro maturation (IVM) was shown to have beneficial effects on the embryonic development of parthenogenetic and in vitro -fertilized (IVF) embryos [12]. Zinc supplementation during IVM may also be beneficial for the embryonic development of embryos produced by somatic cell nuclear transfer (SCNT) and the production of cloned pigs.…”

mentioning

confidence: 99%

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Zinc supplementation during <i>in vitro</i> maturation increases the production efficiency of cloned pigs

Jeon

Yoon

Cai

et al. 2016

Journal of Reproduction and Development

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Zinc supplementation (0.8 µg/ml) in in vitro maturation (IVM) medium significantly enhances oocyte quality. In this study, we compared the development of somatic cell nuclear transfer (SCNT) embryos produced from conventional IVM (control) and zinc-supplemented IVM oocytes. A total of 1206 and 890 SCNT embryos were produced using control and zinc-supplemented oocytes, respectively, and then were transferred to 11 and 8 recipients, respectively. Five control recipients and three zinc-supplemented recipients became pregnant. Two live piglets and eight mummies were born from two control recipients, and ten live piglets and six stillborn piglets were born from three zinc-supplemented recipients. The production efficiency significantly increased in the zinc-supplemented group (0.33% vs. 3.02%). This report suggests that zinc supplementation in IVM medium improved the production efficiency of cloned pigs.

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supporting

confidence: 55%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Zinc supplementation during <i>in vitro</i> maturation increases the production efficiency of cloned pigs

Jeon

Yoon

Cai

et al. 2016

Journal of Reproduction and Development

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“…The importance of Zn in FF may also derive from its biological role in moderating oxidative stress via mediation of reactive oxygen species (ROS). Zn-supplemented media during in vitro maturation (IVM) of porcine blastocysts significantly decreased ROS levels in mature oocytes, likely in association with increased superoxide dismutase (SOD) activities and higher intracellular glutathione levels [37]. Similarly, Zn media supplementation (3.6-19 μmol) was associated with better quality mouse embryos [38,39], yet higher concentrations (25 μmol) were embryotoxic [39].…”

Section: Discussionmentioning

confidence: 99%

Associations between IVF outcomes and essential trace elements measured in follicular fluid and urine: a pilot study

Ingle

Bloom

Parsons

et al. 2016

J Assist Reprod Genet

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Purpose A hypothesis-generating pilot study exploring associations between essential trace elements measured in follicular fluid (FF) and urine and in vitro fertilization (IVF) endpoints. Methods We recruited 58 women undergoing IVF between 2007 and 2008, and measured cobalt, chromium, copper, manganese, molybdenum, and zinc in FF (n = 46) and urine (n = 45) by inductively coupled plasma mass spectrometry (ICP-MS). We used multivariable regression models to assess the impact of FF and urine trace elements on IVF outcomes, adjusted for age, body mass index, race, and cigarette smoking.Results Trace elements were mostly present at lower concentrations in FF than in urine. The average number of oocytes retrieved was positively associated with higher urine cobalt, chromium, copper, and molybdenum concentrations. FF chromium and manganese were negatively associated with the proportion of mature oocytes, yet urine manganese had a positive association. FF zinc was inversely associated with average oocyte fertilization. Urine trace elements were significant positive predictors for the total number of embryos generated. FF copper predicted lower embryo fragmentation while urine copper was associated with higher embryo cell number and urine manganese with higher embryo fragmentation. No associations were detected for implantation, pregnancy, or live birth. Conclusions Our results suggest the importance of trace elements in both FF and urine for intermediate, although not necessarily clinical, IVF endpoints. The results differed using FF or urine biomarkers of exposure, which may have implications for the design of clinical and epidemiologic investigations. These initial findings will form the basis of a more definitive future study.

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“…A possible reason for this difficulty is an insufficient in vitro maturation (IVM) system. Several researchers have attempted to improve IVM conditions by the addition of microelements [ 3 4 ] . Microelements possibly play important roles in IVM systems [ 5 ] and support female reproduction in many species [ 6 8 ] .…”

Section: Introductionmentioning

confidence: 99%

Effect of zeaxanthin on porcine embryonic development during in vitro maturation

Park

Hyun

2017

J Biomed Res

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Zeaxanthin is a common carotenoid, which is a powerful antioxidant that protects against damage caused by reactive oxygen species. The aim of the present study was to investigate the effects of zeaxanthin supplementation on in vitro maturation of porcine embryo development. We investigated nuclear maturation, intracellular glutathione (GSH), and reactive oxygen species (ROS) levels during in vitro maturation, and subsequent embryonic development following parthenogenetic activation and in vitro fertilization (IVF). The oocytes were maturated and used at the metaphase II stage. After 42 hours of in vitro maturation, the zeaxanthin-treated group (0.5 mmol/L) showed significant increases in nuclear maturation (89.6%) than the control group (83.4%) (P<0.05). The intracellular GSH levels increased significantly (P<0.05) as zeaxanthin concentrations increased; ROS generation levels decreased with increased zeaxanthin concentrations, but there were no significant differences. There were no significant differences in subsequent embryonic development, cleavage rate, blastocyst stage rate, and total blastocyst cell numbers following parthenogenetic activation and IVF when in vitro maturation media was supplemented with zeaxanthin. These results suggest that treatment with zeaxanthin during in vitro maturation improved the nuclear maturation of porcine oocytes by increasing the intracellular GSH level, thereby slightly decreasing the intracellular ROS level.

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Supplementation of zinc on oocyte in vitro maturation improves preimplatation embryonic development in pigs

Cited by 44 publications

References 46 publications

Zinc supplementation during <i>in vitro</i> maturation increases the production efficiency of cloned pigs

Zinc supplementation during <i>in vitro</i> maturation increases the production efficiency of cloned pigs

Associations between IVF outcomes and essential trace elements measured in follicular fluid and urine: a pilot study

Effect of zeaxanthin on porcine embryonic development during in vitro maturation

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