Supplementation with long-acting progesterone in early diestrus in beef cattle: I. effect of artificial insemination on onset of luteolysis

Martins, Thiago; Pugliesi, Guilherme; Sponchiado, Mariana; Cardoso, Beatriz de Oliveira; Gomes, N. S.; Mello, Bárbara Piffero; Celeghini, Eneiva Carla Carvalho; Binelli, Mário

doi:10.1016/j.domaniend.2018.12.002

Cited by 9 publications

(8 citation statements)

References 31 publications

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“…The total number of follicles per pair of ovaries was recorded in three categories: small (<5 mm in diameter), medium (between 5 and 10 mm in diameter), and large follicles (>10 mm in diameter) [25]. The diameters of the dominant follicles, corpus luteum, and the ovaries were assessed by considering the average between measurements of their two perpendicular axes [26]. Pregnancy diagnosis was performed by ultrasonography on day 37 post-AI to assess the pregnancy rate to single AI.…”

Section: Heifer Reproductive Performance During Rearingmentioning

confidence: 99%

Effects of Developmental Programming Caused by Maternal Nutrient Intake on Postnatal Performance of Beef Heifers and Their Calves

Noya

Casasús

Ferrer

et al. 2019

Animals

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In this study, we evaluated the effects of maternal subnutrition in early pregnancy on the growth and reproductive performance of female offspring during their rearing, first gestation, and lactation. We inseminated 21 Parda and 15 Pirenaica multiparous cows and assigned them to a CONTROL (100% of nutrition requirements) or SUBNUT (65%) diet until day 82 of gestation. Cows were fed 100% requirements afterward. During the rearing of female offspring, growth, physiological profiles and ovarian follicular dynamic were studied. At 16 months old, heifers were inseminated. After first calving, dam–calf weights were recorded during lactation. Heifers born from CONTROL cows were heavier at weaning (four months old) than heifers born from SUBNUT cows, but this difference disappeared at the end of rearing and during the first gestation and lactation periods. All heifers reached puberty at a similar age and live weight. During rearing, SUBNUT heifers had higher concentrations of non-esterified fatty acids, urea, and cholesterol and a lower antral follicle count than CONTROL, but no difference was found in their fertility rate. After heifer first calving, dam–calf weights were similar among groups. In conclusion, maternal undernutrition reduced offspring postnatal gains at weaning, compromising metabolic status and follicle population during rearing but did not impair performance in the first gestation and lactation periods of beef heifers.

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Section: Heifer Reproductive Performance During Rearingmentioning

confidence: 99%

Effects of Developmental Programming Caused by Maternal Nutrient Intake on Postnatal Performance of Beef Heifers and Their Calves

Noya

Casasús

Ferrer

et al. 2019

Animals

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“…Apesar da utilização de progesterona injetável (iP4) em protocolos de IATF com fins de otimizar a eficiência reprodutiva em um rebanho demonstrar resultados distintos [10,14,18,24,26], na presente pesquisa, embora a taxa de gestação encontrada seja considerada de baixa fertilidade, a utilização de iP4 aumentou a taxa de gestação, independente da categoria estudada. Esse achado pode ser atribuído à expressão de genes que favorecem o desenvolvimento embrionário e a produção de INFT quando níveis de P4 se elevam no momento propício para o estabelecimento da prenhez [34].…”

Section: Discussionunclassified

“…Todavia, o uso de suplementação de progesterona injetável (iP4) de ação prolongada após a ovulação e fertilização é controverso, uma vez que incrementos nas taxas gestacionais atribuídas ao seu uso dependerá do momento da aplicação da progesterona após a ovulação [36]. Seu uso precoce (D3 após a ovulação), antes da formação completa do corpo lúteo (CL) pode ser prejudicial para os resultados da gestação, causando um CL menor e perda embrionária [8,18,19,35], se utilizado entre os D3 a D7 pós período de inseminação acarreta aumentos na taxa de gestação; por outro lado, se a suplementação ocorrer após o D7 não terá efeito [36].…”

Section: Introductionunclassified

Influence of Injectable Progesterone on the Pregnancy Rate and Gestational Loss of Nellore Females of Different Reproductive Categories Undergoing Fixed-Time Artificial Insemination

Diniz

Dell’Aqua

Oba

et al. 2021

Acta Scientiae. Vet.

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Background:Progesterone (P4) is essential for embryonic development and maintenance of pregnancy when deficiency causes early embryonic loss. In this study, we investigated the ability of hormonal supplementation to improve the fertility of cows subjected to fixed-time artificial insemination (IATF) protocols. Here, we evaluated the effect of long-acting injectable progesterone (iP4) supplementation in the D4 after IATF on pregnancy rate and pregnancy loss in Nellore cows (Bos taurus indicus) from different reproductive categories in Western Amazonia.Materials, Methods & Results:Eight hundred thirteen animals from 5 farms were selected and distributed into 2 groups: control [GC; administration of 0.5 mL of 0.9% saline solution, intramuscularly – IM] (n = 407) and a group that received injectable progesterone (iP4) that was long-acting [GiP4; administration of 0.5 mL of iP4, 300 mg, via IM four days after IATF] (n = 406). Each group contained 3 subgroups: heifers, primiparous cows, and multiparous cows. Of the 407 animals in the CG, 103 were heifers, 107 primiparous, and 197 multiparous. Of the 406 animals in the GiP4 group, there were 101 heifers, 107 primiparous, and 198 multiparous. On a random day of the estrous cycle (D0), an intravaginal device containing 1 g of P4 and 2 mg of estradiol benzoate (BE) was inserted by intramuscular injection. On D8, the P4 device was removed and 150 μg of D-cloprostenol (PGF2α), 300 IU eCG, and 1 mg BE were administered IM. Cows were inseminated at D10, 48-52 h after procedure on D8. Pregnancy diagnosis was made between 35 and 40 days after insemination through ultrasound examination. Between 80 and 90 days after insemination, a new ultrasound examination was performed to assess early pregnancy loss. The data were processed using the SAS 9.2. The conception rate, pregnancy loss, and final conception rate were analyzed using PROC GLIMMIX according to groups (CG and GiP4), categories (heifers, primiparous and multiparous), and their interactions as variables. The differences in the means of least squares were adjusted using the Tukey–Kramer method. Statistical significance was defined as P < 0.05. The general conception rate was 46% (375/816). Regardless of the animal class, GiP4 animals (51.97%) had higher conception rates (P < 0.05) than CG (40.29%). In the subgroups (heifers, primiparous and multiparous cows), there was a difference (P < 0.05) between animals treated with iP4 (52.48%, 57.94%, and 48.48%, respectively) and those who were not (39.81%, 41.12%, and 40.10%, respectively). Gestational losses, regardless of the animal class, were higher in females in the CG (7.93%) [P < 0.05] compared to those in the GiP4 group (2.84%). Regardless of treatment with iP4, the percentage of gestational loss in heifers was significantly higher (10.64%) than that in primiparous and multiparous cows (3.77% and 2.86%, respectively). The final conception rates were higher in animals that received long-acting iP4, which increased the final pregnancy in all parity categories. Discussion: In the present study, the use of iP4 increased the pregnancy rate in Nellore females, regardless of the category. Although there has been no consensus on the use of iP4, there is an agreement that increases in the pregnancy rate are related to the moment of exogenous P4 application. In addition to influencing the pregnancy rate, reduction in pregnancy losses is also attributed to iP4 treatment, a fact demonstrated in the present study, where animals treated with iP4 had a lower pregnancy loss rate than normally occurs in beef cattle. Supplementation with long-acting iP4 increased the pregnancy rate at D35-40, reduced pregnancy losses, and increased the conception rate on D80-90 days in Nellore females reared in the Western Amazon, regardless of the animal category.Keywords: abortion, reproductive biotechnologies, Bos taurus indicus, pregnancy, progesterone supplementation.Descritores: aborto,biotecnologias reprodutivas, Bos taurus indicus, gestação, suplementação de progesterona.Título: Influência da progesterona injetável na taxa de prenhez e perda gestacional de fêmeas nelore de distintas categorias reprodutivas submetidas a inseminação artificial em tempo fixo.

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“…Similarly, in dairy cows (Monteiro Jr et al, 2015), the pregnancy rate did not differ (p > .05) between cows receiving a 1.9 g P4 device from 3 to 17 days after FTAI (32%) and untreated cows (31.8%). In contrast, dairy cows submitted to FTET programs had a lesser pregnancy rate when they received a P4 intravaginal device (1.9 g) from (Batista et al, 2019;Martins et al, 2019) in beef cows supplemented with 150-300 mg iP4 on the third day post-ovulation. Furthermore, the occurrence of early luteolysis was associated with an increased plasma P4 profile in the first 3 days post-iP4 treatment (Batista et al, 2019).…”

Section: F I G U R Ementioning

confidence: 92%

“…Regarding the effects on pregnancy rates, the majority of studies evaluated iP4 supplementation only in dairy (Kumar et al, 2012;Souza, 2015;Stronge et al, 2005) and beef (Martins et al, 2019;Pugliesi et al, 2016) females submitted to fixed-time AI (FTAI) programs. Yan et al (2016) in a meta-analysis study concluded that supplementation with P4 improves conception rates in beef or dairy females submitted to FTAI, but only when performed at early dioestrus (days 3-7 post-oestrus).…”

Section: Introductionmentioning

confidence: 99%

Effects of long‐acting injectable progesterone supplementation at early dioestrus on pregnancy maintenance in beef and dairy recipient cattle

Neto,

Ruas,

Santo

et al. 2023

Reprod Domestic Animals

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We tested in the present study the hypothesis that supplementation with long‐acting P4 (iP4) at different times of the initial dioestrus improves pregnancy rates in dairy and beef recipients submitted to fixed‐time embryo transfer (FTET). Recipients from commercial farms had their oestrous cycle synchronized with an E2/P4‐based protocol in three experiments (Exp. 1 to 3). In Exp. 1, dairy heifers (n = 76) and cows (n = 104) were randomly assigned to two experimental groups: the control group (n = 89) and the iP4D4 group (n = 91). For Exps. 2 and 3, suckled beef recipients were used. In Exp. 2, recipients were assigned to two experimental groups: Control group (n = 147) and iP4D7 group (n = 144); whereas in Exp. 3, recipients were randomly assigned to three experimental groups: Control group (n = 85), iP4‐D4 group (n = 86) and iP4D7 group (n = 81). Recipients in the iP4D4 and iP4‐D7 groups received an i.m. administration of 150 mg iP4, on D4 or D7 (D0 was the day of expected oestrus). On D7, all recipients were evaluated by transrectal ultrasonography and those that had a CL received a fresh or vitrified in vitro‐produced embryo. In Exp. 2 and 3, the CL area was also determined by ultrasonography at the time of FTET. The pregnancy diagnosis was performed at 30 days in Exp. 1, 57 days in Exp. 2, and between 40 and 72 days of pregnancy in Exp. 3. In Exp. 1, the pregnancy rate did not differ (p > .1) between the Control group (38.2% [34/89]) and iP4D4 group (49.5% [45/91]); yet, a parity effect indicated a greater (p < .05) pregnancy rate in heifers (57.9% [44/76]) than cows (30.8% [32/104]). In Exp. 2, the pregnancy rate was greater (p < .05) in the iP4D7 group (45.0% [65/144]) than in the Control group (34.0% [50/147]). Also, a greater (p = .08) pregnancy rate was observed for recipients with a small CL (≤2.75 cm2) that were treated with iP4 on the day of FTET than the control recipients (46.4% [32/69] vs. 32.6% [28/86]). In Exp. 3, no significant effects (p > .1) of the treatment group or CL size were detected on pregnancy rates at days 30 and 60. In conclusion, the beneficial effects of iP4 supplementation at early dioestrus on pregnancy maintenance may vary according to the experimental conditions, but its use at the time of FTET can be used as an alternative to enhance the fertility of beef recipients in challenging conditions in commercial herds.

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Supplementation with long-acting progesterone in early diestrus in beef cattle: I. effect of artificial insemination on onset of luteolysis

Cited by 9 publications

References 31 publications

Effects of Developmental Programming Caused by Maternal Nutrient Intake on Postnatal Performance of Beef Heifers and Their Calves

Effects of Developmental Programming Caused by Maternal Nutrient Intake on Postnatal Performance of Beef Heifers and Their Calves

Influence of Injectable Progesterone on the Pregnancy Rate and Gestational Loss of Nellore Females of Different Reproductive Categories Undergoing Fixed-Time Artificial Insemination

Effects of long‐acting injectable progesterone supplementation at early dioestrus on pregnancy maintenance in beef and dairy recipient cattle

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