Supplementing zinc oxide nanoparticles to cryopreservation medium minimizes the freeze-thaw-induced damage to spermatozoa

Isaac, Ann V.; Kumari, Sandhya; Nair, Ramya; Urs, Deepak Raj; Salian, Sujith Raj; Kalthur, Guruprasad; Adiga, Satish Kumar; Manikkath, Jyothsna; Mutalik, Srinivas; Sachdev, Divya; Pasricha, Renu

doi:10.1016/j.bbrc.2017.10.112

Cited by 80 publications

(57 citation statements)

References 28 publications

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“…Our findings agree with an earlier report by Isaac et al [79] who reported that ZnONPs have membrane protective function. ZnONPs supplementation leads to an increase in antioxidant enzymes and improves the quality of sperm [20,38,84,85].…”

Section: Discussionsupporting

confidence: 94%

“…The nanoparticles form ZnONPs thought to be had a more efficient interaction with protamines [79]. According to the TEM results of Isaac et al [79], the nanoparticles (ZnONPs) were accumulated around the spermatozoa and did not internalize into the sperm. A similar observation was recorded after using the fluorescent ZnONPs; the viable sperm membrane is not permeable to ZnONPs; while, the fluorescence ZnONPs was observed only in the dead spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

“…These factors give synthetic nanoparticles physicochemical features and a higher surface reactivity than their counterparts of the regular trace elements and vitamins [82,83]. The nanoparticles form ZnONPs thought to be had a more efficient interaction with protamines [79]. According to the TEM results of Isaac et al [79], the nanoparticles (ZnONPs) were accumulated around the spermatozoa and did not internalize into the sperm.…”

Section: Discussionmentioning

confidence: 99%

“…Nanotechnology, such as nano-zinc and nano-selenium, can be used to achieve bioactive properties in the reproduction, digestion, development, and freezing cells of various elements [78]. Recent studies have shown the beneficial effects of nanoparticles for sperm freezing in humans and animals by lowering the sperm chromatin damage and MDA levels [79][80][81]. However, no studies have examined the impact of zinc oxide or selenium nanoparticles on epididymal camel sperm.…”

Section: Discussionmentioning

confidence: 99%

“…The spermatozoa membrane has a net negative charge and the ZnONPs have a neutral charge. The ZnONPs cannot dissociate to ions which is probably the reason why they cannot enter into the sperm chromatin [79].…”

Section: Discussionmentioning

confidence: 99%

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Comparison between the Effects of Adding Vitamins, Trace Elements, and Nanoparticles to SHOTOR Extender on the Cryopreservation of Dromedary Camel Epididymal Spermatozoa

Shahin

Khalil

Saadeldin

et al. 2020

Animals

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There are several obstacles in camel semen cryopreservation; such as increasing semen viscosity and the reduction in motile spermatozoa after ejaculation. Epididymal spermatozoa offer an efficient alternative to overcome these problems and are well-suited for artificial insemination in camels. In the current study, we compared the effects of supplementation with vitamin C, E, inorganic trace elements of selenium (Na2SeO3) and zinc (ZnSO4), and zinc and selenium nanoparticles (ZnONPs and SeNPs, respectively) on the cryopreservation of dromedary camel epididymal spermatozoa. When the SHOTOR extender was supplemented with ZnONPs and SeNPs; the sperm showed increased progressive motility; vitality; and membrane integrity after cooling at 5 °C for 2 h; when compared to the control and vitamin-supplemented groups. Moreover, the ZnONPs and SeNPs supplementation improved the progressive motility, vitality, sperm membrane integrity, ultrastructural morphology, and decreased apoptosis when frozen and thawed. SeNPs significantly increased reduced glutathione (GSH), superoxide dismutase (SOD), and decreased lipid peroxide malondialdehyde (MDA) levels. The advantageous effects of the trace elements were potentiated by reduction into a nano-sized particle, which could increase bioavailability and reduce the undesired liberation of toxic concentrations. We recommend the inclusion of SeNPs or ZnONPs to SHOTOR extenders to improve the cryotolerance of camel epididymal spermatozoa.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Comparison between the Effects of Adding Vitamins, Trace Elements, and Nanoparticles to SHOTOR Extender on the Cryopreservation of Dromedary Camel Epididymal Spermatozoa

Shahin

Khalil

Saadeldin

et al. 2020

Animals

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Magnetic‐Assisted Control of Eggs and Embryos via Zona Pellucida‐Linked Nanoparticles

García‐Vázquez,

Garrappa,

Luongo

et al. 2024

Advanced Science

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Eggs and embryo manipulation is an important biotechnological challenge to enable positioning, entrapment, and selection of reproductive cells to advance into a new era of nature‐like assisted reproductive technologies. Oviductin (OVGP1) is an abundant protein in the oviduct that binds reversibly to the zona pellucida, an extracellular matrix that surrounds eggs and embryos. Here, the study reports a new method coupling OVGP1 to magnetic nanoparticles (NP) forming a complex (NPOv). NPOv specifically surrounds eggs and embryos in a reversible manner. Eggs/embryos bound to NPOv can be moved or retained when subjected to a magnetic force, and interestingly only mature‐competent eggs are attracted. This procedure is compatible with normal development following gametes function, in vitro fertilization, embryo development and resulting in the birth of healthy offspring. The results provide in vitro proof‐of‐concept that eggs and embryos can be precisely guided in the absence of physical contact by the use of magnets.

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Benefits and challenges of nanomaterials in assisted reproductive technologies

Silva

Barroso

Nascimento

et al. 2021

Molecular Reproduction Devel

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Assisted reproductive technology (ART) have contributed to preserve fertility in humans and to increase multiplication of genetically superior animals. Despite being highly practiced worldwide, ART presents some challenges, especially because gametes and embryos are kept in vitro for a variable period of time, and the oxidative stress in vitro can have negative impact on oocyte competence and embryo development. Nanotechnology needs to be considered to help overcome some of those impairments, since it can provide strategies to deliver antioxidants and hormones to gametes and embryos in vitro. The application of nanotechnology to ART can allow the development of new protocols using nanomaterials to improve in vitro oocyte competence and embryo production. This review discusses the applicability of nanomaterials to improve sperm selection, to deliver antioxidants and hormones to preantral follicles, oocytes, and embryos in vitro, as well as the concerns about using nanotechnology in ART.

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Supplementing zinc oxide nanoparticles to cryopreservation medium minimizes the freeze-thaw-induced damage to spermatozoa

Cited by 80 publications

References 28 publications

Comparison between the Effects of Adding Vitamins, Trace Elements, and Nanoparticles to SHOTOR Extender on the Cryopreservation of Dromedary Camel Epididymal Spermatozoa

Comparison between the Effects of Adding Vitamins, Trace Elements, and Nanoparticles to SHOTOR Extender on the Cryopreservation of Dromedary Camel Epididymal Spermatozoa

Magnetic‐Assisted Control of Eggs and Embryos via Zona Pellucida‐Linked Nanoparticles

Benefits and challenges of nanomaterials in assisted reproductive technologies

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