In today’s increasingly globalizing world, the demand for the transport of more and more specific kinds of goods is constantly growing. Greater diversity of the objects of transport extends the range of consignees and consignors, which implies the reduction of the number and volume of consignments. Modern enterprises are no longer interested in mass supplies of large quantities of the same material but strive for flexible supplies of smaller quantities of specific materials out of more suppliers. This manuscript deals with the streamlining of specific transport-logistics processes, including a detailed analysis of the existing pick-up system in relation to transport serviceability of the certain territory starting in the city of České Budějovice (Czech Republic) and a determination of more effective variants for supply. The primary objective will be to find and analyze specific options for streamlining the processes being optimized using the selected method of the graph theory, namely the location problem. In the context of optimization, a specific case study is elaborated with the aim to increase the efficiency of the current pickup system, i.e. serviceability of a particular territory.