Supported lipid bilayers using extracted microbial lipids: domain redistribution in the presence of fengycin

Mantil, Elisabeth; Crippin, Trinda; Avis, Tyler J.

doi:10.1016/j.colsurfb.2019.02.050

Cited by 16 publications

(5 citation statements)

References 50 publications

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“…These data are consistent to that of Mantil et al . 21 , which showed that the microbial sensitivity to FE correlates with a greater fluidity of membranes of target microorganisms compared to those of tolerant microorganisms.…”

Section: Resultsmentioning

confidence: 99%

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Fengycin induces ion channels in lipid bilayers mimicking target fungal cell membranes

Zakharova

Efimova

Malev

et al. 2019

Sci Rep

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The one-sided addition of fengycin (FE) to planar lipid bilayers mimicking target fungal cell membranes up to 0.1 to 0.5 μM in the membrane bathing solution leads to the formation of well-defined and well-reproducible single-ion channels of various conductances in the picosiemens range. FE channels were characterized by asymmetric conductance-voltage characteristic. Membranes treated with FE showed nonideal cationic selectivity in potassium chloride bathing solutions. The membrane conductance induced by FE increased with the second power of the lipopeptide aqueous concentration, suggesting that at least FE dimers are involved in the formation of conductive subunits. The pore formation ability of FE was not distinctly affected by the molecular shape of membrane lipids but strongly depended on the presence of negatively charged species in the bilayer. FE channels were characterized by weakly pronounced voltage gating. Small molecules known to modify the transmembrane distribution of electrical potential and the lateral pressure profile were used to modulate the channel-forming activity of FE. The observed effects of membrane modifiers were attributed to changes in lipid packing and lipopeptide oligomerization in the membrane.

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Section: Resultsmentioning

confidence: 99%

“…Recently, Mantil et al . 21 showed that FE activity suppression by ERG might be related to the inhibition of the ordering effect of lipopeptide by sterol. Using molecular dynamics simulation, Sur et al .…”

Section: Introductionmentioning

confidence: 99%

Fengycin induces ion channels in lipid bilayers mimicking target fungal cell membranes

Zakharova

Efimova

Malev

et al. 2019

Sci Rep

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“… Wójtowicz et al (2021) demonstrated that surfactin targets raft domains. Fengycin and polymyxin B were shown to modulate the phase separation and domain organization of microbial lipids ( Mantil et al, 2019 ; Fu et al, 2022 ). Mycosubtilin induced changes in the organization and morphology of cholesterol- and sphingomyelin-enriched membranes ( Nasir and Besson, 2011 ).…”

Section: Resultsmentioning

confidence: 99%

Cyclic lipopeptides as membrane fusion inhibitors against SARS-CoV-2: New tricks for old dogs

et al. 2023

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“…The present work focused on the affects of the presence or absence of cholesterol, but going forward with ergosterol is the next logical step, especially the recent work showing that ergosterol also modulates fengycin function. (49,50) However, in the context of the MARTINI model cholesterol and ergosterol are very similar, so much so that distinguishing their effects appeared unlikely to succeed. We suspect that repeating these calculations with fully atomistic methods will be necessary.…”

Section: Discussionmentioning

confidence: 99%

Effects of Cholesterol on the mechanism of fengycin, a biofungicide

Sur

Grossfield

2021

Preprint

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Fengycins are a class of antifungal lipopeptides synthesized by the bacteria Bacillus subtilis, commercially available as the primary component of the agricultural fungicide Serenade. They are toxic to fungi, but far less to mammalian cells. One key difference between mammalian and fungal cell membranes is the presence of cholesterol only in the former; recent experimental work showed that the presence of cholesterol reduces fengycin-induced membrane leakage. Since our previous all-atom and coarse-grained simulations suggested that aggregation of membrane-bound fengycin is central to its ability to disrupt membranes, we hypothesized that cholesterol might reduce fengycin aggregation. Here, we test this hypothesis using coarse-grained molecular dynamics simulations, with sampling enhanced via the weighted ensemble method. The results indicate that cholesterol subtly alters the size distribution for fengycin aggregates, limits the lateral range of their membrane disordering, and reduces the ability of aggregates to bend the membrane. Taken together, these phenomena may account for cholesterols' affects on fengycin activity.

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Supported lipid bilayers using extracted microbial lipids: domain redistribution in the presence of fengycin

Cited by 16 publications

References 50 publications

Fengycin induces ion channels in lipid bilayers mimicking target fungal cell membranes

Fengycin induces ion channels in lipid bilayers mimicking target fungal cell membranes

Cyclic lipopeptides as membrane fusion inhibitors against SARS-CoV-2: New tricks for old dogs

Effects of Cholesterol on the mechanism of fengycin, a biofungicide

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