Supported Liquid Extraction (SLE) in LC‐MS Bioanalysis

Cheng, Zhongzhe; Jiang, Hongliang

doi:10.1002/9781119274315.ch6

Cited by 6 publications

(3 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A sample volume of 100 μl was selected to minimise the amount of plasma required while maintaining our target sensitivity (≤5 ng/ml LLOQ for all analytes). SLE was performed semi‐autonomously using a Biotage (Uppsala, Sweden) Extrahera extraction automation system 22 . SLE was assessed with and without a preceding protein precipitation step.…”

Section: Methodsmentioning

confidence: 99%

“…SLE was performed semi-autonomously using a Biotage (Uppsala, Sweden) Extrahera extraction automation system. 22 SLE was assessed with and without a preceding protein precipitation step. When it became apparent that a protein precipitation step was advantageous, several reconstitution solvents for the subsequent SLE were trialled (0.1% formic acid in water, 0.1% ammonium formate in water, and with and without 90-μl acetonitrile).…”

Section: Optimization Of Sample Preparation and Extractionmentioning

confidence: 99%

See 1 more Smart Citation

A validated method for the simultaneous quantification of cannabidiol, Δ⁹‐tetrahydrocannabinol, and their metabolites in human plasma and application to plasma samples from an oral cannabidiol open‐label trial

Kevin

Vogel

Doohan

et al. 2020

Drug Testing and Analysis

View full text Add to dashboard Cite

Cannabidiol (CBD) and Δ9‐tetrahydrocannabinol (THC) are the two best known and most extensively studied phytocannabinoids within Cannabis sativa. An increasing number of preclinical studies and clinical trials have been conducted with one or both compounds, often probing their therapeutic effects in conditions such as paediatric epilepsy, anxiety disorders or chronic pain. Accurate monitoring of THC and CBD and their metabolites is essential for tracking treatment adherence and pharmacokinetics. However, fully validated methods for the comprehensive analysis of major Phase I CBD metabolites are yet to be developed due to a historical lack of commercially available reference material. In the present study, we developed, optimised and validated a method for the simultaneous quantification of CBD, THC and their major Phase I metabolites 6‐hydroxy‐CBD (6‐OH‐CBD), 7‐hydroxy‐CBD (7‐OH‐CBD), 7‐carboxy‐CBD (7‐COOH‐CBD), 11‐hydroxy‐tetrahydrocannabinol (11‐OH‐THC) and 11‐carboxy‐tetrahydrocannabinol (11‐COOH‐THC) as per Food and Drug Administration (FDA) guidelines for bioanalytical method validation. The method is accurate, reproducible, sensitive and can be carried out in high‐throughput 96‐well formats, ideal for larger scale clinical trials. Deuterated internal standards for each analyte were crucial to account for variable matrix effects between plasma lots. The application of the method to plasma samples, taken from people who had been administered oral CBD as part of an open‐label trial of CBD effects in anxiety disorders, demonstrated its immediate utility in ongoing and upcoming clinical trials. The method will prove useful for future studies involving CBD and/or THC and can likely accommodate the inclusion of additional metabolites as analytical reference materials become commercially available.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Optimization Of Sample Preparation and Extractionmentioning

confidence: 99%

A validated method for the simultaneous quantification of cannabidiol, Δ⁹‐tetrahydrocannabinol, and their metabolites in human plasma and application to plasma samples from an oral cannabidiol open‐label trial

Kevin

Vogel

Doohan

et al. 2020

Drug Testing and Analysis

View full text Add to dashboard Cite

show abstract

“…Mechanical stirring agitates the batch volume and moves the material into the cavitation zone. This accelerates the mass transfer of the extract, increasing the permeability of cell walls and producing cavitation [38]. Large-scale applications are limited due to the higher cost and undesirable changes in active constituents, particularly at energies higher than 20 kHz.…”

Section: Ultrasonic Extraction (Use)mentioning

confidence: 99%

Generalized Review on Extraction of Biomolecules

Shireen¹,

Zarena²

2022

J. Pharm. Nutr. Sci.

View full text Add to dashboard Cite

Nature has given us a wide range of biological compounds that can be utilized to help combat health problems, but sometimes with over-processing, these advantages are diminished or lost. Food and pharmaceutical companies have developed a range of new approaches to harness and retain the naturally occurring diversity and quality of bioactive compounds efficiently and effectively. Pharmaceutically important plant products have been known for millennia; they have been used in crude and unrefined forms. One of the best ways to pick the best plant bioactive is through genetic engineering, omics, and plant tissue culture. Many laboratories routinely screen plant species for bioactive compounds to discover new ones. All extraction methods depend on the researcher's preference and what exactly the research entails. Successful extraction begins with the careful selection and preparation of plant samples and thorough knowledge and review of the appropriate literature. Here we have attempted to describe the different stages and methods of extraction from the medicinal plants. From the review, it can be concluded that no universal extraction method is ideal and that each extraction procedure is unique.

show abstract

Chromatographic bioanalytical assays for targeted covalent kinase inhibitors and their metabolites

Retmana

Beijnen

Sparidans

2021

Journal of Chromatography B

View full text Add to dashboard Cite

Supported Liquid Extraction (SLE) in LC‐MS Bioanalysis

Cited by 6 publications

References 42 publications

A validated method for the simultaneous quantification of cannabidiol, Δ⁹‐tetrahydrocannabinol, and their metabolites in human plasma and application to plasma samples from an oral cannabidiol open‐label trial

A validated method for the simultaneous quantification of cannabidiol, Δ⁹‐tetrahydrocannabinol, and their metabolites in human plasma and application to plasma samples from an oral cannabidiol open‐label trial

Generalized Review on Extraction of Biomolecules

Chromatographic bioanalytical assays for targeted covalent kinase inhibitors and their metabolites

Contact Info

Product

Resources

About

Supported Liquid Extraction (SLE) in LC‐MS Bioanalysis

Cited by 6 publications

References 42 publications

A validated method for the simultaneous quantification of cannabidiol, Δ9‐tetrahydrocannabinol, and their metabolites in human plasma and application to plasma samples from an oral cannabidiol open‐label trial

A validated method for the simultaneous quantification of cannabidiol, Δ9‐tetrahydrocannabinol, and their metabolites in human plasma and application to plasma samples from an oral cannabidiol open‐label trial

Generalized Review on Extraction of Biomolecules

Chromatographic bioanalytical assays for targeted covalent kinase inhibitors and their metabolites

Contact Info

Product

Resources

About

A validated method for the simultaneous quantification of cannabidiol, Δ⁹‐tetrahydrocannabinol, and their metabolites in human plasma and application to plasma samples from an oral cannabidiol open‐label trial

A validated method for the simultaneous quantification of cannabidiol, Δ⁹‐tetrahydrocannabinol, and their metabolites in human plasma and application to plasma samples from an oral cannabidiol open‐label trial