The increase in the number of users on wireless local area networks (WLAN) and the development of large size applications have increased the demand for high-speed data rate and low latency. The IEEE 802.11ac was developed to provide very high throughput WLANs. Many enhancements are added to the medium access control (MAC) and physical (PHY) layers to increase data rate and improve network performance, these features enable the IEEE 802.11ac standard to provide quality of service (QoS) for multimedia applications. This paper concentrates on the impact of QoS on the system performance in term of delay and throughput. Four scenarios are proposed to investigate the network performance with different (from 1 up to 8) spatial stream (SS). The objective modular network testbed in C++ (OMNet++ modeler v5.5.1) is used to simulate and model these scenarios. For 8×8 SS, the results of simulation show the best throughput (maximum) and delay (minimum) values of (622, 484, 399.3, 382.96 Mbps) and (0.0211, 0.0589, 0.1037, 0.1202 sec) for 5, 15, 30 and 45 node number scenarios respectively. Although the number of nodes increases, the system performance decreases, however when QoS is deployed the performance is enhanced and its best improvement is obtained at the highest (45) node number scenario with values of 94.4% and 56.1% for throughput and delay respectively.