Supporting the scientific advancement from pathogenic microorganisms biobank

Kozlakidis, Zisis; Cheong, Io Hong; Wei, Qiang

doi:10.1016/j.bsheal.2022.09.002

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…This approach opens new ideas for the utilization of pathogenic resource centers. Moreover, the resource center provides a basis for research in various fields [ 33 , 34 ].…”

Section: Discussionmentioning

confidence: 99%

Screening of Staphylococcus aureus for Disinfection Evaluation and Transcriptome Analysis of High Tolerance to Chlorine-Containing Disinfectants

Song

Huang

et al. 2023

Microorganisms

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The nonstandard use of disinfectants can lead to the disinfectant resistance of bacteria and even increase antibiotic resistance. However, compared with the study of antibiotic resistance, studies of bacterial resistance to disinfectants are relatively few in number. In this study, we explored the standard strain screening procedure for the evaluation of disinfection efficacy. Staphylococcus aureus strains with different sources and substrates were selected from the National Pathogen Resource Center of China and screened the standard strains that could evaluate the long-term bacteriostatic effect of the chlorine-containing disinfectants through the determination of the physical properties, genome-based safety evaluation, and disinfection test evaluation. In this process, one S. aureus strain was more resistant to the long-term bacteriostasis of chlorine-containing disinfectants than the other strains. This strain and the standard strain ATCC 6538 were cultured in the medium containing a low concentration of chlorine-containing disinfectant synchronously. Then, comparative transcriptome analysis was carried out to investigate the potential mechanism of a high tolerance to chlorine-containing disinfectants. The pathway of significant differential expression is related to the oxocarboxylic acid metabolic mechanism, amino acid metabolic mechanism, and pyrimidine mechanism, which may be the molecular mechanism of S. aureus evolution to adapt to chlorine-containing disinfectants. Our study established a technical process for screening and evaluating standard strains for disinfection, which also provided a reference for studying the bacterial evolution mechanism toward chlorine tolerance.

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“…This approach opens new ideas for the utilization of pathogenic resource centers. Moreover, the resource center provides a basis for research in various fields [ 33 , 34 ].…”

Section: Discussionmentioning

confidence: 99%