Suppressed DNA base pair stacking assembly of gold nanoparticles in an alcoholic solvent for enhanced ochratoxin A detection in Baijiu

Abstract

The currently established DNA nanoprobes for detection of mycotoxin from beverages have been limited by complicated sample pretreatment and uncontrollable nanoparticle flocculation in complex systems. We develop a rapid colorimetric...

Naked-Eye LAMP Assay of M. tuberculosis in Sputum by In Situ Au Nanoprobe Identification: For the In Vitro Diagnostics of Tuberculosis

“…The resultant extinction spectra of the DNA-AuNPs revealed a plasmonic red-shift of about 2 nm for each reaction ratio, compared with that of the AuNPs (Figure b). In previous works, similar results were reported. , …”

“…tuberculosis from both sample 1′ and sample 4′ (Figure b). It is therefore deduced that the naked-eye LAMP assay demonstrated in Figure possesses higher accuracy (96.2%) and sensitivity (92.3%) than the mostly adopted PCR (92.3 and 84.6%, respectively) (Figure b) . In addition, the present approach is advantageous over the conventional testing methods in terms of rapidity and low cost (Table ), including the approach of cultures and T-SPOT.TB.…”

“…It is therefore deduced that the naked-eye LAMP assay demonstrated in Figure 5 possesses higher accuracy (96.2%) and sensitivity (92.3%) than the mostly adopted PCR (92.3 and 84.6%, respectively) (Figure 6b). 31 In addition, the present approach is advantageous over the conventional testing methods in terms of rapidity and low cost (Table 2), including the approach of cultures and T-SPOT.TB. Unlike smear microscopy and PCR methods that require precise equipment and professional background, only a common water bath or a temperature controller is needed to perform the naked-eye LAMP assay.…”

“…In previous works, similar results were reported. 30,31 The system containing the LAMP template can be readily discriminated in color from that of the negative control upon the addition of MgCl 2 when the reaction ratio was in the range of 200:1 and 800:1. As depicted in Figure 3d, the DNA-AuNPs without the plasmid turned gray due to salt-induced aggregation, while the DNA-AuNPs remained considerably stabilized in the presence of the plasmid against aggregation due to the steric effect upon binding with the amplicon.…”

Nanoscale Materials Applying for the Detection of Mycotoxins in Foods

“…As the degree of NPs’ aggregation increased, the absorption wavelength changed, resulting in a blue-colored solution. Based on this principle, rapid detection methods for AFB 2 , OTA, and AFB 1 were developed [ 185 , 186 ]. Mehdi Dadmehr and co-workers utilized carboxyfluorescein (FAM) tags to label specific aptamers with internal complementary sequences [ 187 ].…”