Suppressed expression of genes involved in transcription and translation in in vitro compared with in vivo cultured bovine embryos

Corcoran, D; Fair, Trudee; Park, S.; Rizos, Dimitrios; Patel, Osman V.; Smith, George W.; Coussens, Paul M.; Ireland, James J.; Boland, M.P.; Evans, A.C.O.; Lonergan, P.

doi:10.1530/rep.1.01015

Cited by 108 publications

(76 citation statements)

References 60 publications

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“…We both confirmed previously detected transcripts such as IGF2R and DNMT1 and identified previously uncharacterized transcripts such as regulator of chromatin-structure SWI/ SNF and NFYA at the 8-cell stage (Table 2). In vitro-produced bovine blastocysts are known to have different mRNA expression patterns as compared with their in vivo-derived counterparts (14,15). Although lower than in vivo-derived blastocysts, reasonable pregnancy and calving rates (35-45%) have been obtained by using in vitro-derived bovine blastocysts (7,15).…”

Section: Discussionmentioning

confidence: 99%

“…In vitro-produced bovine blastocysts are known to have different mRNA expression patterns as compared with their in vivo-derived counterparts (14,15). Although lower than in vivo-derived blastocysts, reasonable pregnancy and calving rates (35-45%) have been obtained by using in vitro-derived bovine blastocysts (7,15). It might be that the in vitro-cultured embryos express different transcripts to deal with the suboptimal culture conditions and that the expression profiles do not necessarily indicate their abnormality.…”

Section: Discussionmentioning

confidence: 99%

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Dynamics of global transcriptome in bovine matured oocytes and preimplantation embryos

Mısırlıoğlu

Page²,

Sağırkaya³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

141

106

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Global activation of the embryonic genome is the most critical event in early mammalian development. After fertilization, a rich supply of maternal proteins and RNAs support development whereas a number of zygotic and embryonic genes are expressed in a stage-specific manner leading to embryonic genome activation (EGA). However, the identities of embryonic genes expressed and the mechanism(s) of EGA are poorly defined in the bovine. Using the Affymetrix bovine-specific DNA microarray as the biggest available array at present, we analyzed gene expression at two key stages of bovine development, matured oocytes (MII) and 8-cell-stage embryos, constituting the ultimate reservoir for life and a stage during which EGA takes place, respectively. Key genes in regulation of transcription, chromatin-structure cell adhesion, and signal transduction were up-regulated at the 8-cell stage as compared with 8-cell embryos treated with ␣-amanitin and MII. Genes controlling DNA methylation and metabolism were upregulated in MII. These changes in gene expression, related to transcriptional machinery, chromatin structure, and the other cellular functions occurring during several cleavage stages, are expected to result in a unique chromatin structure capable of maintaining totipotency during embryogenesis and leading to differentiation during postimplantation development. Dramatic reprogramming of gene expression at the onset of development also has implications for cell plasticity in somatic cell nuclear transfer, genomic imprinting, and cancer.gene expression ͉ microarray

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Dynamics of global transcriptome in bovine matured oocytes and preimplantation embryos

Mısırlıoğlu

Page²,

Sağırkaya³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

141

106

View full text Add to dashboard Cite

show abstract

“…Up-regulated genes in NT embryos were suggested to compromise membrane formation (nidogen 2) and increase maternal immune responses to over-expressed MHC-I genes. Corcoran et al (2006) identified differences in gene expression profiles between bovine blastocysts derived from in vivo v. in vitro. Of the 384 genes and expressed sequence tags that were differentially expressed, 85% were down-regulated in the latter.…”

Section: Recent Transcriptomic Studies On Dairy Cow Fertilitymentioning

confidence: 99%

“…Of the 384 genes and expressed sequence tags that were differentially expressed, 85% were down-regulated in the latter. The inferior quality of in vitroderived embryos was associated with a decreased level of gene expression and, possibly, caused by a deficiency of the machinery associated with transcription and translation (Corcoran et al, 2006). Gene expression profiles can be used to define normal and deviant embryos and from here optimize embryo production protocols.…”

Section: Recent Transcriptomic Studies On Dairy Cow Fertilitymentioning

confidence: 99%

Expression profiles of genes regulating dairy cow fertility: recent findings, ongoing activities and future possibilities

Beerda

Wyszyńska-Koko

Pas

et al. 2008

Animal

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Subfertility has negative effects for dairy farm profitability, animal welfare and sustainability of animal production. Increasing herd sizes and economic pressures restrict the amount of time that farmers can spend on counteractive management. Genetic improvement will become increasingly important to restore reproductive performance. Complementary to traditional breeding value estimation procedures, genomic selection based on genome-wide information will become more widely applied. Functional genomics, including transcriptomics (gene expression profiling), produces the information to understand the consequences of selection as it helps to unravel physiological mechanisms underlying female fertility traits. Insight into the latter is needed to develop new effective management strategies to combat subfertility. Here, the importance of functional genomics for dairy cow reproduction so far and in the near future is evaluated. Recent gene profiling studies in the field of dairy cow fertility are reviewed and new data are presented on genes that are expressed in the brains of dairy cows and that are involved in dairy cow oestrus (behaviour). Fast-developing new research areas in the field of functional genomics, such as epigenetics, RNA interference, variable copy numbers and nutrigenomics, are discussed including their promising future value for dairy cow fertility.

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“…Several major developmental events occur during this period, including the first cleavage division, the timing of which is important in determining subsequent embryo development; embryonic genome activation and morula compaction which involves establishment of the first intimate cell-to-cell contact in the embryo and blastocyst formation involving differentiation of two cell types, the trophectoderm and inner cell mass (Bavister 1995;Lonergan et al 1999). Many of the differences in embryo quality can be attributed to culture environment medium-induced changes in mRNA abundance (Wrenzycki et al 1996;Corcoran et al 2006). Studies on the embryonic genome indicate that currently used in vitro embryo development conditions cannot fully mimic in vivo conditions with regard to mRNA expression.…”

Section: Introductionmentioning

confidence: 99%

Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

et al. 2016

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The objective of this study was to compare effects of in vitro culture systems on embryonic development and expression patterns of developmentally important genes in preimplantation buffalo embryos. After IVM/IVF presumptive zygotes were cultured in one of three systems: undefined TCM-199, mCR2aa medium supplemented with 10 % FBS and defined PVA-myo-inositol-phosphate-EGF medium. No (P [ 0.05) differences at 2-cell, 4-cell and 8-cell to 16-cell stages were observed among the three cultured media used, however, increased (P \ 0.05) blastocyst yield, cell number and hatching rate were found in defined medium compared to undefined media. The expression patterns of genes implicated in embryo metabolism (GLUT-1), anti-apoptosis (BCL-2), imprinting (IGF-2R), DNA methylation (DNMT-3A) and maternal recognition of pregnancy (IFNT) were increased (P \ 0.05) in hatched blastocysts derived from defined medium compared to undefined media. In conclusion, serum-free, defined medium improved developmental competence of in vitro cultured buffalo embryos. Whether these differences in morphological development and gene expression have long-term effects on buffalo calves born after embryo transfer remains unknown. However, it is possible that early adaptations of the preimplantation embryo to its environment persist during fetal and post-natal development.

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Suppressed expression of genes involved in transcription and translation in in vitro compared with in vivo cultured bovine embryos

Cited by 108 publications

References 60 publications

Dynamics of global transcriptome in bovine matured oocytes and preimplantation embryos

Dynamics of global transcriptome in bovine matured oocytes and preimplantation embryos

Expression profiles of genes regulating dairy cow fertility: recent findings, ongoing activities and future possibilities

Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

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