Suppressed proliferation of mouse osteoblast-like cells by a rough-surfaced substrate leads to low differentiation and mineralization

Saito, Takao; Hayashi, Hikoshiro; Kameyama, Tetsuya; Hishida, Masako; Nagai, Kazuo; Teraoka, Kay; Kato, Katsuya

doi:10.1016/j.msec.2009.06.010

Cited by 34 publications

(28 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cells were maintained and grown in Minimum Essential Medium-α (α-MEM, Life Technologies, CA) containing 10% fetal bovine serum and antibiotics in a humidified 5% CO 2 balanced-air incubator at 37°C [10]. The differentiation medium was prepared by adding 50 μg/ml ascorbic acid and 10 mM β-glycerophosphate to the growth medium.…”

Section: Cell Culturementioning

confidence: 99%

“…From previous results on the effects of surface roughness on the cell adhesion, growth, and differentiation of MC3T3-E1 cells [10], we assumed that cell growth and function could be controlled by structured surfaces at the micrometer scale. Based on this assumption regarding cell-surface interactions, in the present study we focused on how the geometry and dimensions of 3-D structured surfaces affect cell behavior.…”

Section: Fabrication and Characterization Of The 3-d Structuresmentioning

confidence: 99%

“…The topography at the nanometer scale affects sub-cellular behavior such as the organization of cell adhesion molecule receptors, whereas at the micrometer level, cellular and supra-cellular characteristics such as cell morphology and migration are influenced [10,12,18]. For instance, a previous study by Chen et al [19] demonstrated using capillary endothelial cells that cell shape is the critical determinant that governs whether individual cells grow or die.…”

Section: Cell Morphologymentioning

confidence: 99%

“…To investigate cellular responses to physically structured surfaces, the effects of sub-micrometer-and micrometer-scaled surface roughness were previously examined along with the sequential events of cell adhesion, growth, differentiation, and mineralization [10]. The …”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Arrayed three-dimensional structures designed to induce and maintain a cell pattern by a topographical effect on cell behavior

Saito

Teraoka

Ota

2015

Materials Science and Engineering: C

View full text Add to dashboard Cite

Section: Cell Culturementioning

confidence: 99%

Section: Fabrication and Characterization Of The 3-d Structuresmentioning

confidence: 99%

Section: Cell Morphologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Arrayed three-dimensional structures designed to induce and maintain a cell pattern by a topographical effect on cell behavior

Saito

Teraoka

Ota

2015

Materials Science and Engineering: C

View full text Add to dashboard Cite

“…In the past decades, a plethora of data on the roles of mechanical and structural cues in modulating cellular behaviors has emerged (1)(2)(3)(4)(5). It is increasingly evident that cell fates can be changed by engineering the physical properties of the microenvironment to which the cells are exposed (6 -8).…”

mentioning

confidence: 99%

Biochemical Characterization of the Cell-Biomaterial Interface by Quantitative Proteomics

Tong

Liang

Tam

et al. 2010

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

The growth and differentiation of cells in multicellular organisms are regulated by the complex interplay of biochemical and mechanical signals. In the past decades, a plethora of data on the roles of mechanical and structural cues in modulating cellular behaviors has emerged (1-5). It is increasingly evident that cell fates can be changed by engineering the physical properties of the microenvironment to which the cells are exposed (6 -8). These observations have inspired the development of functionalized biomaterials that can directly and specifically interact with tissue components, and support or even direct the appropriate cellular activities (9, 10). Although promising progress has been observed in the past few years, several gaps in knowledge in this field have hindered the development of such "intelligent" biomaterials. In particular, the understanding of the mechanism in which the cell orchestrates physiological and morphological changes by translating mechanical and structural information into biochemical signals is still very limited.As a standard experimental model, cell lines cultured in vitro as a monolayer over solid substrata are usually used to study the effects of biomaterial surfaces on cellular phenotypes. With this simple model system, ingenious experiments have shown that physical forces applied through the extracellular matrix (ECM) 1 1 can induce changes in cell adhesion molecules and stress-induced ion channels, which then lead to changes in the cytoskeleton and gene expressions (11-13). We term the biochemical structure present at the interface between the substratum and the cellular interior the adherence surface (AS), which is composed of the basal plasma membrane with associated structures such as the ECM on one side and the focal adherence complexes on the other. In monolayer cell culture systems, the AS is the only part of the cells in direct contact with the substratum, and is therefore responsible for the first line of communication between the cells and the biomaterial. It is likely that the AS is the organelle that mediates the communication of mechanical and tectonic signals from the substratum to biochemical transducers in the cells. Given the complexity of this process, it is clear that the understanding of this phenomenon cannot be achieved merely by studying individual biological parts in isolation. It is necessary, therefore, to systematically characterize the biochemical factors that mediate the interactions between cells and materials to yield insights into intracellular signaling processes that are responsible for such cellular responses. Toward this goal, we seek to investigate the biochemical basis of how different biomaterials may impose changes in the composition of the AS of adherent cells.

show abstract

Expression of Mineralized Tissue‐Associated Proteins is Highly Upregulated in MC3T3‐E1 Osteoblasts Grown on a Borosilicate Glass Substrate

Jain

Marzillier

Kowal

et al. 2011

Advances in Bioceramics and Porous Ceramics IV

View full text Add to dashboard Cite

Suppressed proliferation of mouse osteoblast-like cells by a rough-surfaced substrate leads to low differentiation and mineralization

Cited by 34 publications

References 25 publications

Arrayed three-dimensional structures designed to induce and maintain a cell pattern by a topographical effect on cell behavior

Arrayed three-dimensional structures designed to induce and maintain a cell pattern by a topographical effect on cell behavior

Biochemical Characterization of the Cell-Biomaterial Interface by Quantitative Proteomics

Expression of Mineralized Tissue‐Associated Proteins is Highly Upregulated in MC3T3‐E1 Osteoblasts Grown on a Borosilicate Glass Substrate

Contact Info

Product

Resources

About