Suppression effect of LHRH-A and hCG on Piwi expression in testis of Nile tilapia Oreochromis niloticus

Xiao, Jun; Zhou, Yi; Luo, Yang; Zhong, Huan; Huang, Yulin; Zhang, Yan; Luo, Zujuan; Ling, Zhengbao; Zhang, Ming; Xi, Gan

doi:10.1016/j.ygcen.2013.04.021

Cited by 21 publications

(16 citation statements)

References 30 publications

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“…In Nile tilapia, to date there has been identified four nanos genes (Bellaiche et al, 2014;Škugor et al, 2014a), two piwil genes (Xiao et al, 2013) and three vasa (Conte et al, 2017;Fujimura et al, 2011). Further in silico analysis of public databases revealed predicted sequences for one dnd1 as well as three pum genes.…”

Section: Introductionmentioning

confidence: 99%

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Expression pattern of nanos, piwil, dnd, vasa and pum genes during ontogenic development in Nile tilapia Oreochromis niloticus

Jin

Davie

Migaud

2019

Gene

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Primordial germ cells (PGCs) are specified by maternally provided determinants in fish. PGCs migrate then into prospective gonadal sites during early development and give rise to germ cell lineage. PGC disrupted animals do not sexually mature which has a range of commercial as well as environmental benefits. To find potential target genes for sterilisation of Nile tilapia, relative mRNA abundance patterns and tissue distribution of four nanos, two piwil, dnd1, vasa and three pum genes were investigated during ontogenic development from unfertilised eggs to newly hatched larvae and in adult tissues, respectively. The ontogenic pattern of RNA abundance revealed that all the investigated gene transcripts are maternally deposited to varying degrees, except for nanos2 which is not expressed in eggs. The ontogenic patterns of relative RNA abundance could be grouped into three categories. The first one, including nanos3, piwil1, piwil2, dnd1 and vasa, showed abundant transcript levels during early developmental stages which are then degraded during the period of maternal to zygotic transition between blastula and gastrula stages with a reduction in expression of four to five orders of magnitude by hatching stage. Another, including pum2 and pum3, showed similar patterns to the first group, but the transcript levels are reduced by only two orders of magnitude. The third group, including nanos1a, nanos1b and pum1, was characterised by a zygotic increase. nanos2 had no detectable transcripts until hatching stage. The tissue screening of nanos1a, nanos1b, pum1, pum2 and pum3 showed that they are expressed in various tissues, implying their potential pleiotropic effects in these tissues apart from gonads. In contrast, nanos3, piwil1, piwil2, dnd1 and vasa appeared to be exclusively expressed in gonads (both ovary and testis), and nanos2 showed testis-specific expression. Based on these results nanos3, piwil1, piwil2, dnd1 and vasa were prioritised amongst the 11 selected genes as potential target genes for sterilisation in Nile tilapia as they have no significant zygotic expression during embryogenesis, they are expressed exclusively in gonads and maternally deposited. These features suggest a potential role of these genes in the specification and maintenance of PGCs during the ontogenic development of Nile tilapia.

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Section: Introductionmentioning

confidence: 99%

“…In Nile tilapia, there are also two piwi-like genes (piwil1 and piwil2); both proteins are present in spermatocytes but not in mature sperm (Xiao et al, 2013). Despite the apparent importance of Piwi family members in germline cells in diverse species, little is known about their molecular characteristics in fish.…”

Section: Introductionmentioning

confidence: 99%

Expression pattern of nanos, piwil, dnd, vasa and pum genes during ontogenic development in Nile tilapia Oreochromis niloticus

Jin

Davie

Migaud

2019

Gene

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“…In contrast to previous work performed in tilapia (Nakamura et al, 2015;Pandit et al 2015), this study examined the impact of temperature-induced germ cell loss at the molecular level. Both piwil1 and piwil2 have previously been reported to be expressed in various phases of testicular germ cells except for mature sperm in Nile tilapia (Xiao et al, 2013). In CT fish, both piwil1 and piwil2 expression levels gradually increased during the study in line with the progression of spermatogenesis.…”

Section: Discussionmentioning

confidence: 59%

“…Unlike piwil1 and piwil2 which are expressed in various phases of testicular germ cells (Xiao et al, 2013), nanos2, nanos3 and gfra1 are known as SSC-specific markers in mammals and fish (Bellaiche et al, 2014;Suzuki et al, 2009). The expression patterns of nanos2, nanos3 and gfra1 in CT fish were not significantly altered during the study period, implying that there was no significant change in the number of SSC and/or spermatogonia during this period.…”

Section: Discussionmentioning

confidence: 92%

“…In Nile tilapia, piwil1 and piwil2 transcripts are maternally deposited in eggs and exclusively expressed in both ovary and testis (Jin et al, 2019). In this species, both Piwil1 and Piwil2 proteins were present in various phases of testicular germ cells from spermatogonia to spermatid but not in spermatozoa (Xiao et al, 2013). nanos2 and nanos3 are RNA-binding zinc finger proteins and nanos2 is known to be a key regulator for the maintenance and modulation of spermatogonial stem cells (SSCs) self-renewal (Hofmann, 2008;Sada et al, 2009;Suzuki et al, 2009).…”

Section: Introductionmentioning

confidence: 98%

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Temperature-induced testicular germ cell loss and recovery in Nile tilapia Oreochromis niloticus

Jin

Davie

Migaud

2019

General and Comparative Endocrinology

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Water temperature is a critical external factor influencing gonadal development in fish. This research aimed to study the impact of elevated temperature on testicular germ cell survival and reproductive capacity of Nile tilapia. Male Nile tilapia were exposed to high temperatures of either 36 (HT1) or 37°C (HT2) for 3000 degree-days (DD) and thereafter returned to the control temperature of 27°C (CT) for 2200 DD. The deleterious effects on testicular germ and somatic cells were observed histologically, characterised by vacuolisation, atrophy and the loss of spermatogenic cells in testes with a more severe impact of HT2 compared to HT1. Interestingly, serum 11-ketotestosterone (11-KT) and testosterone (T) levels tended to be higher during the heat treatments than CT. Expression levels of germline-specific genes piwil1, piwil2 and nanos2 and Bcl-2 family genes, bcl-xLb and baxa were significantly reduced during the heat treatment compared to CT, more so in the HT2, while the levels of nanos3 and gfra1 transcripts were only significantly reduced in HT2, implying a significant loss of spermatogonial stem cell (SSC) and spermatogonia in HT2. The effect of HT2 is further evidenced by the significantly reduced sperm density and fertilisation rate compared to CT and HT1 at the end of the recovery period but complete sterility was not induced by HT2. Overall, the present study showed significant effects of HT2 on germ cell survival with histological changes in testes, reduced milt quality, increased 11-KT, and decreased expression of germline-specific genes, SSC marker genes and Bcl-2 family genes in testes which could therefore be potential target genes for sterilisation by genome editing.

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Quercetin inhibits testicular toxicity induced by the mixture of three commonly used phthalates in rats

et al. 2022

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BACKGROUND: Phthalates (PEs), such as butyl benzyl phthalate, dibutyl phthalate and di(2-ethylhexyl) phthalate, are one of the most widely used plasticizers, and humans are increasingly exposed to them. Phytochemical quercetin (Que) is a typical flavonoid with several biological effects, such as antioxidative and anti-inflammatory. The present study was designed to explore the effect of Que on testicular toxicity caused by the mixture of three commonly used PEs (MPEs), and the underlying mechanism. Forty male Sprague-Dawley rats were randomly and equally divided into five groups (n = 8). Rats in control the group were orally treated with the excipient. Rats in the MPEs group were orally administered with 900 mg kg −1 day −1 MPEs, whereas rats in the MPEs+L-Que, MPEs+M-Que and MPEs+H-Que groups were simultaneously treated with 900 mg kg −1 day −1 MPEs and, respectively, 10, 30 and 90 mg kg −1 day −1 Que for 30 days.RESULTS: Compared with the control group, the testes weight, epididymides weight, serum testosterone, luteinizing hormone, follicle-stimulating hormone and estradiol levels, and anogenital distance in the MPEs group were significantly decreased (P < 0.05). The testicular tissues were injured with atrophy of seminiferous tubules, hyperplasia of Leydig cells and arrest of spermatogenesis in the MPEs group. Testicular steroidogenic proteins (StAR, P450scc, CYP17A1 and 17⊎-HSD, P450arom) were up-regulated, whereas P-element-induced wimpy testis proteins (PIWIL1 and PIWIL2) were down-regulated in the MPEs group (P < 0.05). However, the alterations of these parameters were inhibited in the MPEs+M-Que and MPEs+H-Que groups.CONCLUSION: MPEs disturbed steroid hormone metabolism and caused testicular injuries. Que could inhibit testicular toxicity of MPEs, which might relate to the improved regulation of steroid hormone metabolism.

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Suppression effect of LHRH-A and hCG on Piwi expression in testis of Nile tilapia Oreochromis niloticus

Cited by 21 publications

References 30 publications

Expression pattern of nanos, piwil, dnd, vasa and pum genes during ontogenic development in Nile tilapia Oreochromis niloticus

Expression pattern of nanos, piwil, dnd, vasa and pum genes during ontogenic development in Nile tilapia Oreochromis niloticus

Temperature-induced testicular germ cell loss and recovery in Nile tilapia Oreochromis niloticus

Quercetin inhibits testicular toxicity induced by the mixture of three commonly used phthalates in rats

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