Suppression of Arabidopsis AtPUB30 resulted in increased tolerance to salt stress during germination

Hwang, Jae Hwan; Seo, Dong Hye; Kang, Bin G.; Kwak, June M.; Kim, Woo Taek

doi:10.1007/s00299-014-1706-4

Cited by 32 publications

(30 citation statements)

References 56 publications

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“…It appears that challenging Arabidopsis seedlings with ABA or salt stress under continuous light reduces germination efficiencies or cotyledon greening of Col-0 to a greater extent (Reyes and Chua, 2007; Chen et al, 2008; Hwang et al, 2015) compared to long-day conditions (Figure 7; Hu et al, 2013). To tackle the oe-mTERF10 ABA phenotype, we grew Col-0, mterf mutant lines and oe-mTERF10 lines in continuous light and a temperature of 20°C on MS medium (control) and MS medium supplemented with 1 μM ABA, and the phenotypes were scored after 7 days (Figure 9D).…”

Section: Resultsmentioning

confidence: 99%

Arabidopsis thaliana mTERF10 and mTERF11, but Not mTERF12, Are Involved in the Response to Salt Stress

Leister

Kleine

2017

Front. Plant Sci.

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Plastid gene expression (PGE) is crucial for plant development and acclimation to various environmental stress conditions. Members of the “mitochondrial transcription termination factor” (mTERF) family, which are present in both metazoans and plants, are involved in organellar gene expression. Arabidopsis thaliana contains 35 mTERF proteins, of which mTERF10, mTERF11, and mTERF12 were previously assigned to the “chloroplast-associated” group. Here, we show that all three are localized to chloroplast nucleoids, which are associated with PGE. Knock-down of MTERF10, MTERF11, or MTERF12 has no overt phenotypic effect under normal growth conditions. However, in silico analysis of MTERF10, -11, and -12 expression levels points to a possible involvement of mTERF10 and mTERF11 in responses to abiotic stress. Exposing mutant lines for 7 days to moderate heat (30°C) or light stress (400 μmol photons m−2 s−1) fails to induce a phenotype in mterf mutant lines. However, growth on MS medium supplemented with NaCl reveals that overexpression of MTERF11 results in higher salt tolerance. Conversely, mterf10 mutants are hypersensitive to salt stress, while plants that modestly overexpress MTERF10 are markedly less susceptible. Furthermore, MTERF10 overexpression leads to enhanced germination and growth on MS medium supplemented with ABA. These findings point to an involvement of mTERF10 in salt tolerance, possibly through an ABA-mediated mechanism. Thus, characterization of an increasing number of plant mTERF proteins reveals their roles in the response, tolerance and acclimation to different abiotic stresses.

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Section: Resultsmentioning

confidence: 99%

Arabidopsis thaliana mTERF10 and mTERF11, but Not mTERF12, Are Involved in the Response to Salt Stress

Leister

Kleine

2017

Front. Plant Sci.

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“…MtPUB32 also has high sequence similarity with AtPUB13 , which encodes a U-box-ARM protein that links the flowering time and SA-dependent defense signaling pathways in Arabidopsis [12] (S1 Fig and S1 Table). U-box-ARM protein AtPUB30 acts in salt stress tolerance as a negative factor independent of ABA during seed germination [13], and it is homologous to MtPUB38 (S1 Fig). In rice, the U-box-ARM E3 ligase SPL11 negatively regulates plant cell death and defense[14].…”

Section: Resultsmentioning

confidence: 99%

“…AtPUB13 acts as a node that connects flowering time regulation and salicylic acid (SA)-dependent defense signaling in Arabidopsis [12]. AtPUB30 acts in salt stress tolerance as a negative factor whose activity during germination is ABA independent [13]. The roles of PUBs in response to abiotic stresses have also been shown in other plants.…”

Section: Introductionmentioning

confidence: 99%

The U-box family genes in Medicago truncatula: Key elements in response to salt, cold, and drought stresses

Song

Yang

et al. 2017

PLoS ONE

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The ubiquitination pathway regulates growth, development, and stress responses in plants, and the U-box protein family of ubiquitin ligases has important roles in this pathway. Here, 64 putative U-box proteins were identified in the Medicago truncatula genome. In addition to the conserved U-box motif, other functional domains, such as the ARM, kinase, KAP, and WD40 domains, were also detected. Phylogenetic analysis of the M. truncatula U-box proteins grouped them into six subfamilies, and chromosomal mapping and synteny analyses indicated that tandem and segmental duplications may have contributed to the expansion and evolution of the U-box gene family in this species. Using RNA-seq data from M. truncatula seedlings subjected to three different abiotic stresses, we identified 33 stress-inducible plant U-box genes (MtPUBs). Specifically, 25 salinity-, 15 drought-, and 16 cold-regulated MtPUBs were detected. Among them, MtPUB10, MtPUB17, MtPUB18, MtPUB35, MtPUB42, and MtPUB44 responded to all three stress conditions. Expression profiling by qRT-PCR was consistent with the RNA-seq data, and stress-related elements were identified in the promoter regions. The present findings strongly indicate that U-box proteins play critical roles in abiotic stress response in M. truncatula.

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“…It is reported that the pub30‐1 mutant shows salt‐tolerant phenotype only in seed germination stage, whereas there is no significant difference between the pub30‐1 mutant and the wild type in postgerminative growth in response to salt stress (Hwang et al, ). However, in our in vitro (Figure ) and in vivo (Figure ) degradation assay of BKI protein, 10‐day‐old seedlings of WT, pub30‐1 , and MYC‐BKI1 transgenic plants in both genotypes were used because it was very difficult to harvest enough plant sample for protein degradation assay at the seed germination stage.…”

Section: Discussionmentioning

confidence: 99%

“…The negative effect of PUB30 on salt stress tolerance seems to be limited to the seed germination stage (Hwang et al, ). However, BKI1 positively regulates salt tolerance response not only in the seed germination stage but also in seedling growth stage.…”

Section: Discussionmentioning

confidence: 99%

The Arabidopsis U‐box E3 ubiquitin ligase PUB30 negatively regulates salt tolerance by facilitating BRI1 kinase inhibitor 1 (BKI1) degradation

Zhang

Zhao

Long

et al. 2017

Plant Cell & Environment

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The Arabidopsis U-box E3 ubiquitin ligases play an important role in the ubiquitin/26S proteasome-mediated protein degradation pathway. Recently, PUB30 has been reported to participate in the salt stress response during seed germination stage in abscisic acid (ABA)-independent manner, but the molecular mechanism remains to be elucidated. Here, we displayed that the pub30 mutant was more tolerant to salt stress during seed germination, whereas the mutant of its closest homologue PUB31 showed mild sensitivity to salt stress. PUB30 exhibited E3 ubiquitin ligase activity in vitro. PUB30 specifically interacted with BRI1 kinase inhibitor 1 (BKI1), a regulator playing dual roles in brassinosteroids signaling, in vitro and in vivo. We found that BKI1 protein was ubiquitinated and degraded by the 26S proteasome. The degradation of BKI1 was slowed down in the pub30-1 mutant compared with that in the wild type. The bki1 mutant was sensitive to salt, whereas the transgenic plants overexpressing BKI1 showed salt tolerant phenotype. All these results indicate that PUB30 negatively regulates salt tolerance probably through regulating the degradation of BKI1 and brassinosteroids signaling in Arabidopsis.

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Suppression of Arabidopsis AtPUB30 resulted in increased tolerance to salt stress during germination

Cited by 32 publications

References 56 publications

Arabidopsis thaliana mTERF10 and mTERF11, but Not mTERF12, Are Involved in the Response to Salt Stress

Arabidopsis thaliana mTERF10 and mTERF11, but Not mTERF12, Are Involved in the Response to Salt Stress

The U-box family genes in Medicago truncatula: Key elements in response to salt, cold, and drought stresses

The Arabidopsis U‐box E3 ubiquitin ligase PUB30 negatively regulates salt tolerance by facilitating BRI1 kinase inhibitor 1 (BKI1) degradation

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