Suppression of Dextran Sulfate Sodium-Induced Colitis in Mice by Radon Inhalation

Abstract

The enhanced release of reactive oxygen species from activated neutrophils plays important role in the pathogenesis of inflammatory bowel disease. We previously reported that radon inhalation activates antioxidative functions in various organs of mice. In this study, we examined the protective effects of radon inhalation on dextran sulfate sodium- (DSS) induced colitis in mice which were subjected to DSS for 7 days. Mice were continuously treated with air only (sham) or radon at a concentration of 2000 Bq/m3 from a day before DSS administration to the end of colitis induction. In the results, radon inhalation suppressed the elevation of the disease activity index score and histological damage score induced by DSS. Based on the changes in tumor necrosis factor-alpha in plasma and myeloperoxidase activity in the colon, it was shown that radon inhalation suppressed DSS-induced colonic inflammation. Moreover, radon inhalation suppressed lipid peroxidation of the colon induced by DSS. The antioxidant level (superoxide dismutase and total glutathione) in the colon after DSS administration was significantly higher in mice treated with radon than with the sham. These results suggested that radon inhalation suppressed DSS-induced colitis through the enhancement of antioxidative functions in the colon.

Induction of Indoleamine 2,3-dioxygenase by Pre-treatment with Poly(I:C) May Enhance the Efficacy of MSC Treatment in DSS-induced Colitis

“…injection on days 1 and 3 after the administration of DSS. The severity of disease was assessed using the disease activity index (DAI), which evaluates stool consistency and the presence of fecal blood, as previously described (9). …”

RasGRP1 opposes proliferative EGFR–SOS1–Ras signals and restricts intestinal epithelial cell growth

“…At day 5, mice were euthanized and the small and large intestines were processed as described in the immunohistochemistry section. DSS damage scores were determined by the sum of three parameters as previously described 66 : surface epithelial loss, crypt destruction, and inflammatory cell infiltration into the mucosa. A score of ‘0’ represents no change, ‘1’ localized and mild change, ‘2’ localized and moderate change, ‘3’ extensive and moderate change, ‘4’ extensive and severe change.…”

“…( c ) Analysis of mucosal damage following 5 days of DSS treatment. Scores ranging from 0 to 12 were obtained by combining three parameters, as published 66 . Data are depicted as bar graphs with mean score ± s.e.m.…”

FoxP3+ T regulatory cells and immunomodulation after Schistosoma mansoni egg antigen immunization in experimental model of inflammatory bowel disease

“…One hundred and eighty parasite free, Swiss albino male mice, 6-8 weeks old and weighing 25-30 g each were used for this study. According to Nishiyama et al [13], all mice were allowed to adapt for 4 days before the experiments. Mice were kept under conventional conditions, housed in mice cages (15 mice in each), with wire roof, wood chip bed ding at room temperature and a normal light-dark cycle (12:12 h.).…”