Suppression of DNA/RNA and protein oxidation by dietary supplement which contains plant extracts and vitamins: a randomized, double-blind, placebo-controlled trial

Abstract

BackgroundExcessive oxidative stress may impair bio-molecules and cellular function. Multi antioxidant supplementation is thought to be more effective than a single antioxidant probably through the synergistic or complementary action of natural substances that could enhance the prospective effect.MethodsIn order to estimate the effect of a plant extract based supplement in apparently healthy volunteers’ oxidative stress markers, a double-blind and placebo controlled intervention was performed. 62 apparently healthy volunteers, overweight with medium adherence to the Mediterranean diet, were recruited and randomly allocated into two intervention groups (supplement or placebo) for 8 weeks. Basic biochemical markers, oxidized LDL (oxLDL), resistance of serum in oxidation, protein carbonyls in serum and 8-isoprostane and DNA/RNA damage in urine were measured.ResultsNo differentiation was observed in basic biochemical markers, in oxLDL levels as well as in serum resistance against oxidation, during intervention in the examined groups. A significant resistance regarding urine isoprostanes levels in the supplement group compared to the placebo one, was observed. Reduction on DNA/RNA damage and on protein carbonyls levels (almost 30% and 20% respectively, at 8 weeks) was detected in volunteers who consumed the supplement compared to the control group.ConclusionConsumption of plant extract based supplement seems to reduce DNA/RNA and protein oxidation and in less extent lipids peroxidation.Trial registrationClinicalTrials.gov Identifier for this study is: NCT02837107.

Consumption of plant extract supplement reduces platelet activating factor-induced platelet aggregation and increases platelet activating factor catabolism: a randomised, double-blind and placebo-controlled trial

“…Biochemical markers and lifestyle characteristics of volunteers have been previously reported ( 20 ) . Briefly, baseline anthropometric and biochemical markers of the study population did not differ between the two groups.…”

“…Briefly, baseline anthropometric and biochemical markers of the study population did not differ between the two groups. No difference was observed at the levels of glucose, total cholesterol, HDL-cholesterol, LDL-cholesterol, TAG and uric acid between groups at 4 weeks or at 8 weeks of the intervention (online Supplementary Table S1) ( 20 ) . Haematological parameters of volunteers at baseline and during the intervention are presented in Table 1.…”

“…This involves a double-blind, block randomised, parallel-arm, placebo-controlled study, and its duration was 8 weeks. A detailed description of the clinical study has already been reported ( 20 ) and a brief description is presented in the online Supplementary material. A total of fifty-eight healthy volunteers completed the study.…”

“…Haematological parameters such as total leucocyte count, erythrocyte count, Hb level, haematocrit, erythrocyte indices such as mean corpuscular volume, mean corpuscular Hb, mean corpuscular Hb concentration, erythrocyte distribution width, platelet count and mean platelet volume were determined by a Mindray Hematology Analyzer (BC 3000 plus). Enzymatic methods were used to define glucose, TAG, uric acid, total cholesterol, LDL-cholesterol and HDL-cholesterol, as previously described ( 20 ) .…”

“…The aim of the present study is to examine whether a combination of phytochemicals and vitamins with established anti-oxidant effect ( 20 ) may have an impact on PAF actions and metabolism. For this purpose, a double-blind, randomised and placebo-controlled clinical trial was undertaken in apparently healthy adults.…”