Suppression of GhAGP4 gene expression repressed the initiation and elongation of cotton fiber

Li, Yunjing; Liu, Diqiu; Tu, Lili; Zhang, Xianlong; Wang, Li; Zhu, Longfu; Tan, Jiafu; Deng, Fenglin

doi:10.1007/s00299-009-0812-1

Cited by 55 publications

(40 citation statements)

References 42 publications

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“…Cotton DNA and RNA were extracted and transferred to membranes as described previously (Zhu et al, 2005;Li et al, 2010). A fragment of NPTII was used as a probe for Southern blotting for transgene verification.…”

Section: Southern and Northern Blottingmentioning

confidence: 99%

A Genetic and Metabolic Analysis Revealed that Cotton Fiber Cell Development Was Retarded by Flavonoid Naringenin

et al. 2013

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The cotton (Gossypium spp.) fiber is a unique elongated cell that is useful for investigating cell differentiation. Previous studies have demonstrated the importance of factors such as sugar metabolism, the cytoskeleton, and hormones, which are commonly known to be involved in plant cell development, while the secondary metabolites have been less regarded. By mining public data and comparing analyses of fiber from two cotton species (Gossypium hirsutum and Gossypium barbadense), we found that the flavonoid metabolism is active in early fiber cell development. Different flavonoids exhibited distinct effects on fiber development during ovule culture; among them, naringenin (NAR) could significantly retard fiber development. NAR is a substrate of flavanone 3-hydroxylase (F3H), and silencing the F3H gene significantly increased the NAR content of fiber cells. Fiber development was suppressed following F3H silencing, but the overexpression of F3H caused no obvious effects. Significant retardation of fiber growth was observed after the introduction of the F3H-RNA interference segment into the high-flavonoid brown fiber G. hirsutum T586 line by cross. A greater accumulation of NAR as well as much shorter fibers were also observed in the BC1 generation plants. These results suggest that NAR is negatively associated with fiber development and that the metabolism mediated by F3H is important in fiber development, thus highlighting that flavonoid metabolism represents a novel pathway with the potential for cotton fiber improvement.

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Section: Southern and Northern Blottingmentioning

confidence: 99%

A Genetic and Metabolic Analysis Revealed that Cotton Fiber Cell Development Was Retarded by Flavonoid Naringenin

et al. 2013

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“…They were sterilized and then placed in liquid BT medium supplemented with 50 mM H 2 O 2 or 2 mM of the NADPH oxidase inhibitor DPI (Sigma; Li et al, 2010). The samples were collected for RNA extraction after 48 h of culture.…”

Section: Cotton Ovule Culture and Treatment With H 2 O 2 Or Nadph Oximentioning

confidence: 99%

“…The methods of genomic DNA isolation and Southern blotting were according to Li et al (2010). The probe for PDF1 gene detection was the small fragment digested from the cDNA sequence inserted in pSPORT with HindIII (Tu et al, 2007).…”

Section: Southern and Northern Blotting And Qrt-pcrmentioning

confidence: 99%

“…The overexpression vector, RNAi vectors, PGbPDF1::GUS, DBCD, DCD, DD, and PGbPDF1-1::GFP were introduced into cotton (YZ1) plants by A. tumefaciens-mediated (EHA105 and LBA4404) transformation as described by Jin et al (2006) and Li et al (2010). After regeneration, transformed cotton lines were checked by PCR (V-RNAi-F/R for RNAi constructs and V-gus-F/R for GUS-fused vectors) and Southern blotting.…”

Section: Plasmid Construction and Plant Transformationmentioning

confidence: 99%

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GbPDF1 Is Involved in Cotton Fiber Initiation via the Core cis-Element HDZIP2ATATHB2

et al. 2011

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Cotton (Gossypium spp.) fiber cells are seed trichomes derived from the epidermal layer of the cotton seed coat. The molecular components responsible for regulating fiber cell differentiation have not been fully elucidated. A cotton PROTODERMAL FACTOR1 gene (GbPDF1) was found to be expressed preferentially during fiber initiation and early elongation, with highest accumulation in fiber cells 5 d post anthesis. PDF1 silencing caused retardation of fiber initiation and produced shorter fibers and lower lint percentage compared with the wild type, indicating that the gene is required for cotton fiber development. Further analysis showed that a higher accumulation of hydrogen peroxide occurred in the RNA interference transgenic cotton lines. Meanwhile, the expression of several genes related to ethylene and pectin synthesis or sugar transport during cotton fiber growth was found to be significantly reduced in the PDF1-suppressed cotton. Three proteins interacting with GbPDF1 in yeast and in planta might involve cellular signaling or metabolism. GbPDF1 promoter::GUS constructs in transgenic cotton were predominantly expressed in the epidermis of ovules and developing fibers. Progressive deletions of the GbPDF1 promoter showed that a 236-bp promoter fragment was sufficient for basal GbPDF1 transcription in cotton. Mutation of putative regulatory sequences showed that HDZIP2ATATHB2, an element within the fragment, was essential for PGbPDF1-1 expression. The binding activity between this cis-element and nuclear extracts from fiber-bearing cotton ovules at 5 d post anthesis was specific. We conclude that GbPDF1 plays a critical role together with interaction partners in hydrogen peroxide homeostasis and steady biosynthesis of ethylene and pectin during fiber development via the core cis-element HDZIP2ATATHB2.

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“…Furthermore, GhMYB25 and GhMYB25-like regulate early fiber development (Machado et al, 2009;Walford et al, 2011). A study reported that GhAGP4 plays a role in fiber initiation and elongation of cotton, but its molecular mechanism in regulating fiber development still remains unclear (Li et al, 2010b). In our previous study, several fiber-preferential genes encoding FLAs were identified in cotton (Huang et al, 2008).…”

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confidence: 99%

A Fasciclin-Like Arabinogalactan Protein, GhFLA1, Is Involved in Fiber Initiation and Elongation of Cotton

et al. 2013

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Arabinogalactan proteins (AGPs) are involved in many aspects of plant development. In this study, biochemical and genetic approaches demonstrated that AGPs are abundant in developing fibers and may be involved in fiber initiation and elongation. To further investigate the role of AGPs during fiber development, a fasciclin-like arabinogalactan protein gene (GhFLA1) was identified in cotton (Gossypium hirsutum). Overexpression of GhFLA1 in cotton promoted fiber elongation, leading to an increase in fiber length. In contrast, suppression of GhFLA1 expression in cotton slowed down fiber initiation and elongation. As a result, the mature fibers of the transgenic plants were significantly shorter than those of the wild type. In addition, expression levels of GhFLAs and the genes related to primary cell wall biosynthesis were remarkably enhanced in the GhFLA1 overexpression transgenic fibers, whereas the transcripts of these genes were dramatically reduced in the fibers of GhFLA1 RNA interference plants. An immunostaining assay indicated that both AGP composition and primary cell wall composition were changed in the transgenic fibers. The levels of glucose, arabinose, and galactose were also altered in the primary cell wall of the transgenic fibers compared with those of the wild type. Together, our results suggested that GhFLA1 may function in fiber initiation and elongation by affecting AGP composition and the integrity of the primary cell wall matrix.

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Suppression of GhAGP4 gene expression repressed the initiation and elongation of cotton fiber

Cited by 55 publications

References 42 publications

A Genetic and Metabolic Analysis Revealed that Cotton Fiber Cell Development Was Retarded by Flavonoid Naringenin

A Genetic and Metabolic Analysis Revealed that Cotton Fiber Cell Development Was Retarded by Flavonoid Naringenin

GbPDF1 Is Involved in Cotton Fiber Initiation via the Core cis-Element HDZIP2ATATHB2

A Fasciclin-Like Arabinogalactan Protein, GhFLA1, Is Involved in Fiber Initiation and Elongation of Cotton

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