Suppression of Helicobacter pylori-induced gastritis by green tea extract in Mongolian gerbils

Matsubara, Shigeo; Shibata, Hideyuki; Ishikawa, Fumihiko; Yokokura, Teruo; Takahashi, Mami; Sügimura, Takashi; Wakabayashi, Keiji

doi:10.1016/j.bbrc.2003.09.066

Cited by 133 publications

(91 citation statements)

References 28 publications

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“…H. pylori produces a high level of urease, and its urease is located both in the cytoplasm and on the surface layer, which acts as one of the major surface layer proteins. Thus, the reduction in H. pylori urease activity has been used as an indicator for assaying the anti-H. pylori activity of antimicrobial agents (Matsubara et al, 2003;Adeniyi and Anyiam, 2004). In the present study, the presence of DAT and PA effectively reduced the urease activity of susceptible and resistant H. pylori.…”

Section: Discussionmentioning

confidence: 53%

In vitro anti‐Helicobacter pylori activity of diallyl sulphides and protocatechuic acid

Liu

Hsu

Yin

2007

Phytotherapy Research

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The in vitro inhibitory effects of diallyl disulphide (DADS), diallyl trisulphide (DAT), roselle calyx extract and protocatechuic acid (PA) on the growth of Helicobacter pylori (15 susceptible, 11 clarithromycin-resistant and 9 metronidazole-resistant strains) were studied. The inhibition zone was determined after each agent had been heated at 25, 60, 100°C for 60 min. The minimal inhibitory concentration (MIC) of each agent was determined by the tube dilution assay. The results showed that heat treatment did not affect the anti-H. pylori activity of DADS, DAT, roselle calyx extract and PA, and the MIC values of these agents against test H. pylori strains were in the range 8 -64 mg/L. The time-kill study assay for DAT and PA at 1× × × × × MIC was monitored in Muller Hinton broth supplemented with 10% horse blood or mice stomach homogenate. Both DAT and PA inhibited the growth of all test H. pylori in broth and mice stomach homogenate ( p < < < < < 0.05); however, the inhibitory effects of these two agents were less in mice stomach homogenate than in broth (p < < < < < 0.05). DAT at 4, 6, 8, 10, 12, 14 mg/L and PA at 8, 16, 24, 32, 40, 48 mg/L were used for urease activity assay. These two agents significantly reduced urease activity of test H. pylori strains (p < < < < < 0.05), in which DAT and PA at 1× × × × × MIC reduced the urease activity of H. pylori to 70% and 40%, respectively. These agents, based on their lower MIC values and heat tolerance, might be useful in the prevention or therapy of H. pylori.

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Section: Discussionmentioning

confidence: 53%

In vitro anti‐Helicobacter pylori activity of diallyl sulphides and protocatechuic acid

Liu

Hsu

Yin

2007

Phytotherapy Research

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“…The occurrence of antibiotic-resistant H. pylori has been reported, and it is occasionally associated with adverse effects. 5 Therefore, it is still desirable to develop alternative approaches for cancer prevention and a number of studies have demonstrated protective effects of plant extracts against H. pylori infection, such as green tea catechins 6 and a garlic extract. 7 In a previous study, we also found fruit-juice concentrate of Japanese apricot (ume) (CJA) to decrease the number of H. pylori and suppress chronic active gastritis in gerbils, 8 although the mechanism was unclear.…”

mentioning

confidence: 99%

4‐Vinyl‐2,6‐dimethoxyphenol (canolol) suppresses oxidative stress and gastric carcinogenesis in Helicobacter pylori‐infected carcinogen‐treated Mongolian gerbils

Cao

Tsukamoto

Seki

et al. 2008

Intl Journal of Cancer

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Oxidative stress is linked to gastric carcinogenesis because of its ability to damage DNA. Here we examined antioxidative and antiinflammatory effects of 4-vinyl-2,6-dimethoxyphenol (canolol), a recently identified potent antioxidative compound obtained from crude canola oil, on Helicobacter (H.) pylori-induced gastritis and gastric carcinogenesis using a Mongolian gerbil model. The animals were allocated to H. pylori-infection alone (12 weeks) or H. pylori 1 N-methyl-N-nitrosourea (MNU) administration (52 weeks). After oral inoculation of H. pylori, they were fed for 10 and 44 weeks with or without 0.1% canolol. H. pylori-induced gastritis, 5 0 -bromo-2 0 -deoxyuridine (BrdU) labeling and scores for cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) immunohistochemistry were attenuated in the canololtreated groups. Expression of interleukin-1b (IL-1b), tumor necrosis factor-a (TNF-a), COX-2 and iNOS mRNA in the gastric mucosa, and serum 8-hydroxy-2 0 -deoxyguanosine (8-OHdG), anti-H. pylori IgG and gastrin levels were also significantly lower in canolol-treated groups. Furthermore, the incidence of gastric adenocarcinomas was markedly reduced in the H. pylori 1 MNU 1 canolol-treated group [15.0% (6/40)] compared to the control group [39.4% (13/33)] (p < 0.05). These data indicate canolol to be effective for suppressing inflammation, gastric epithelial cell proliferation and gastric carcinogenesis in H. pylori-infected Mongolian gerbils. Interestingly, the viable H. pylori count was not changed by the canolol containing diet. Thus, the data point to the level of inflammation because of H. pylori rather than the existence of the bacteria as the determining factor. Importantly, canolol appears to suppress induction of mRNAs for inflammatory cytokines. ' 2007 Wiley-Liss, Inc.

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“…The inhibitory activities of these extracts were compared with respective positive controls; disodium cromoglycate for the hyaluronidase inhibition 12) , EDTA for the collagenase inhibition 13) , kojic acid for the tyrosinase inhibition 14) , and acetohydroxamic acid and epigallocatechin 3-O-gallate (EGCG) for the urease inhibition 11,15) .…”

Section: Resultsmentioning

confidence: 99%

“…Indeed, other urease inhibitors, including acetohydroxamic acid and EGCG, have been reported to suppress H. pyloriinduced gastritis in Mongolian gerbils 11,15) .…”

Section: The Tyrosinase Activity Was Inhibited About 50% By Thementioning

confidence: 99%

Potential Use of Bischofia javanica as an Active Ingredient of Functional Foods and Cosmeceutical Products Possessing Hyaluronidase, Collagenase, Tyrosinase and Urease Inhibitory Effects

Barla

Horinishi

Harada

et al. 2010

JJCAM

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4 Osaka Women's Junior College [ABSTRACT] Bischofia javanica leaf extract exhibited inhibitory activities against hyaluronidase, collagenase, tyrosinase and urease, suggesting that it is useful as an active ingredient for functional foods and cosmeceutical products. In particular, its 50% inhibitory concentration for hyaluronidase was comparable to that of disodium cromoglycate, which is a well-known hyaluronidase inhibitor used as an anti-inflammation and anti-allergy agent. In addition, the extract also inhibited urease with the almost the same potential as acetohydroxamic acid, which is reported to suppress Helicobacter pyloriinduced gastritis by inhibiting urease.

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Suppression of Helicobacter pylori-induced gastritis by green tea extract in Mongolian gerbils

Cited by 133 publications

References 28 publications

In vitro anti‐Helicobacter pylori activity of diallyl sulphides and protocatechuic acid

In vitro anti‐Helicobacter pylori activity of diallyl sulphides and protocatechuic acid

4‐Vinyl‐2,6‐dimethoxyphenol (canolol) suppresses oxidative stress and gastric carcinogenesis in Helicobacter pylori‐infected carcinogen‐treated Mongolian gerbils

Potential Use of Bischofia javanica as an Active Ingredient of Functional Foods and Cosmeceutical Products Possessing Hyaluronidase, Collagenase, Tyrosinase and Urease Inhibitory Effects

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