Suppression of inflammatory cytokines expression with bitter melon (<i>Momordica charantia</i>) in TNBS-instigated ulcerative colitis

Semiz, Asli; Acar, Ozden Ozgun; Çetin, Hülya; Semiz, Gürkan; Sen, Alaattin

doi:10.2478/jtim-2020-0027

Cited by 17 publications

(11 citation statements)

References 91 publications

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“…And, the predictive efficacy of resistin was significantly better than that of TNF-α. TNF-α and resistin are pro-inflammatory cytokines ( 69 , 70 ). Chronic systemic inflammation has been postulated as a mechanism in the loss of skeletal muscle in COPD.…”

Section: Discussionmentioning

confidence: 99%

Resistin as a Systemic Inflammation-Related Biomarker for Sarcopenia in Patients With Chronic Obstructive Pulmonary Disease

Gao

Deng

et al. 2022

Front. Nutr.

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BackgroundSarcopenia is common in patients with chronic obstructive pulmonary disease (COPD) and is mainly caused by systemic inflammation. Resistin acts as a proinflammatory cytokine and is involved in the activation of multiple inflammatory signaling pathways. The aim of this study was to determine the relationship between resistin levels and systemic inflammation and to assess the clinical value of circulating resistin for sarcopenia in patients with COPD.MethodsIn this prospective observational study, we enrolled 235 patients with COPD who were divided into development and validation sets. The definition of sarcopenia followed the guidelines from the Asian Working Group for Sarcopenia. Serum concentrations of resistin and TNF-α were measured using an enzyme-linked immunosorbent assay (ELISA).ResultsIn this study, higher serum resistin levels were significantly associated with lower skeletal muscle mass and muscular strength. The serum resistin levels in patients with sarcopenia were significantly higher than those in patients without sarcopenia. The serum resistin level had positive correlations with the serum TNF-α level (r = 0.250, p = 0.007). The predictive efficacy of the serum resistin level (AUC: 0.828) for sarcopenia was superior to that of the serum TNF-α level (AUC: 0.621). The cutoff point (7.138 ng/ml) for the serum resistin level was validated in the validation set (AUC: 0.818).ConclusionsSerum resistin levels were associated with systemic inflammation and can be used accurately and easily to predict sarcopenia in patients with COPD.

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Section: Discussionmentioning

confidence: 99%

Resistin as a Systemic Inflammation-Related Biomarker for Sarcopenia in Patients With Chronic Obstructive Pulmonary Disease

Gao

Deng

et al. 2022

Front. Nutr.

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“…Compared to the control group, there was still microscopic damage in the MoC and SS-treatment groups; however, these treatment groups were similar and the results correlated with macroscopic scoring. IL-1β proinflammatory cytokine is released from the colonic macrophage early in inflammation after AA administration and exacerbates mucosal inflammation (32,33) and directly proportional to the severity of inflammation (34). Functional foods are beneficial by reducing pro-inflammatory cytokine expression in models of IBD (35).…”

Section: Discussionmentioning

confidence: 99%

“…Functional foods are beneficial by reducing pro-inflammatory cytokine expression in models of IBD (35). MoC reduced IL-1β levels in DSS-induced colitis and TNBS-induced colitis models (10,15,34).…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Effects of Momordica charantia L. Ethanolic Extract on Acetic Acid-Induced Ulcerative Colitis in Rats

Özbeyli¹,

Şen²,

Aykaç³

et al. 2021

ejb

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Objective: This study aims to investigate the effect of Momordica charantia L. (MoC) ethanolic extract on ulcerative colitis (UC) and was explored in vitro and in vivo. Materials and Methods:The rats were divided into control (C), saline-treated colitis (AA), MoC extract-treated colitis (AA+MoC), and sulfasalazine (SS)-treated colitis (AA+SS) groups. Colitis was induced by acetic acid. MoC extract, SS or saline were given to the related groups for 3 days. Interleukine-1β, malondialdehyde, glutathione levels, myeloperoxidase activity, bax/bcl-2 ratio, caspase-9 and caspase-3 levels were measured in colon and macroscopic and histopathologic examinations were done. Total phenolic/flavonoid content and biological activity of MoC were evaluated by in vitro analysis.Results: Compared to the control group, with acetic acid application interleukin-1β levels, myeloperoxidase activity, malondialdehyde levels, bax/bcl-2 ratio, caspase-9 and caspase-3 levels were significantly upregulated, while glutathione levels were significantly decreased in the AA group. In contrast, MoC and SS treatments reduced interleukin-1β, malondialdehyde levels, myeloperoxidase activity, bax/bcl-2 ratio, and caspase-9 and caspase-3 levels. Glutathione levels increased upon MoC or SS treatment. Increased macroscopic and microscopic scoring with AA improved with MoC or SS treatment, but the MoC or SS treated groups had higher score values than the control. Also, in vitro results showed that MoC exhibited 2,2-diphenyl-1picrylhydrazyl and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical scavenging activity as well as significant antilipoxygenase activity. In addition, MoC extract showed a potent anti-inflammatory activity compared to standard indomethacin. Conclusion:Our biochemical, in vitro and histopathologic analysis indicate that MoC is likely to prove beneficial in UC therapy.

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“…Serum lactate dehydrogenase levels were applied to determine whether treatment protocols had exerted any adverse effects on mice. LDH activities were determined colorimetrically with the BioVision LDH activity assay kit as described . Briefly, LDH activity was determined by measuring the increase in absorbency at 450 nm when LDH reduces NAD to NADH, which then interacts with a probe to produce a color.…”

Section: Methodsmentioning

confidence: 99%

“…LDH activities were determined colorimetrically with the BioVision LDH activity assay kit as described. 45 Briefly, LDH activity was determined by measuring the increase in absorbency at 450 nm when LDH reduces NAD to NADH, which then interacts with a probe to produce a color. One unit of enzymatic activity is defined as the amount of enzyme that catalyzed the oxidation and reduction of 1 μmol of NADH per minute at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Synthesis and Comprehensive in Vivo Activity Profiling of Olean-12-en-28-ol, 3β-Pentacosanoate in Experimental Autoimmune Encephalomyelitis: A Natural Remyelinating and Anti-Inflammatory Agent

et al. 2023

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Multiple sclerosis (MS) treatment has received much attention, yet there is still no certain cure. We herein investigate the therapeutic effect of olean-12-en-28-ol, 3β-pentacosanoate (OPCA) on a preclinical model of MS. First, OPCA was synthesized semisynthetically and characterized. Then, the mice with MOG35–55-induced experimental autoimmune/allergic encephalomyelitis (EAE) were given OPCA along with a reference drug (FTY720). Biochemical, cellular, and molecular analyses were performed in serum and brain tissues to measure anti-inflammatory and neuroprotective responses. OPCA treatment protected EAE-induced changes in mouse brains maintaining blood–brain barrier integrity and preventing inflammation. Moreover, the protein and mRNA levels of MS-related genes such as HLD-DR1, CCL5, TNF-α, IL6, and TGFB1 were significantly reduced in OPCA-treated mouse brains. Notably, the expression of genes, including PLP, MBP, and MAG, involved in the development and structure of myelin was significantly elevated in OPCA-treated EAE. Furthermore, therapeutic OPCA effects included a substantial reduction in pro-inflammatory cytokines in the serum of treated EAE animals. Lastly, following OPCA treatment, the promoter regions for most inflammatory regulators were hypermethylated. These data support that OPCA is a valuable and appealing candidate for human MS treatment since OPCA not only normalizes the pro- and anti-inflammatory immunological bias but also stimulates remyelination in EAE.

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Suppression of inflammatory cytokines expression with bitter melon (Momordica charantia) in TNBS-instigated ulcerative colitis

Cited by 17 publications

References 91 publications

Resistin as a Systemic Inflammation-Related Biomarker for Sarcopenia in Patients With Chronic Obstructive Pulmonary Disease

Resistin as a Systemic Inflammation-Related Biomarker for Sarcopenia in Patients With Chronic Obstructive Pulmonary Disease

Therapeutic Effects of Momordica charantia L. Ethanolic Extract on Acetic Acid-Induced Ulcerative Colitis in Rats

Synthesis and Comprehensive in Vivo Activity Profiling of Olean-12-en-28-ol, 3β-Pentacosanoate in Experimental Autoimmune Encephalomyelitis: A Natural Remyelinating and Anti-Inflammatory Agent

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