Suppression of KIF22 Inhibits Cell Proliferation and Xenograft Tumor Growth in Tongue Squamous Cell Carcinoma

Abstract

Background. Oral carcinoma is the sixth most common cancer and is a serious public health problem, and tongue squamous cell carcinoma (TSCC) is the most common type of oral carcinoma. Kinesin family member 22 (KIF22), also called as kinesin-like DNA binding protein (KID), is a microtubule-based motor protein and binds to both microtubules and chromosomes, transporting organelles, protein, and mRNA. This research aimed at investigating the prognostic significance of KIF22 in TSCC. Patients and Methods. This retrospective research collected 82 paired tissues with TSCC. KIF22 protein expression level was detected by immunohistochemical staining. Suppression of KIF22 with shRNA in CAL-27 and SCC-15 cells was to observe cell proliferation in vitro and xenograft tumor growth in vivo. Results. In TSCC tissues, the protein expression level of KIF22 was increased and correlated with tumor stage, clinical stage, and lymphatic metastasis (P=0.013, P=0.034 and P=0.015, respectively). Suppression of KIF22 inhibited cell proliferation and xenograft tumor growth. Conclusion. KIF22 might play an important role in the progression of TSCC and could serve as a therapeutic target for TSCC.

GBP2 facilitates the progression of glioma via regulation of KIF22/EGFR signaling

“…Several studies have identified KIF22 as an oncogene [ 17 , 20 – 23 ]. Here, we explored the function of KIF22 in glioma cells.…”

“…In prostate cancer patients, high KIF22 expression is correlated with tumor development and poor prognosis [ 20 ]. Studies of KIF22 functions in breast cancer, gastric cancer, and tongue SCC have also been reported [ 21 – 23 ].…”

Blue light aminolevulinic acid photodynamic therapy downregulates cell division and proliferation pathways in cutaneous squamous cell carcinoma

“…MAL PDT downregulated genes associated with proliferation, such as cyclins and the kinesin family member (KIF) proteins, and were similarly identified in our testing with a p < 0.05. KIF proteins are involved with microtubule organization during mitosis and have been previously found to be associated with cancer [28][29][30][31]. Knockdown of KIF22 inhibits tongue SCC proliferation and xenograft tumor growth [28].…”

“…KIF proteins are involved with microtubule organization during mitosis and have been previously found to be associated with cancer [28][29][30][31]. Knockdown of KIF22 inhibits tongue SCC proliferation and xenograft tumor growth [28]. In laboratory studies, PDT treatment protocols decreased cSSC tumor proliferation and increased apoptosis and autophagy via regulation of MAPK protein, particularly AKT [20][21][22].…”

Sodium butyrate inhibits oral squamous cell carcinoma proliferation and invasion by regulating the HDAC1/HSPB7 axis

“…HSPB7 promoter primers were detailed as follows: forward, -CATAGGCCAGTGATGAAGCC--GCTCTGCTGACCCTAACTCTT-Subcutaneous tumorigenesis experiment in nude mice . Referring to the previously reported methods [34,35], eighteen SPF-grade male BALB/c nude mice (5 weeks, 15~18 g) were randomly assigned into three groups: Control+sh-NC group, NaB+sh-NC group, and NaB+sh-HSPB7 group, with six mice in each group. SCC-15 cell suspension at a concentration of about 1 × 10 7 cells/ml was injected into the skin of the left axilla of nude mice with a 1 ml syringe to establish a nude mouse subcutaneous transplantation tumor model.…”