Suppression of Lipid Accumulation by Indole-3-Carbinol Is Associated with Increased Expression of the Aryl Hydrocarbon Receptor and CYP1B1 Proteins in Adipocytes and with Decreased Adipocyte-Stimulated Endothelial Tube Formation

Wang, Mei Lin; Lin, Shyh Hsiang; Hou, Yuan Yu; Chen, Yue Hwa

doi:10.3390/ijms17081256

Cited by 19 publications

(12 citation statements)

References 50 publications

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“…This finding appears contradictory to the mechanical stress‐induced CYP1B1 upregulation that was associated with an impaired adipogenic differentiation. However, Wang et al () demonstrated that an indole‐3‐carbinol‐induced decrease in lipid accumulation in 3 T3‐L1 adipocytes correlated with an increased aryl hydrocarbon receptor (AhR) and CYP1B1 expression. CYP1B1 is regulated via AhR activation, which is considered to play a negative role in adipogenesis.…”

Section: Discussionmentioning

confidence: 99%

The effect of mechanical stress on the proliferation, adipogenic differentiation and gene expression of human adipose-derived stem cells

Paul

Denecke

Kim

et al. 2017

J Tissue Eng Regen Med

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To allow for a better implementation of external volume expansion to clinical applications for soft tissue regeneration, it is necessary to comprehensively understand the underlying mechanisms. As human adipose-derived stem cells (hASCs) play a crucial role in soft tissue enlargement, we investigated the impact of cyclic stretch on gene expression, proliferation rate and adipogenic differentiation of these cells. After cyclic stretching, RNA was extracted and subjected to DNA microarray analysis and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Also, the expression of FABP4 mRNA was analysed by RT-qPCR to test whether mechanical stretch affected adipogenic differentiation of hASCs. The proliferation rate was assessed using the alamarBlue assay and Ki-67 staining. A cell cycle analysis was performed with flow cytometry and Western blot. We found that cyclic stretch significantly induced the expression of CYP1B1 mRNA. Furthermore, the adipogenic differentiation of hASCs was impaired, as was the proliferation. This was partly due to a decrease in extracellular signal-regulated kinase (ERK) 1/2 and histone H3 phosphorylation, suggesting a growth arrest in the G 2 /M phase of the cell cycle. Enrichment analyses demonstrated that stretch-regulated genes were over-represented in pathways and biological processes involved in extracellular matrix organization, vascular remodelling and responses to cell stress. Taken together, mechanical stress impaired both proliferation and adipogenic differentiation, but led to a tissue-remodelling phenotype of hASCs. These data suggest that extracellular matrix remodelling and neoangiogenesis may play a more important role in external volume expansion than proliferation and adipogenesis of hASCs.

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Section: Discussionmentioning

confidence: 99%

The effect of mechanical stress on the proliferation, adipogenic differentiation and gene expression of human adipose-derived stem cells

Paul

Denecke

Kim

et al. 2017

J Tissue Eng Regen Med

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“…84 The AhR agonist indole-3-carbinol decreases lipid accumulation and expression of NRF2, hormone-sensitive lipase (HSL) and GAPDH in mature adipocytes. 85 AhR also affects the lipolysis function of mature adipocytes. Lipolysis functions as an important regulator of systemic energy homeostasis by providing the body with free fatty acids during fasting and exercise.…”

Section: Adipose Tissue and Lipid Metabolismmentioning

confidence: 99%

Environmental factors act through aryl hydrocarbon receptor activation and circadian rhythm disruption to regulate energy metabolism

Khazaal¹,

Jaeger²,

Bottum³

et al. 2018

JRLCR

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“…On this point, a novel study in children and adolescents chronically treated with RSP found that iron status was inversely corelated with weight gain, contributing to dysmetabolic iron overload syndrome (DIOS) ( 135 ). This is significant as intracellular iron increases the risk of ROS generation that cannot be counteracted as Bacteroidetes depletion lowered IPA, a key gut antioxidant ( 98 , 136 , 137 ). Restoration of the adequate levels of AhR and PXR ligands via diet, pre, probiotics, or microbial transplant may upregulate Bacteroidetes phylum, attenuating psychotropic drugs-induced weight gain ( 138 ).…”

Section: Psychotropic Drugs As Antimicrobialsmentioning

confidence: 99%

The Other Obesity Epidemic—Of Drugs and Bugs

et al. 2020

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Chronic psychiatric patients with schizophrenia and related disorders are frequently treatment-resistant and may require higher doses of psychotropic drugs to remain stable. Prolonged exposure to these agents increases the risk of weight gain and cardiometabolic disorders, leading to poorer outcomes and higher medical cost. It is well-established that obesity has reached epidemic proportions throughout the world, however it is less known that its rates are two to three times higher in mentally ill patients compared to the general population. Psychotropic drugs have emerged as a major cause of weight gain, pointing to an urgent need for novel interventions to attenuate this unintended consequence. Recently, the gut microbial community has been linked to psychotropic drugs-induced obesity as these agents were found to possess antimicrobial properties and trigger intestinal dysbiosis, depleting Bacteroidetes phylum. Since germ-free animals exposed to psychotropics have not demonstrated weight gain, altered commensal flora composition is believed to be necessary and sufficient to induce dysmetabolism. Conversely, not only do psychotropics disrupt the composition of gut microbiota but the later alter the metabolism of the former. Here we review the role of gut bacterial community in psychotropic drugs metabolism and dysbiosis. We discuss potential biomarkers reflecting the status of Bacteroidetes phylum and take a closer look at nutritional interventions, fecal microbiota transplantation, and transcranial magnetic stimulation, strategies that may lower obesity rates in chronic psychiatric patients.

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Suppression of Lipid Accumulation by Indole-3-Carbinol Is Associated with Increased Expression of the Aryl Hydrocarbon Receptor and CYP1B1 Proteins in Adipocytes and with Decreased Adipocyte-Stimulated Endothelial Tube Formation

Cited by 19 publications

References 50 publications

The effect of mechanical stress on the proliferation, adipogenic differentiation and gene expression of human adipose-derived stem cells

The effect of mechanical stress on the proliferation, adipogenic differentiation and gene expression of human adipose-derived stem cells

Environmental factors act through aryl hydrocarbon receptor activation and circadian rhythm disruption to regulate energy metabolism

The Other Obesity Epidemic—Of Drugs and Bugs

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