Suppression of Lipid Accumulation in 3T3-L1 Adipocytes by α-Tocopheryl Succinate

Majima, Dai; Mitsuhashi, Ryosuke; Yamasaki, Misaki; Kajimoto, Kazuaki; Fukuta, Tatsuya; Kogure, Kentaro

doi:10.1248/bpb.b20-00573

Cited by 3 publications

(3 citation statements)

References 17 publications

(20 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previously, FMN exhibited partial PPAR‐γ agonistic attribute by lowering lipid and promoting insulin sensitivity in differentiated preadipocytes [23] . In, contrast VES suppressed adipogenesis by inhibiting lipid synthesis [24] . In support to this observation, our group also reported Phloretin and Phloridzin individually and in combination showed an enhancement in lipid and triglyceride accumulation by improving their insulin sensitivity in differentiated adipocytes [25] .…”

Section: Resultssupporting

confidence: 67%

“…[23] In, contrast VES suppressed adipogenesis by inhibiting lipid synthesis. [24] In support to this observation, our group also reported Phloretin and Phloridzin individually and in combination showed an enhancement in lipid and triglyceride accumulation by improving their insulin sensitivity in differentiated adipocytes. [25] Further, insulin-sensitizing potency was evaluated by monitoring the rate of glucose uptake using flow cytometry.…”

Section: Vesylated-fmn Promoted Adipogenesismentioning

confidence: 52%

See 1 more Smart Citation

Formononetin‐Vitamin E Conjugate Synergistically Supports Adipogenesis, Attenuates Oxidative Stress, and Restores Insulin Sensitization in Differentiated Preadipocytes

Kumar Dhritlahre,

Goel,

Rana

et al. 2024

ChemistrySelect

View full text Add to dashboard Cite

In this study, a hybrid conjugate of formononetin (FMN) and vitamin E (VESylated‐FMN) was synthesized through succinic acid linker and investigated for its synergistic effect in diabetes. The conjugate was characterized through Ultra Performance Liquid Chromatography – Mass Spectrometry (UPLC‐MS), High‐Resolution Mass Spectrometry (HRMS), Nuclear Magnetic Resonance (NMR), and Differential Scanning Calorimetry (DSC). The ability of VESylated‐FMN to promote adipogenesis and ameliorate insulin resistance brought on by oxidative stress was evaluated in differentiated preadipocytes. Interestingly, VESylated‐FMN promoted intracellular glucose absorption, which in turn increased lipid and triglyceride accumulation and thereby encouraged adipogenesis. VESylated‐FMN attenuated oxidative stress and promoted cell survival by reducing reactive oxygen species (ROS) generation and reversing apoptosis. Additionally, VESylated‐FMN improved insulin sensitization by upregulating the gene and protein expression of insulin‐sensitizing markers. Overall, FMN in conjugation with vitamin E can effectively augment prior insulin sensitivity by reducing oxidative stress for efficient glycemic control in hyperglycemia.

show abstract

Section: Resultssupporting

confidence: 67%

Section: Vesylated-fmn Promoted Adipogenesismentioning

confidence: 52%

Formononetin‐Vitamin E Conjugate Synergistically Supports Adipogenesis, Attenuates Oxidative Stress, and Restores Insulin Sensitization in Differentiated Preadipocytes

Kumar Dhritlahre,

Goel,

Rana

et al. 2024

ChemistrySelect

View full text Add to dashboard Cite

show abstract

“…CLA has been reported to reduce adipocyte size in body fat mass [ 26 ]. It has also been reported that α-tocopherol induces the change of mature adipocytes to beige adipocytes, which are cells with a reduced lipid droplet size [ 27 ]. The differentiated adipocytes have the ability to store TG in lipid droplets [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Facile adipocyte uptake and liver/adipose tissue delivery of conjugated linoleic acid-loaded tocol nanocarriers for a synergistic anti-adipogenesis effect

Hsu,

Liao,

Lin

et al. 2024

J Nanobiotechnol

View full text Add to dashboard Cite

Obesity is a major risk to human health. Adipogenesis is blocked by α-tocopherol and conjugated linoleic acid (CLA). However, their effect at preventing obesity is uncertain. The effectiveness of the bioactive agents is associated with their delivery method. Herein, we designed CLA-loaded tocol nanostructured lipid carriers (NLCs) for enhancing the anti-adipogenic activity of α-tocopherol and CLA. Adipogenesis inhibition by the nanocarriers was examined using an in vitro adipocyte model and an in vivo rat model fed a high fat diet (HFD). The targeting of the tocol NLCs into adipocytes and adipose tissues were also investigated. A synergistic anti-adipogenesis effect was observed for the combination of free α-tocopherol and CLA. Nanoparticles with different amounts of solid lipid were developed with an average size of 121‒151 nm. The NLCs with the smallest size (121 nm) showed greater adipocyte internalization and differentiation prevention than the larger size. The small-sized NLCs promoted CLA delivery into adipocytes by 5.5-fold as compared to free control. The nanocarriers reduced fat accumulation in adipocytes by counteracting the expression of the adipogenic transcription factors peroxisome proliferator activated receptor (PPAR)γ and CCAAT/enhancer-binding protein (C/EBP)α, and lipogenic enzymes acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS). Localized administration of CLA-loaded tocol NLCs significantly reduced body weight, total cholesterol, and liver damage indicators in obese rats. The biodistribution study demonstrated that the nanoparticles mainly accumulated in liver and adipose tissues. The NLCs decreased adipocyte hypertrophy and cytokine overexpression in the groin and epididymis to a greater degree than the combination of free α-tocopherol and CLA. In conclusion, the lipid-based nanocarriers were verified to inhibit adipogenesis in an efficient and safe way.

show abstract

Development of a novel tocopheryl ester for suppression of lipid accumulation without cytotoxicity by optimization of dicarboxylic ester moiety

Yamasaki

Seto²,

Ozono³

et al. 2022

Biochemistry and Biophysics Reports

View full text Add to dashboard Cite

Suppression of Lipid Accumulation in 3T3-L1 Adipocytes by α-Tocopheryl Succinate

Cited by 3 publications

References 17 publications

Formononetin‐Vitamin E Conjugate Synergistically Supports Adipogenesis, Attenuates Oxidative Stress, and Restores Insulin Sensitization in Differentiated Preadipocytes

Formononetin‐Vitamin E Conjugate Synergistically Supports Adipogenesis, Attenuates Oxidative Stress, and Restores Insulin Sensitization in Differentiated Preadipocytes

Facile adipocyte uptake and liver/adipose tissue delivery of conjugated linoleic acid-loaded tocol nanocarriers for a synergistic anti-adipogenesis effect

Development of a novel tocopheryl ester for suppression of lipid accumulation without cytotoxicity by optimization of dicarboxylic ester moiety

Contact Info

Product

Resources

About