Suppression of miRNA-708 by Polycomb Group Promotes Metastases by Calcium-Induced Cell Migration

Ryu, Seongho; McDonnell, Kevin; Choi, Hyejin; Gao, Dingcheng; Hahn, Mary; Joshi, Natasha; Park, Sun Mi; Catena, Raúl; Do, Yoonkyung; Brazin, Jacqueline; Vahdat, Linda T.; Silver, Randi B.; Mittal, Vivek

doi:10.1016/j.ccr.2012.11.019

Cited by 139 publications

(155 citation statements)

References 64 publications

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“…According to our unpublished data of NGS results between MDA-MB-231 and MCF7, miR-218 is higher in MDA-MB-231 while miR-708 is lower in MDA-MB-231 comparing to MCF7. In addition, miR-708 expression was validated in our previous study [25]. In consistence with NGS data and previous study, miR-218 had higher expression levels in the metastatic breast cancer cells (MDA-MB-231) than in the non-metastatic cells (MCF7).…”

Section: Resultssupporting

confidence: 66%

mirRICH, a simple method to enrich the small RNA fraction from over-dried RNA pellets

et al. 2018

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Techniques to isolate the small RNA fraction (<200nt) by column-based methods are commercially available. However, their use is limited because of the relatively high cost. We found that large RNA molecules, including mRNAs and rRNAs, are aggregated together in the presence of salts when RNA pellets are over-dried. Moreover, once RNA pellets are over-dried, large RNA molecules are barely soluble again during the elution process, whereas small RNA molecules (<100nt) can be eluted. We therefore modified the acid guanidinium thiocyanate-phenol-chloroform (AGPC)-based RNA extraction protocol by skipping the 70% ethanol washing step and over-drying the RNA pellet for 1 hour at room temperature. We named this novel small RNA isolation method “mirRICH.” The quality of the small RNA sequences was validated by electrophoresis, next-generation sequencing, and quantitative PCR, and the findings support that our newly developed column-free method can successfully and efficiently isolate small RNAs from over-dried RNA pellets.

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Section: Resultssupporting

confidence: 66%

mirRICH, a simple method to enrich the small RNA fraction from over-dried RNA pellets

et al. 2018

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“…However, this study did not report the expression level of the TMEM88 protein, and it is therefore unclear whether the enhanced invasion of NSCLC cells was due to suppression of TMEM88 transcription or some other mechanism. Moreover, conflicting reports have suggested that miR708 suppresses migration and invasion of tumor cells (33)(34)(35).…”

Section: Discussionmentioning

confidence: 99%

Cytosolic TMEM88 Promotes Invasion and Metastasis in Lung Cancer Cells by Binding DVLS

Zhang

Jiang

et al. 2015

Cancer Research

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Transmembrane protein 88 (TMEM88) is a transmembrane protein that plays a crucial role in regulating human stem cell differentiation and embryonic development. However, its expression and clinicopathologic significance in human neoplasms is unclear. In this study, the expression and subcellular localizations of TMEM88 were assessed in 214 cases of non-small cell lung cancer (NSCLC). Notably, TMEM88 was highly expressed in the cytosol of $60% NSCLC specimens examined. Higher expression of cytosolic TMEM88 in NSCLC correlated significantly with poor differentiation, high TNM stage, lymph node metastasis, and inferior survival. In NSCLC cells displaying membrane-localized TMEM88, we observed an inhibition of canonical Wnt signaling due to interactions of TMEM88 with the Wnt pathway factor Dishevelled (DVLS). In contrast, NSCLC cells with cytosol-localized TMEM88 lacked effects on Wnt signaling. Cytosolic interactions of TMEM88 and DVLS increased the expression of phosphorylated, active forms of p38, GSK3b (Thr390), and Snail, thereby reducing the expression of the tight junction-associated proteins ZO-1 and occludin, effects associated with enhanced invasive and metastatic cell characters. Importantly, attenuating the expression of cytosolic TMEM88 reduced metastatic prowess in xenograft models. Overall, our findings show how mislocalization of TMEM88 to the cytosol in NSCLC cells ablates its Wnt pathway regulatory properties, thereby promoting invasion and metastasis by activating the p38-GSK3b-Snail signaling pathway. Cancer Res; 75(21); 4527-37. Ó2015 AACR.

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“…We also found that some genes previously linked to GABAergic interneuron differentiation, such as Sox11, were expressed by cells in both cluster 2 and 3 ( Figure 4M-P). Cells in the lateral margin of the MGE expressed genes belonging to cluster 4/5, including the cytoskeleton regulator Gap43 [34] and the transmembrane protein gene neuronatin (Nnat) ( Figure 4U-X), both of which have previously been linked to cell migration [34,35]. The expression of several genes unique to cluster 4/ 5 is maintained in migrating interneurons as they propagate towards the cortex.…”

Section: Spatial Pattern Validation Of Medial Ganglionic Eminence Tramentioning

confidence: 99%

Topographical transcriptome mapping of the mouse medial ganglionic eminence by spatially-resolved RNA-seq

Zechel¹,

Zając²,

Lönnerberg³

et al. 2014

Genome Biol

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Background: Cortical interneurons originating from the medial ganglionic eminence, MGE, are among the most diverse cells within the CNS. Different pools of proliferating progenitor cells are thought to exist in the ventricular zone of the MGE, but whether the underlying subventricular and mantle regions of the MGE are spatially patterned has not yet been addressed. Here, we combined laser-capture microdissection and multiplex RNA-sequencing to map the transcriptome of MGE cells at a spatial resolution of 50 μm. Results: Distinct groups of progenitor cells showing different stages of interneuron maturation are identified and topographically mapped based on their genome-wide transcriptional pattern. Although proliferating potential decreased rather abruptly outside the ventricular zone, a ventro-lateral gradient of increasing migratory capacity was identified, revealing heterogeneous cell populations within this neurogenic structure.

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Suppression of miRNA-708 by Polycomb Group Promotes Metastases by Calcium-Induced Cell Migration

Cited by 139 publications

References 64 publications

mirRICH, a simple method to enrich the small RNA fraction from over-dried RNA pellets

mirRICH, a simple method to enrich the small RNA fraction from over-dried RNA pellets

Cytosolic TMEM88 Promotes Invasion and Metastasis in Lung Cancer Cells by Binding DVLS

Topographical transcriptome mapping of the mouse medial ganglionic eminence by spatially-resolved RNA-seq

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