Suppression of Murine Lymphocyte Mitogen Responses by Exopolysaccharide from
            <i>Capnocytophaga ochracea</i>

Bolton, Ronald W.; Dyer, John K.

doi:10.1128/iai.39.1.476-479.1983

Cited by 25 publications

(8 citation statements)

References 14 publications

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“…com/onlineopen#OnlineOpen_Terms production of oral bacteria is associated with persistent infections in the oral cavity (Yamane et al 2009. Capnocytophaga ochracea found in periodontal disease has been shown to produce mannose-rich EPS that can suppress murine lymphocyte mitogen responses and activate human complement response (Bolton & Dyer 1983, 1986, Dyer & Bolton 1985. It was reported that some clinical isolates of Prevotella intermedia and P. nigrescens isolated from chronic periodontitis lesions produce EPSs in a sucrose-independent manner and form biofilms that contribute to their virulence (Fukushima et al 1992, Yamane et al 2005, Yamanaka et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Pathogenicity of exopolysaccharide‐producing Actinomyces oris isolated from an apical abscess lesion

et al. 2012

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Aim To demonstrate a capacity for producing exopolysaccharides (EPSs) and an ability to form biofilm on abiotic materials of Actinomyces oris strain K20. Methodology The productivity of EPSs and the ability to form biofilm of strain K20 were evaluated by measuring viscosity of spent culture media and by scanning electron microscopy (SEM) and the biofilm assay on microtitre plates, respectively. High‐performance liquid chromatography was used to determine the chemical composition of the viscous materials. To examine the role of the viscous materials attributable to the pathogenicity in this organism, the ability of strain K20 to induce abscess formation was compared in mice to that of ATCC 27044. Results The viscosity of the spent culture media of K20 was significantly higher than that of ATCC 27044. Strain K20 showed dense meshwork structures around the cells and formed biofilms on microtitre plates, whereas ATCC 27044 did not. Chemical analysis of the viscous materials revealed that they were mainly composed of neutral sugars with mannose constituting 77.5% of the polysaccharides. Strain K20 induced persistent abscesses in mice lasting at least 5 days at a concentration of 108 cells mL−1, whereas abscesses induced by ATCC 27044 healed and disappeared or decreased in size at day 5. Conclusions Strain K20 produced EPSs, mainly consisting of mannose, and formed biofilms. This phenotype might play an important role for A. oris to express virulence through the progression of apical periodontitis.

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Section: Introductionmentioning

confidence: 99%

Pathogenicity of exopolysaccharide‐producing Actinomyces oris isolated from an apical abscess lesion

et al. 2012

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“…Moreover, these microorganisms have several features which would warrant their being considered periodontal pathogens. They have been shown to produce an immunosuppressive exopolysaccharide (5,6), to adversely affect neutrophil function (58), and to produce a bone-resorbing lipopolysaccharide (31), and they have been implicated to have tissue-invasive capabilities (11). In addition, they have been shown to hydrolyze immunoglobulins A and G (33) and to produce a superoxide dismutase (25).…”

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Lysozyme-mediated aggregation and lysis of the periodontal microorganism Capnocytophaga gingivalis 2010

Iacono¹,

Zove²,

Grossbard³

et al. 1985

Infect Immun

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The ability of lysozyme to aggregate and lyse the gram-negative capnophilic periodontal microorganism Capnocytophaga gingivalis 2010 was monitored optically at 540 nm. Both hen egg white and chromatographically purified human lysozymes had significant but similar aggregation potentials for both logarithmicand stationary-phase bacteria. In general, an increase in enzyme concentration resulted in a graded increase in both the initial and maximum changes in turbidity which occurred during the reaction period. The greatest change in turbidity occurred within the initial minutes of interaction of lysozyme and the cells, and the extent of aggregation paralleled a rapid depletion of lysozyme by the suspensions during the first minute of its incubation with the bacteria. Interestingly, the muramidase inhibitors N-acetyl-D-glucosamine and histamine did not block aggregation, whereas maleylation of lysozyme completely inhibited its aggregating ability. Demaleylation, however, restored aggregation activity comparable to the native enzyme, indicating that maleylated lysozyme retained its integrity and that aggregation was primarily dependent on charge. The addition of up to physiological concentrations of NaHCO3 and NaCl to cell aggregates resulted in varying degrees of deaggregation and lysis. Surprisingly, ultrastructural analysis of lysozyme-treated cells revealed morphological changes with or without the addition of salt. Damage appeared to occur at the blunted polar end of the cells where there was a large spherical outpouching bordered by a damaged cell envelope. Damaged cells uniformly contained dense granular cytoplasmic debris. In effect, the cationic enzyme lysed C. gingivalis 2010, which was not apparent in the spectrophotometric assay. The paradoxical finding that during bacterial aggregation there was lysis may be of significance to the further elucidation of lysozyme's antibacterial role in the gingival sulcus.

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“…Among the components of subgingival plaque, anaerobic gram-negative rods including Captwcytophaga spp., Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans have been implicated as etiological agents in periodontal disease (10,19). Possible virulence factors of Capnocytophaga such as an inhibitor of chemotaxis of polymorphonuclear leukocytes (18), a lymphocyte alteration factor (1), an immunoglobulin protease (3,13), phospholipase A2 (17) and superoxide dismutase (8) have been characterized. However, growth of this microorganism in the periodontal area is necessary for these pathogenic factors to affect the periodontium.…”

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confidence: 99%

Isolation and properties of the Capnocytophaga ochracea bacteriocin

Nakamura

Shibata

Shimura

et al. 1992

Oral Microbiology and Immunology

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Suppression of Murine Lymphocyte Mitogen Responses by Exopolysaccharide from Capnocytophaga ochracea

Cited by 25 publications

References 14 publications

Pathogenicity of exopolysaccharide‐producing Actinomyces oris isolated from an apical abscess lesion

Pathogenicity of exopolysaccharide‐producing Actinomyces oris isolated from an apical abscess lesion

Lysozyme-mediated aggregation and lysis of the periodontal microorganism Capnocytophaga gingivalis 2010

Isolation and properties of the Capnocytophaga ochracea bacteriocin

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