Suppression of norovirus by natural phytochemicals from Aloe vera and Eriobotryae Folium

Ng, Yee Ching; Kim, Hyoun‐Ah; Ryu, Seungbo; Lee, Ariel; Lee, Jeong Su; Song, Mee Hyun

doi:10.1016/j.foodcont.2016.10.051

Cited by 19 publications

(12 citation statements)

References 43 publications

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Section: Methodsmentioning

confidence: 99%

“…The total RNA was extracted from cells using the TRI Reagent (Molecular Research Center, Inc.) and chloroform extraction methods ( 29 ). The cDNA was synthesized with random hexamer primers using RevertAid First strand cDNA synthesis (Fermentas, USA) as previously described ( 29 ). The cellular transcripts were quantified with the following primers: human DHX9 (F: 5′-tccaactggaatccttggac-3′; R: 5′-ttttcccacatccagtagcc-3′), human IL6 (F: 5′-agccctgagaaaggagacatgta-3′; R: 5′-tctgccagtgcctctttgc-3′), human IFN-β (F: 5′-tgacatccctgaggagattaagc-3′; R: 5′-gcgtcctccttctggaactg-3′), murine Dhx9 (F: 5′-cccccacctgttgatccttc-3′; R: 5′-gaccaaggaaccactcccac-3′), murine Il-6 (F: 5′-tccatccagttgccttcttg-3′; R: 5′-ggtctgttgggagtggtatc-3′), murine Ifn-β (F: 5′-aaactcatgaccagtctgca-3′; R: 5′-aggagatcttcagtttcggagc-3′), murine Tnf (F: 5′-cgtcagccgatttgctatct-3′; R: 5′-cggactccgcaaagtctaag-3′), murine Il-1β (F: 5′-caaccaacaagtgatattctccatg-3′; R: 5′-gatccacactctccagctgca-3′), ICP0 (F: 5′-aagcttggatccgagccccgccc-3′; R: 5′-aagcggtgcatgcacgggaaggt-3′), RTA (F: 5′-gcctggaggcgcttagg-3′; R: 5′-aacacattgcgcccaaatg-3′).…”

Section: Methodsmentioning

confidence: 99%

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A DNA-sensing–independent role of a nuclear RNA helicase, DHX9, in stimulation of NF-κB–mediated innate immunity against DNA virus infection

Chung

Kang

et al. 2018

Nucleic Acids Research

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DExD/H-box helicase 9 (DHX9), or RNA helicase A (RHA), is an abundant multifunctional nuclear protein. Although it was previously reported to act as a cytosolic DNA sensor in plasmacytoid dendritic cells (pDCs), the role and molecular mechanisms of action of DHX9 in cells that are not pDCs during DNA virus infection are not clear. Here, a macrophage-specific knockout and a fibroblast-specific knockdown of DHX9 impaired antiviral innate immunity against DNA viruses, leading to increased virus replication. DHX9 enhanced NF-κB–mediated transactivation in the nucleus, which required its ATPase-dependent helicase (ATPase/helicase) domain, but not the cytosolic DNA-sensing domain. In addition, DNA virus infection did not induce cytoplasmic translocation of nuclear DHX9 in macrophages and fibroblasts. Nuclear DHX9 was associated with a multiprotein complex including both NF-κB p65 and RNA polymerase II (RNAPII) in chromatin containing NF-κB–binding sites. DHX9 was essential for the recruitment of RNAPII rather than NF-κB p65, to the corresponding promoters; this function also required its ATPase/helicase activity. Taken together, our results show a critical role of nuclear DHX9 (as a transcription coactivator) in the stimulation of NF-κB–mediated innate immunity against DNA virus infection, independently of DHX9’s DNA-sensing function.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A DNA-sensing–independent role of a nuclear RNA helicase, DHX9, in stimulation of NF-κB–mediated innate immunity against DNA virus infection

Chung

Kang

et al. 2018

Nucleic Acids Research

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“…Furthermore, other natural plant‐derived substances, such as aloe vera and Eriobotryae folium extracts, showed antiviral activity against MNV (Ng et al., ) and could be incorporated into food packaging materials in future studies.…”

Section: Development Of Food‐grade Polymers and Biopolymers With Antimentioning

confidence: 99%

Polymers and Biopolymers with Antiviral Activity: Potential Applications for Improving Food Safety

Randazzo

Fabra

Falcó

et al. 2018

Comp Rev Food Sci Food Safe

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Gastroenteritis and hepatitis, caused by human noroviruses (HuNoVs) and hepatitis A virus (HAV), respectively, are the most common illnesses resulting from the consumption of food contaminated with human enteric viruses. Food-grade polymers can be tailor designed to improve food safety, either as novel food-packaging materials imparting active antimicrobial properties, applied in food contact surfaces to avoid cross-contamination, or as edible coatings to increase fresh produce's shelf life. The incorporation of antimicrobial agents into food-grade polymers can be used to control the food microbiota and even target specific foodborne pathogens to improve microbiological food safety and to enhance food quality. Enteric viruses are responsible for one fifth of acute gastroenteritis cases worldwide and the development of food-grade polymers and biopolymers with antiviral activity for food applications is a topic of increased interest, both for academia and the food industry, even though developments are still limited. This review compiles existing studies in this widely unexplored area and highlights the potential of these developments to improve viral food safety.

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“…MNV-1 is remarkably stable and can endure harsh conditions such as low pH, freezing temperatures, thermal inactivation, and exposure to organic solvent/alcohol-based disinfectants; moreover, it is persistent on surfaces under dry and wet conditions [ 4 , 10 ]. MNV-1 is also easy to culture, and thus has been employed in several norovirus inactivation studies [ 11 , 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Antiviral Effects of Lindera obtusiloba Leaf Extract on Murine Norovirus-1 (MNV-1), a Human Norovirus Surrogate, and Potential Application to Model Foods

et al. 2020

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Noroviruses are the leading cause of acute gastroenteritis and food poisoning worldwide. In this study, we investigated the anti-noroviral activity of Lindera obtusiloba leaf extract (LOLE) using murine norovirus (MNV-1), a surrogate of human norovirus. Preincubation of MNV-1 with LOLE at 4, 8, or 12 mg/mL for 1 h at 25 °C significantly reduced viral infectivity, by 51.8%, 64.1%, and 71.2%, respectively. Among LOLE single compounds, β-pinene (49.7%), α-phellandrene (26.2%), and (+)-limonene (17.0%) demonstrated significant inhibitory effects on viral infectivity after pretreatment with MNV-1, suggesting that the anti-noroviral effects of LOLE may be due to the synergetic activity of several compounds, with β-pinene as a key molecule. The inhibitory effect of LOLE was tested on the edible surfaces of lettuce, cabbage, and oysters, as well as on stainless steel. After one hour of incubation at 25°C, LOLE (12 mg/mL) pretreatment significantly reduced MNV-1 plaque formation on lettuce (76.4%), cabbage (60.0%), oyster (38.2%), and stainless-steel (62.8%). These results suggest that LOLE effectively inhibits norovirus on food and metal surfaces. In summary, LOLE, including β-pinene, may inactivate norovirus and could be used as a natural agent promoting food safety and hygiene.

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Suppression of norovirus by natural phytochemicals from Aloe vera and Eriobotryae Folium

Cited by 19 publications

References 43 publications

A DNA-sensing–independent role of a nuclear RNA helicase, DHX9, in stimulation of NF-κB–mediated innate immunity against DNA virus infection

A DNA-sensing–independent role of a nuclear RNA helicase, DHX9, in stimulation of NF-κB–mediated innate immunity against DNA virus infection

Polymers and Biopolymers with Antiviral Activity: Potential Applications for Improving Food Safety

Antiviral Effects of Lindera obtusiloba Leaf Extract on Murine Norovirus-1 (MNV-1), a Human Norovirus Surrogate, and Potential Application to Model Foods

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