Suppression of protein inactivation during freezing by minimizing pH changes using ionic cryoprotectants

Krausková, Ľubica; Procházková, Jitka; Klašková, Martina; Filipová, Lenka; Chaloupková, Radka; Malý, Stanislav; Damborský, Jiřı́; Heger, Dominik

doi:10.1016/j.ijpharm.2016.05.031

Cited by 52 publications

(32 citation statements)

References 70 publications

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“…Unlike synthetic chemicals, biomaterials such as alginates, polyvinyl alcohol, and chitosan can be used to impede ice crystal growth, along with traditional small molecules 4 . The direct inhibition of ice crystal formation and application of antioxidants and other compounds have been used to attempt to reduce cell death from processes such as apoptosis during the freezing and thawing cycle 20, 21, 22, 23. Common CPAs are briefly addressed in the following subsections and Table 2.…”

Section: Cryopreservationmentioning

confidence: 99%

Cryopreservation and its clinical applications

Jang

Park

Yang

et al. 2017

Integrative Medicine Research

366

234

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Cryopreservation is a process that preserves organelles, cells, tissues, or any other biological constructs by cooling the samples to very low temperatures. The responses of living cells to ice formation are of theoretical interest and practical relevance. Stem cells and other viable tissues, which have great potential for use in basic research as well as for many medical applications, cannot be stored with simple cooling or freezing for a long time because ice crystal formation, osmotic shock, and membrane damage during freezing and thawing will cause cell death. The successful cryopreservation of cells and tissues has been gradually increasing in recent years, with the use of cryoprotective agents and temperature control equipment. Continuous understanding of the physical and chemical properties that occur in the freezing and thawing cycle will be necessary for the successful cryopreservation of cells or tissues and their clinical applications. In this review, we briefly address representative cryopreservation processes, such as slow freezing and vitrification, and the available cryoprotective agents. In addition, some adverse effects of cryopreservation are mentioned.

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Section: Cryopreservationmentioning

confidence: 99%

Cryopreservation and its clinical applications

Jang

Park

Yang

et al. 2017

Integrative Medicine Research

366

234

View full text Add to dashboard Cite

show abstract

“…In contrast, the dynamics of freezing forces the solutes to segregate and form the veins of freeze-concentrated solutions engulfed by the ice (Blackford, 2007;Cheng et al, 2010;McCarthy et al, 2013;Bogdan et al, 2014;Krausko et al, 2014). The solutes in the freeze-concentrated solution, here probably in the surface layer only, experience not only an increased concentration (Heger et al, 2005;Kania et al, 2014;Krausko et al, 2015a) but also a changed pH (Heger et al, 2006;Krausková et al, 2016;Papadimitriou et al, 2016;Rérolle et al, 2016) and polarity (Heger and Klan, 2007). Recently, it was noticed that, for a frozen solution, the surface brine layer is interconnected with the interior veins system (Walker et al, 2013).…”

Section: Ffs At a High Temperature: Brine Fingers Formationmentioning

confidence: 99%

“…Such a model is generally unsatisfactory in describing the real situation, and thus more realistic parameters for modelling are needed. Some of us previously showed that the concentration increase of nonpolar (Heger et al, 2011;Kania et al, 2014;Krausko et al, 2015a, b) and polar compounds (Heger et al, 2005(Heger et al, , 2006Heger and Klan, 2007;Krausková et al, 2016) can even lead to their crystallization under certain conditions.…”

Section: Introductionmentioning

confidence: 99%

Evaporating brine from frost flowers with electron microscopy and implications for atmospheric chemistry and sea-salt aerosol formation

Yang

Neděla²,

Runštuk³

et al. 2017

Atmos. Chem. Phys.

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Abstract. An environmental scanning electron microscope (ESEM) was used for the first time to obtain well-resolved images, in both temporal and spatial dimensions, of labprepared frost flowers (FFs) under evaporation within the chamber temperature range from −5 to −18 • C and pressures above 500 Pa. Our scanning shows temperature-dependent NaCl speciation: the brine covering the ice was observed at all conditions, whereas the NaCl crystals were formed at temperatures below −10 • C as the brine oversaturation was achieved. Finger-like ice structures covered by the brine, with a diameter of several micrometres and length of tens to 100 µm, are exposed to the ambient air. The brine-covered fingers are highly flexible and cohesive. The exposure of the liquid brine on the micrometric fingers indicates a significant increase in the brine surface area compared to that of the flat ice surface at high temperatures; the NaCl crystals formed can become sites of heterogeneous reactivity at lower temperatures. There is no evidence that, without external forces, salty FFs could automatically fall apart to create a number of sub-particles at the scale of micrometres as the exposed brine fingers seem cohesive and hard to break in the middle. The fingers tend to combine together to form large spheres and then join back to the mother body, eventually forming a large chunk of salt after complete dehydration. The present microscopic observation rationalizes several previously unexplained observations, namely, that FFs are not a direct source of sea-salt aerosols and that saline ice crystals under evaporation could accelerate the heterogeneous reactions of bromine liberation.

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“…By contrast, the dynamics of freezing forces the solutes to segregate and form the veins of freeze concentrated solutions engulfed by the ice (Blackford, 2007;Cheng et al, 2010;McCarthy et al, 2013;Bogdan et al, 2014;Krausko et al, 2014 ). The solutes in the freezeconcentrated solution, here probably in the surface layer only, experience, besides an increased concentration (Heger et al, 2005;Kania et al, 2014;Krausko et al, 2015a), also a changed pH (Heger et al, 2006;Krausková et al, 2016) and polarity (Heger and Klan, 2007). Recently, it was noticed that, for a frozen solution, the surface brine layer is interconnected with the interior veins system (Walker et al, 2013).…”

Section: Ffs At a High Temperature: Brine Fingers Formationmentioning

confidence: 99%

“…Such a model is generally unsatisfactory in describing the real situation, and thus more realistic parameters for modelling are needed. Some of us previously showed that the concentration increase of nonpolar (Heger et al, 2011;Kania et al, 2014;Krausko et al, 2015a;Krausko et al, 2015b ) and polar compounds (Heger et al, 2005;Heger et al, 2006;Heger and Klan, 2007;Krausková et al, 2016) can even lead to their crystallization under certain conditions.…”

Section: Introductionmentioning

confidence: 99%

Evaporating brine from frost flowers with electron microscopy, and implications for atmospheric chemistry and sea-salt aerosol formation

Yang¹,

Neděla²,

Runštuk³

et al. 2017

Preprint

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<p><strong>Abstract.</strong> An environmental scanning electron microscope was used for the first time to obtain well-resolved images, in both temporal and spatial dimensions, of lab-prepared frost flowers (FFs) under evaporation within the chamber temperature range from &#8722;5&#8201;&#176;C to &#8722;18&#8201;&#176;C and pressures above 500&#8201;Pa. Our scanning shows temperature-dependent NaCl speciation: the brine covering the ice was observed at all conditions, whereas the NaCl crystals were formed at temperatures below &#8722;10&#8201;&#176;C as the brine oversaturation was achieved. Finger-like ice structures covered by the brine, with a diameter of several micrometres and length of tens to one hundred micrometres, are exposed to the ambient air. The brine-covered fingers are highly flexible and cohesive. The exposure of the liquid brine on the micrometric fingers indicates a significant increase in the brine surface area compared to that of the flat ice surface at high temperatures, whereas the NaCl crystals can become sites of heterogeneous reactivity at lower temperatures. There is no evidence that, without external forces, salty FFs could automatically fall apart to create a number of sub-particles at the scale of micrometres as the exposed brine fingers seem cohesive and hard to break in the middle. The fingers tend to combine together to form large spheres and then join back to the mother body, eventually forming a large chunk of salt after complete dehydration. A present microscopic observation rationalizes several previously unexplained observations, namely, that FFs are not a direct source of sea salt aerosols and that saline ice crystals under evaporation could accelerate the heterogeneous reactions of bromine liberation.</p>

show abstract

Suppression of protein inactivation during freezing by minimizing pH changes using ionic cryoprotectants

Cited by 52 publications

References 70 publications

Cryopreservation and its clinical applications

Cryopreservation and its clinical applications

Evaporating brine from frost flowers with electron microscopy and implications for atmospheric chemistry and sea-salt aerosol formation

Evaporating brine from frost flowers with electron microscopy, and implications for atmospheric chemistry and sea-salt aerosol formation

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