Suppression of ROS generation by 4,4'-diaminodiphenylsulfone in non-phagocytic human diploid fibroblasts

Cho, Sung Chun; Rhim, Jong Kook; Son, Young Hoon; Lee, Suk Jin; Park, Sang Chul

doi:10.3858/emm.2010.42.3.024

Cited by 18 publications

(15 citation statements)

References 36 publications

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“…The DDS-treated worms exhibited a decreased ROS-induction response to PQ treatment and were less sensitive to PQ (Cho et al, 2010b). In the same study, we confirmed that DDS has an antioxidant effect in PQ-treated human dermal fibroblasts, in which it effectively suppresses ROS generation by inhibiting the NOX system (Cho et al, 2010a).…”

Section: Discussionsupporting

confidence: 75%

“…Previously, we reported that DDS could control PKCβ activity in human diploid fibroblasts (Cho et al, 2010a). In addition, our present study showed that DDS could effectively inhibit PQ-induced PKCμ activation in lung tissues and lung fibroblasts (Figures 5 and 6).…”

Section: Discussionmentioning

confidence: 48%

“…Many known antioxidant compounds exert their effects by scavenging ROS. However, we previously reported that DDS would not directly scavenge DPPH radicals in vitro, suggesting that DDS might exert its effects through regulation of ROS generation (Cho et al, 2010a). Paraquat induces superoxide anion production via NADPH-dependent metabolic pathways.…”

Section: Discussionmentioning

confidence: 97%

“…Previously, DDS has been known to suppress ROS production in phagocytic neutrophils (Suda et al, 2005). In addition, we also showed that DDS effectively suppresses ROS generation in non-phagocytic cells by inhibiting the NADPH oxidase (NOX) system (Cho et al, 2010a). Furthermore, we could recently report that DDS treatment significantly extends the lifespan of the nematode C. elegans (Cho et al, 2010b).…”

Section: Introductionmentioning

confidence: 94%

“…Therefore, the ability of DDS to reduce oxidative stress and thereby protect against PQ-induced mouse lung damage has been tested. As PQ treatment results in increased expression of NOX (Rossary et al, 2007;Cho et al, 2010a), which generates superoxide anions, we examined the expression of NOX2 and NOX4 mRNA in the lung tissues. The expression of NOX4 mRNA was Figure 5.…”

Section: Dds Controls the Pq-induced Mrna Expression Of Nox4mentioning

confidence: 99%

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Protective effect of 4,4'-diaminodiphenylsulfone against paraquat-induced mouse lung injury

et al. 2011

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Although 4,4'-diaminodiphenylsulfone (DDS, dapsone) has been used to treat several dermatologic conditions, including Hansen disease, for the past several decades, its mode of action has remained a topic of debate. We recently reported that DDS treatment significantly extends the lifespan of the nematode C. elegans by decreasing the generation of reactive oxygen species. Additionally, in in vitro experiments using non-phagocytic human fibroblasts, we found that DDS effectively counteracted the toxicity of paraquat (PQ). In the present study, we extended our work to test the protective effect of DDS against PQ in vivo using a mouse lung injury model. Oral administration of DDS to mice significantly attenuated the lung tissue damage caused by subsequent administration of PQ.Moreover, DDS reduced the local expression of mRNA transcripts encoding inflammation-related molecules, including endothelin-1 (ET-1), macrophage inflammatory protein-1α (MIP-1α), and transforming growth factor-β (TGF-β). In addition, DDS decreased the PQ-induced expression of NADPH oxidase mRNA and activation of protein kinase Cμ (PKCμ). DDS treatment also decreased the PQ-induced generation of superoxide anions in mouse lung fibroblasts. Taken together, these data suggest the novel efficacy of DDS as an effective protective agent against oxidative stress-induced tissue damages.

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Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 48%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 94%

Section: Dds Controls the Pq-induced Mrna Expression Of Nox4mentioning

confidence: 99%

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Protective effect of 4,4'-diaminodiphenylsulfone against paraquat-induced mouse lung injury

et al. 2011

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A comprehensive insight into the anti-inflammatory properties of dapsone

Khalilzadeh

Shayan

Jourian

et al. 2022

Naunyn-Schmiedeberg's Arch Pharmacol

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Dapsone in dermatology and beyond

2013

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Dapsone (4,4′-diaminodiphenylsulfone) is an aniline derivative belonging to the group of synthetic sulfones. In 1937 against the background of sulfonamide era the microbial activity of dapsone has been discovered. Shortly thereafter, the use of dapsone to treat non-pathogen-caused diseases revealed alternate antiinflammatory mechanisms that initially were elucidated by inflammatory animal models. Thus, dapsone clearly has dual functions of both: antimicrobial/antiprotozoal effects and anti-inflammatory features similarly to non-steroidal anti-inflammatory drugs. The latter capabilities primarily were used in treating chronic inflammatory disorders. Dapsone has been investigated predominantly by in vitro methods aiming to get more insights into the effect of dapsone to inflammatory effector cells, cytokines, and/or mediators, such as cellular toxic oxygen metabolism, myoloperoxidase-/halogenid system, adhesion molecules, chemotaxis, membrane-associated phospholipids, prostaglandins, leukotrienes, interleukin-8, tumor necrosis factor α, lymphocyte functions, and tumor growth. Moreover, attention has been paid to mechanisms by which dapsone mediates effects in more complex settings like impact of lifespan, stroke, glioblastoma, or as anticonvulsive agent. Additionally, there are some dermatological investigations in human being using dapsone and its metabolites (e.g., leukotriene B4-induced chemotaxis, ultraviolet-induced erythema). It could be established that dapsone metabolites by their own have anti-inflammatory properties. Pharmacology and mechanisms of action are determining factors for clinical use of dapsone chiefly in neutrophilic and/or eosinophilic dermatoses and in chronic disorders outside the field of dermatology. The steroid-sparing effect of dapsone is useful for numerous clinical entities. Future avenues of investigations will provide more information on this fascinating and essential agent.

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Suppression of ROS generation by 4,4'-diaminodiphenylsulfone in non-phagocytic human diploid fibroblasts

Cited by 18 publications

References 36 publications

Protective effect of 4,4'-diaminodiphenylsulfone against paraquat-induced mouse lung injury

Protective effect of 4,4'-diaminodiphenylsulfone against paraquat-induced mouse lung injury

A comprehensive insight into the anti-inflammatory properties of dapsone

Dapsone in dermatology and beyond

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