Suppression of Septoria tritici Blotch and Leaf Rust of Wheat by Recombinant Cyanide-Producing Strains of<i>Pseudomonas putida</i>

Flaishman, Moshe A.; Eyal, Z.; Zilberstein, Asher; Voisard, Christophe; Haas, Dieter

doi:10.1094/mpmi-9-0642

Cited by 119 publications

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“…However, there are no putative AlgR binding sites in or near the promoter Other studies have shown that P. aeruginosa produces HCN in infected burn eschar of human patients and that HCN was detectable in the viscera upon postmortem analysis (25). A more recent study has identified HCN produced by P. aeruginosa as a primary virulence factor for the paralytic killing of Caenorhabditis elegans by P. aeruginosa (23), and other studies have shown HCN to be an inhibitor of fungal growth in plant leaf and root infection (20,58). These studies indicate that the HCN produced by P. aeruginosa may affect host cells and may be important in virulence.…”

Section: Discussionmentioning

confidence: 99%

Identification of AlgR-Regulated Genes in Pseudomonas aeruginosa by Use of Microarray Analysis

et al. 2004

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The Pseudomonas aeruginosa transcriptional regulator AlgR controls a variety of different processes, including alginate production, type IV pilus function, and virulence, indicating that AlgR plays a pivotal role in the regulation of gene expression. In order to characterize the AlgR regulon, Pseudomonas Affymetrix GeneChips were used to generate the transcriptional profiles of (i) P. aeruginosa PAO1 versus its algR mutant in mid-logarithmic phase, (ii) P. aeruginosa PAO1 versus its algR mutant in stationary growth phase, and (iii) PAO1 versus PAO1 harboring an algR overexpression plasmid. Expression analysis revealed that, during mid-logarithmic growth, AlgR activated the expression of 58 genes while it repressed the expression of 37 others, while during stationary phase, it activated expression of 45 genes and repression of 14 genes. Confirmatory experiments were performed on two genes found to be AlgR repressed (hcnA and PA1557) and one AlgR-activated operon (fimU-pilVWXY1Y2). An S1 nuclease protection assay demonstrated that AlgR repressed both known hcnA promoters in PAO1. Additionally, direct measurement of hydrogen cyanide (HCN) production showed that P. aeruginosa PAO1 produced threefold-less HCN than did its algR deletion strain. AlgR also repressed transcription of two promoters of the uncharacterized open reading frame PA1557. Further, the twitching motility defect of an algR mutant was complemented by the fimTU-pilVWXY1Y2E operon, thus identifying the AlgR-controlled genes responsible for this defect in an algR mutant. This study identified four new roles for AlgR: (i) AlgR can repress gene transcription, (ii) AlgR activates the fimTU-pilVWXY1Y2E operon, (iii) AlgR regulates HCN production, and (iv) AlgR controls transcription of the putative cbb 3 -type cytochrome PA1557.

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Section: Discussionmentioning

confidence: 99%

Identification of AlgR-Regulated Genes in Pseudomonas aeruginosa by Use of Microarray Analysis

et al. 2004

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“…have been reported as cyanogenic strains (Flaishman et al 1996;Frey et al 2010). However, CN -was absent in our case.…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

Weathering of Granite from the Damma Glacier Area: The Contribution of Cyanogenic Bacteria

Wongfun

Plötze

Furrer

et al. 2013

Geomicrobiology Journal

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The dissolution potential of five cyanogenic bacteria was studied at 25°C during 32 days using granite material from the Damma glacier (Central Alps, Switzerland) as the sole source of nutrients. The bacterial species Pseudomonas fluorescens and Pseudomonas sp. CCOS 191 were the most effective to exudate various organic acids and consequently mobilised Fe. The molecular mechanisms include both, proton-promoted and ligand-promoted dissolution, preferentially at pH below 5 and in the pH range between 5.0 and 5.8, respectively. In addition, bacterially produced cyanide plays a minor role through the formation of soluble hexacyanoferrate complexes. To our knowledge, this study is the first that reveals the direct measurement of metal-cyanide complexes formed during biotic granite weathering.

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“…A leaf colonizing Pseudomonas sp. constructed to over produce HCN protected wheat from a leaf pathogen, Septocia tritici more effectively than the parent strain (Flaishman et al, 1996).…”

Section: Assay For Hydrogen Cyanide Productionmentioning

confidence: 99%

Isolation of <i>Pseudomonas flurescens</i> from Rhizosphere of Faba Bean and Screen Their Hydrogen Cyanide Production Under in Vitro Stduy, Ethiopia

Alemu¹

2016

AJLS

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Abstract:Hydrogen cyanide is a highly toxic volatile compound which interferes with the cellular respiration by inhibiting the cytochrome oxidase enzyme in mitochondria. Root area of crops were consisting several bacteria that may have beneficial aspects. The objective of this study was to investigate the qualitative capability of hydrogen cyanide production by Pseudomonas fluorescens species isolates. Isolation of Pseudomonas fluorescens isolates were carried out on King's B medium. Twelve Pseudomonas fluorescens species were isolated from the Rhizosphere of Fabaceae family namely faba bean screened for the production of hydrogen cyanide. For the production of hydrogen cyanide, Pseudomonas fluorescens isolates were streaked into King's B agar plates supplemented with glycine. Total of Pseudomonas fluorescens isolates had a potential to produce hydrogen cyanide. Therefore, these isolated can be used as a biological control against plant pathogen microbes.

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Suppression of Septoria tritici Blotch and Leaf Rust of Wheat by Recombinant Cyanide-Producing Strains ofPseudomonas putida

Cited by 119 publications

References 0 publications

Identification of AlgR-Regulated Genes in Pseudomonas aeruginosa by Use of Microarray Analysis

Identification of AlgR-Regulated Genes in Pseudomonas aeruginosa by Use of Microarray Analysis

Weathering of Granite from the Damma Glacier Area: The Contribution of Cyanogenic Bacteria

Isolation of <i>Pseudomonas flurescens</i> from Rhizosphere of Faba Bean and Screen Their Hydrogen Cyanide Production Under in Vitro Stduy, Ethiopia

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Suppression of Septoria tritici Blotch and Leaf Rust of Wheat by Recombinant Cyanide-Producing Strains ofPseudomonas putida

Cited by 119 publications

References 0 publications

Identification of AlgR-Regulated Genes in Pseudomonas aeruginosa by Use of Microarray Analysis

Identification of AlgR-Regulated Genes in Pseudomonas aeruginosa by Use of Microarray Analysis

Weathering of Granite from the Damma Glacier Area: The Contribution of Cyanogenic Bacteria

Isolation of &lt;i&gt;Pseudomonas flurescens&lt;/i&gt; from Rhizosphere of Faba Bean and Screen Their Hydrogen Cyanide Production Under in Vitro Stduy, Ethiopia

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Isolation of <i>Pseudomonas flurescens</i> from Rhizosphere of Faba Bean and Screen Their Hydrogen Cyanide Production Under in Vitro Stduy, Ethiopia