Suppression of serum IgE response and systemic anaphylaxis in a food allergy model by orally administered high-dose TGF-β

Okamoto, Atsushi; Kawamura, Tatsuyoshi; Kanbe, Kaori; Kanamaru, Yutaka; Ogawa, Hideoki; Okumura, Ko; Nakao, Akihiro

doi:10.1093/intimm/dxh250

Cited by 38 publications

(38 citation statements)

References 28 publications

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“…It has been reported that addition of recombinant TGFβ1 to spleen cell cultures stimulated with D. pteronyssinus inhibits IgE secretion [53]. Also oral administration of high-dose TGFβ1 simultaneously with ovalbumin significantly inhibits the elevation of ovalbumin-specific IgE levels and anaphylactic reactions in mice [54]. This inhibitory effect seems to be antigen-specific [55] and dependent on the TGFβ1 signaling pathway, since Smad3-null mice display higher levels of ovalbumin-specific IgE than wild-type mice [56].…”

Section: Discussionmentioning

confidence: 99%

The C-509T Promoter Polymorphism of the Transforming Growth Factor Beta-1 Gene Is Associated with Levels of Total and Specific IgE in a Colombian Population

Acevedo

Vergara

Gusmão

et al. 2009

Int Arch Allergy Immunol

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Background: The C-509T polymorphism of the transforming growth factor beta-1 (TGFB1) gene has been associated with asthma and asthma-related phenotypes, but its influence on total and specific IgE levels is controversial. Objective: To investigate the association between C-509T and asthma, as well as total IgE and specific IgE against Blomia tropicalis and Dermatophagoides pteronyssinus, in a Colombian population. Methods: The study population consisted of 417 asthmatics, 390 controls and 116 nuclear families. The C-509T polymorphism was genotyped using single-base extension minisequencing or Taq Man probes. IgE and TGFβ1 levels were measured by ELISA. Regression analysis and family-based association tests were performed in cases/controls and families. Associations were corrected by population structure using a panel of 52 ancestry informative markers in the case-control dataset. Results: There was no association between C-509T and asthma. In asthmatics, the CC genotype was associated with higher total IgE levels compared with the other genotypes [mean IgE: 2.81 ± 0.42 vs. 2.71 ± 0.45 log IU/ml; p = 0.016, corrected p value (p_c) = 0.019]. When only atopic asthmatics were included, the significance remained (p = 0.02, p_c = 0.03). In the family-based analyses, the C allele was associated with higher total IgE levels (p = 0.02) and the CC genotype with specific IgE to D. pteronyssinus (p = 0.01). Conclusions: C-509T is associated with total IgE levels and specific IgE to D. pteronyssinus in asthmatic patients. In contrast to other studies, we found the CC genotype to be associated with higher levels of total and specific IgE. Differences in the frequency of this allele among populations could alter its effects as a risk factor for asthma-associated phenotypes.

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Section: Discussionmentioning

confidence: 99%

The C-509T Promoter Polymorphism of the Transforming Growth Factor Beta-1 Gene Is Associated with Levels of Total and Specific IgE in a Colombian Population

Acevedo

Vergara

Gusmão

et al. 2009

Int Arch Allergy Immunol

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“…Murine mast cells deficient in Smad3, which is necessary for TGFb signaling, showed enhanced TNF and IL-6 responses to LPS. In vivo, W/Wv mice reconstituted with Smad3 -/-mast cells produced more proinflammatory cytokines (IL-6 and TNF) and recruited more neutrophils in the cecal ligation and puncture model of bacterial peritonitis, than controls [71,72]. TGFb produced by mast cells in response to bacterial challenge might also heighten the threshold for activation through IgE [73].…”

Section: Toll-like Receptor and Co-receptor Mediated Activation Of Mamentioning

confidence: 99%

New and emerging roles for mast cells in host defence

Dawicki

Marshall

2007

Current Opinion in Immunology

250

226

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“…We have previously demonstrated an important role for the maternal milk cytokine TGF-␤ in regulating the mucosal immune response after formula feeding in nonallergy-prone rat pups (9). High-dose TGF-␤ has also recently been shown to suppress IgE responses to food antigens in adult mice (17).…”

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confidence: 99%

Effects of Transforming Growth Factor-Beta and Formula Feeding on Systemic Immune Responses to Dietary β-Lactoglobulin in Allergy-Prone Rats

Penttila

2006

Pediatr Res

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Early nutritional events have the potential to affect health outcomes in later life including the development of allergy. Food allergy is usually the first manifestation of allergy. Breastfeeding has been associated with a protective effect against the development of allergy, but the evidence is contradictory and the mechanisms involved are not clear. We hypothesize that milk cytokines, such as transforming growth factor ␤ (TGF-␤), play a role in regulating immune responses to dietary antigens. Using a rat pup model of gastrostomy feeding, the immune response profile, at weaning and post-weaning, of allergy-prone Brown Norway rats fed formula supplementation with TGF-␤ was assessed. We show that feeding formula to allergy-prone rat pups results in increased total IgE immunoglobulin, ␤-lactoglobulin (BLG) IgG1 antibody, and mucosal mast cell activation, as measured by serum rat mast cell protease II (RMCPII) levels in the gut. Supplementation of formula with physiological levels of TGF-␤ down-regulated the BLG IgG1 response as well as total IgE and mucosal mast cell activation. Supplementation of formula also resulted in an increase in Th1 cytokines, interleukin (IL)-18, IL-12p40, IL-12p35, and interferon gamma (IFN-␥) and an increase in IL-10. In conclusion, TGF-␤ supplementation of formula moved the immune response profile of allergy prone (Th2 type) rat pups toward a Th1 profile in the suckling period. Importantly, this immune profile persisted after weaning when TGF-␤ was no longer present in the diet. (Pediatr Res 59: 650-655, 2006) E arly nutritional events have the potential to effect health outcomes in later life (1,2) including allergy development. Allergic disease arises due to complex interactions between genetic predisposition and environment including early nutritional events (3). Hereditary factors are important in the development of allergy as infants who go onto develop allergic disease show an early predisposition to Th2 responses to allergens in the first year of life. These responses are short-lived in infants without a predisposition to allergy (4). The mechanism involved in allergy development is at present unclear, although the ability to mount a Th1 immune response is important in preventing persistent Th2 immune responses and development of allergic disease. An imbalance favoring Th2 type immune responses increases the risk of allergic disease.Food allergy is usually the first manifestation of allergy with susceptible children going on to develop inhalant allergies as well (5). While the incidence of inhalant allergy is increasing, the incidence of food allergy is relatively stable (6). Breast-feeding has been associated with a protective effect against the development of allergy, but the evidence is contradictory and there is debate as to whether breast milk prevents or reduces the incidence of allergic disease (7,8). Van Odijk et al. (6) have carried out a multidisciplinary review of the literature on early feeding and impact on later allergy and concluded that breast-feeding...

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Suppression of serum IgE response and systemic anaphylaxis in a food allergy model by orally administered high-dose TGF-β

Cited by 38 publications

References 28 publications

The C-509T Promoter Polymorphism of the Transforming Growth Factor Beta-1 Gene Is Associated with Levels of Total and Specific IgE in a Colombian Population

The C-509T Promoter Polymorphism of the Transforming Growth Factor Beta-1 Gene Is Associated with Levels of Total and Specific IgE in a Colombian Population

New and emerging roles for mast cells in host defence

Effects of Transforming Growth Factor-Beta and Formula Feeding on Systemic Immune Responses to Dietary β-Lactoglobulin in Allergy-Prone Rats

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