Suppression of the interleukin-1β-induced inflammatory response of human Chang liver cells by acute and subacute exposure to alcohol: an in vitro study

Mörs, Katharina; Kany, Shinwan; Hörauf, Jason-Alexander; Wagner, Nils; Neunaber, Claudia; Perl, Mario; Marzi, Ingo; Relja, Borna

doi:10.3325/cmj.2018.59.46

Cited by 3 publications

(3 citation statements)

References 51 publications

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“…Interestingly, the ROS-reducing effects of alcohol were observed also after 24 h in monocytes, demonstrating prolonged suppressive influence of an acute intoxication with alcohol. The alterations regarding the ROS production during the observational timeline may be in line with findings reported by others demonstrating that the ROS production in human Chang liver cells depends on concentrations of alcohol to which the cells were exposed [28].…”

Section: Discussionsupporting

confidence: 90%

Reduced phagocytosis, ROS production and enhanced apoptosis of leukocytes upon alcohol drinking in healthy volunteers

Haag

Janicova

et al. 2021

Eur J Trauma Emerg Surg

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Background Alcohol drinking is associated with a serious risk of developing health problems as well as with a large number of traumatic injuries. Although chronic alcohol misuse is known to contribute to severe inflammatory complications, the effects of an acute alcohol misuse are still unclear. Here, the impact of acute alcohol drinking on leukocyte counts and their cellular functions were studied. Methods Twenty-two healthy volunteers (12 female, 10 male) received a predefined amount of a whiskey-cola mixed drink (40% v/v), at intervals of 20 min, over 4 h to achieve a blood alcohol concentration of 1‰. Blood samples were taken before drinking T0, 2 h (T2), 4 h (T4), 6 h (T6), 24 h (T24) and 48 h (T48) after starting drinking alcohol. Leukocytes, monocytes and granulocyte counts and their functions regarding the production of reactive oxidative species (ROS), phagocytosis and apoptosis were analyzed by flow cytometry. Results Total leukocyte counts significantly increased at T2 and T4, while granulocyte and monocyte counts decreased at T4 and T6 vs. T0. Monocytes increased significantly at T24 and T48 vs. T0. While the total number of ROS-producing leukocytes and notably granulocytes significantly increased, in parallel, the intracellular ROS intensity decreased at T2 and T6. The numbers of ROS-positive monocytes have shown a delayed modulation of ROS, with a significant reduction in the total number of ROS-producing cells at T48 and a significantly reduced intracellular ROS-intensity at T24. Phagocyting capacity of leukocytes significantly decreased at T4 and T6. In general leukocytes, and notably granulocytes demonstrated significantly increased early (T2), while monocyte exerted significantly increased late apoptosis (T24 and T48). Conclusions Alcohol drinking immediately impacts leukocyte functions, while the impact on monocytes occurs at even later time points. Thus, even in young healthy subjects, alcohol drinking induces immunological changes that are associated with diminished functions of innate immune cells that persist for days.

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Section: Discussionsupporting

confidence: 90%

Reduced phagocytosis, ROS production and enhanced apoptosis of leukocytes upon alcohol drinking in healthy volunteers

Haag

Janicova

et al. 2021

Eur J Trauma Emerg Surg

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“…Inflammasome components are present in multiple cell types, including HepG2 cells (Figure 3) [45,46]. The rationale for using HepG2, which is most commonly used in drug metabolism and hepatotoxicity studies [47], was determined by the fact that our and others' previous studies have already revealed the significant anti-inflammatory effects of acute alcohol administration upon the induction of inflammatory processes in various cell entities, including lung epithelial cells [28][29][30]48], human endothelial cells [49], macrophages and others [38,50]. Though the main mechanisms underlying the anti-inflammatory potential of acute ethanol intoxication under inflammatory conditions were linked to NF-κB [17], the impact of acute ethanol application on inflammatory processes specifically in terms of inflammasome functionality is mechanistically poorly investigated in hepatic cells.…”

Section: Discussionmentioning

confidence: 99%

Short Exposure to Ethanol Diminishes Caspase-1 and ASC Activation in Human HepG2 Cells In Vitro

Hörauf

Kany

Janicova

et al. 2020

IJMS

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This paper discusses how the assembly of pro-caspase-1 and apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC) in macromolecular protein complexes, inflammasomes, activates caspase-1. The present study investigates the molecular mechanisms of inflammasome activation in HepG2 cells and examines how short exposures to ethanol (EtOH) affect inflammasome activation. HepG2 cells were treated with lipopolysaccharide (LPS), ATP or nigericin (NIG) in a two-step model. After LPS priming, ATP or NIG were added. As inhibitors, sodium orthovanadate (general inhibitor of tyrosine phosphatases), AC-YVAD-CMK (caspase-1 inhibitor) or AZ10606120 (purinergic receptor P2X7R inhibitor) were applied after LPS priming. To monitor the inflammasome activation, the caspase-1 activity, ASC speck formation, reactive oxygen species (ROS) production and cell death were analyzed. To elucidate the mechanistical approach of EtOH to the inflammasome assembly, the cells were treated with EtOH either under simultaneous LPS administration or concurrently with ATP or NIG application. The co-stimulation with LPS and ATP induced a significant ASC speck formation, caspase-1 activation, cell death and ROS generation. The inhibition of the ATP-dependent purinoreceptor P2X7 decreased the caspase-1 activation, whereas sodium orthovanadate significantly induced caspase-1. Additional treatment with EtOH reversed the LPS and ATP-induced caspase-1 activation, ASC speck formation and ROS production. The ASC speck formation and caspase-1 induction require a two-step signaling with LPS and ATP in HepG2 cells. Inflammasome activation may depend on P2X7. The molecular pathway of an acute effect of EtOH on inflammasomes may involve a reduction in ROS generation, which in turn may increase the activity of tyrosine phosphatases.

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“…Enzyme Linked Immunosorbent Assay (ELISA) technique was used to measure, serum levels of Interlukine-6 (IL-6 ELISA Kit Dublin, Ireland), in diabetic patients with & without complication diabetes type2 and control group (18,19) Fasting Blood Glucose (FBG): Glucose was determined after enzymatic oxidation in the presence of glucose oxidase . The hydrogen peroxide formed reacts under catalysis of peroxidase, with phenol and 4-aminophenazone to form a red-violet quinoneimine dye as indicator (20) .…”

Section: (Il-6)mentioning

confidence: 99%

Relations between Interlukin-6 and Some Biochemical Parameters in Diabetic Foot Syndrome

2021

MLU

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The aim of this study is to predict the incidence of diabetic foot syndrome in type 2 diabetic patients by measuring some parameters (IL-6, FBS, MDA, CP, BMI). Samples were obtained from (75) type II diabetic patients with and without diabetic complications (diabetic foot and diabetic neuropathy), as well as ( 25) healthy controls as a control group. They were divided into four groups: Group A (the control group):-It included ( 25) healthy individuals aged between (35-70 years). Group B (Diabetics type II without complications):-Includes (25) patients, their ages range (35-70 years). Group C (diabetic patients with diabetic neuropathy):-It included (25) patients whose ages ranged from (35-70 years). Group D (diabetic patients with diabetic foot syndrome): -Included ( 25) patients whose ages ranged from (35-70 years). The results showed a significant increase in the levels of (IL-6, FBS, MDA, CP) in all groups of type II diabetes patients with and without complications compared to the control group (P≤ 0.05). There was a significant increase in the parameters measured in the group of diabetic patients with type II diabetic foot syndrome (DF) compared to the group of diabetic patients with type II disease Diabetic neuropathy (DN), and the results also showed a significant increase in group DN compared to group of type II diabetic patients (DM) (P≤ 0.05). Our study show Interleukin-6 values, as well as oxidative stress, could predict the future incidence of diabetic foot syndrome.

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Suppression of the interleukin-1β-induced inflammatory response of human Chang liver cells by acute and subacute exposure to alcohol: an in vitro study

Cited by 3 publications

References 51 publications

Reduced phagocytosis, ROS production and enhanced apoptosis of leukocytes upon alcohol drinking in healthy volunteers

Reduced phagocytosis, ROS production and enhanced apoptosis of leukocytes upon alcohol drinking in healthy volunteers

Short Exposure to Ethanol Diminishes Caspase-1 and ASC Activation in Human HepG2 Cells In Vitro

Relations between Interlukin-6 and Some Biochemical Parameters in Diabetic Foot Syndrome

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