Suppression of the ubiquitin E2 gene through RNA interference causes mortality in the banana weevil,<i>Cosmopolites sordidus</i>(Germar)

Ocimati, W.; Kiggundu, Andrew; Bailey, Ana M.; Niblett, C. L.; Pedun, H.; Tazuba, Anthony Fredrick; Tushemereirwe, Wilberforce; Karamura, E.

doi:10.17660/actahortic.2016.1114.25

Cited by 4 publications

(5 citation statements)

References 24 publications

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“…In vitro Actin-4 dsRNA treatment on R. similis impaired motility and reduced nematode multiplication on carrot discs (Roderick et al 2018). In vitro feeding of ubiquitin E2 gene dsRNA to banana weevil larva significantly arrested banana weevil larval growth and caused up to 100% mortality at 21 days (Ocimati et al 2016). Transgenic banana plants expressing siRNA or hairpin RNA of the above validated genes could, therefore, potentially be used for controlling the banana nematode and weevil infestation (Fig.…”

Section: Rnai Against Banana Diseasesmentioning

confidence: 99%

RNAi-mediated protection against banana diseases and pests

Ghag

Ganapathi

2019

3 Biotech

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Pests and pathogens restrict the production potential of many crop plants. The losses incurred due to pests and diseases are huge threatening food security. Management strategies include use of chemical pesticides which can be detrimental to human health and environment and other physical and biological methods which have serious limitations. An alternative would be to utilize the advanced technology such as RNA interference (RNAi) to engineer disease resistance in crop plants. The phenomenon of RNAi is very well studied in organisms across genera and found to be conserved. Taking advantage of this, dsRNAs have been delivered into pests and pathogens and showed significant growth inhibition. Banana is susceptible to various groups of pathogens which results in poor yield. The proof-of-principle studies using RNAi technology have already been demonstrated in banana to develop resistance to two important groups of pathogens. Transgenic banana plants expressing small interfering RNA targeting BBTV and Fusarium pathogen have shown high level of resistance. In this review, we summarize and discuss the studies utilizing RNAi as a strategy to develop resistance to major banana diseases and encourage further research in exploiting RNAi-based resistance in other crop plants.

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Section: Rnai Against Banana Diseasesmentioning

confidence: 99%

RNAi-mediated protection against banana diseases and pests

Ghag

Ganapathi

2019

3 Biotech

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“…Application of RNAi technology by designing dsRNA against the acetylcholinesterase (AChE) gene of banana aphids, showed a significant reduction in the aphid population (Jekayinoluwa et al 2021). RNAi-mediated suppression of the ubiquitin E2 gene caused 100% mortality in banana weevils (Ocimati et al 2014).…”

Section: Resistance To Insects and Nematodesmentioning

confidence: 99%

Host-delivered-RNAi-mediated resistance in bananas against biotic stresses

Das¹,

Nanda²

2022

J Exp Bio & Ag Sci

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Both the biotic and abiotic stressors restrict the yield potential of many crops, including bananas. Bananas belong to the genus Musa and are the world’s most popular and widely produced fruit for their nutritional and industrial importance. The demand for bananas is growing each day worldwide. However, different pest infestations are hampering the production of bananas, making it a matter of concern for global food security. Several biotechnological tools and applications including RNA interference (RNAi) have been employed to enhance the biotic stress resistance in plants. The capacity to silence targeted genes at transcriptional and post-transcriptional levels makes the RNAi technique a popular choice for gene knock-down and functional genomics studies in crops. Silencing of different suppressor molecule coding genes through RNAi helps crops to combat the detrimental effects of plant pathogens. The host-induced gene silencing (HIGS) technology, also known as the host-delivered RNAi (HD-RNAi), is nowadays gaining popularity due to its ability to target an array of pathogens, comprising bacteria, nematodes, fungi, viruses, and insects. This methodology is employed to manage disease pest outbreaks in a diverse range of crop species, including bananas. Besides HIGS, virus-induced and spray-induced gene silencing (VIGS and SIGS, respectively) are the potential approaches where RNAi technology is exploited to control plant-pathogenic diseases. The current review emphasizes the different kinds of diseases of bananas and the potential of HD-RNAi, a new-age and promising technology to build a barrier against significant crop and economic loss.

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“…In most of the oral RNAi studies retrieved in our search, the dsRNA was mixed with artificial diet, coated on leaves or presented through GM plant material. However, a study of the banana root borer Cosmopolites sordidus demonstrated that a topical application of 200-2000 ng of dsRNA targeting the ubiquitinE2 gene on the eggs also elicited successful gene silencing in hatching larvae, eventually causing 100% mortality 21 days after hatching (Ocimati et al, 2016).…”

Section: Dipteramentioning

confidence: 99%

Literature review of baseline information on RNAi to support the environmental risk assessment of RNAi‐based GM plants

Christiaens

Dzhambazova

Kostov

et al. 2018

EFS3

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This report is the outcome of an EFSA procurement (OC/EFSA/GMO/2015/02) which aims at reviewing relevant scientific information on RNA interference (RNAi) that could serve as baseline information for the environmental risk assessment of RNAi‐based genetically modified (GM) plants. The report is based on a systematic literature search on the use of RNAi molecules in arthropods, nematodes, annelids and molluscs with dsRNA, siRNA and miRNA applied primarily through feeding and soaking (oral ingestion). The numbers of retrieved publications covering these areas are reported, along with the species name, life stages tested, the target gene and its function, details of the test substances and their concentrations used, methods of delivery and effects. Separate sections discuss the available information on: (1) the uptake and systematic spread of RNAi activity, including a description of the various components involved in this process; (2) the mechanisms of dsRNA‐, siRNA‐ and miRNA‐elicited gene silencing and the different factors involved in RNAi efficiency; (3) routes of exposure of the biotic and abiotic environment to dsRNA, siRNA and miRNA from GM plants; (4) the environmental fate of dsRNA, siRNA and miRNA; and (5) the various factors that may limit non‐target effects including exposure, factors influencing the silencing efficiency of dsRNA, siRNA and miRNA, possible unintended and off‐target effects, and their mechanisms. Finally, an overview of the species of arthropods, nematodes, annelids and molluscs for which genomic data are available is also presented. The report identifies some of the challenges involved in developing plants with RNAi systems which affect invertebrate gene expression. The report also concludes that, currently, knowledge on issues such as exposure, specificity, offtarget effects, sequence similarities and bioinformatics is very limited, as only a few RNAi expressing plants which specifically target invertebrate species have been developed and comprehensively studied.

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Suppression of the ubiquitin E2 gene through RNA interference causes mortality in the banana weevil,Cosmopolites sordidus(Germar)

Cited by 4 publications

References 24 publications

RNAi-mediated protection against banana diseases and pests

RNAi-mediated protection against banana diseases and pests

Host-delivered-RNAi-mediated resistance in bananas against biotic stresses

Literature review of baseline information on RNAi to support the environmental risk assessment of RNAi‐based GM plants

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