Suppression of Transferrin Expression Enhances the Susceptibility of Plutella xylostella to Isaria cicadae

Abstract

Transferrins (Trfs) are multifunctional proteins with key functions in iron transport. In the present study, a Trf (PxTrf) from Plutella xylostella was identified and characterized. The PxTrf consisted of a 2046-bp open reading frame, which encoded a 681 amino acid protein with a molecular weight of 73.43 kDa and had an isoelectric point of 7.18. Only a single iron domain was predicted in the N-lobe of PxTrf. Although PxTrf was expressed ubiquitously, the highest levels of expression were observed in the fourth instar larvae. PxTrf transcript level was highest in fat bodies among various tissues. The PxTrf transcript levels increased significantly after the stimulation of pathogens. A decrease in PxTrf expression via RNA interference enhanced the susceptibility of P. xylostella to the Isaria cicadae fungus and inhibited hemocyte nodulation in response to the fungal challenge. In addition, a considerable increase in the pupation rate was observed in larvae treated with double-stranded PxTrf (dsPxTrf). Overall, according to the results, PxTrf may participate in P. xylostella immunity against fungal infection and insect development.

Iron regulatory pathways differentially expressed duringMadurella mycetomatisgrain development inGalleria mellonella

“…In our study, we did find expression of the ferritin subunit (LOC113510018), ferritin lower subunit (LOC113510017) and transferrin (LOC113509694) at all time points investigated. However, in contrast to what was seen in other fungal infections, expression levels did not significantly alter during infection (40). Transferrin and ferritin protein levels previously measured in the same G. mellonella infection model, on the other hand, were differentially abundant.…”

Silencing β‐1,3‐glucan binding protein enhances the susceptibility of Plutella xylostella to entomopathogenic fungus Isaria cicadae

“…The diamondback moth, Plutella xylostella , was reared as previously described 16 . Briefly, the larvae of P. xylostella were fed with bok choy ( Brassica rapa chinensis ) seedlings at 25 ± 1 °C, 75 ± 5% relative humidity and a 16 h:8 h (light:dark) photoperiod.…”

“…12,13 The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), a notorious pest of brassicaceae crops, has evolved serious resistance to various classes of insecticides and biopesticides. [14][15][16] Entomopathogenic fungi cause many insect diseases and have been developed as eco-friendly alternatives to chemical insecticides in pests biocontrol, especially for insecticide-resistant insects. 17 Therefore, insect immune response to fungal infection has attracted extensive attention.…”

Roles of CcDFR and CcOMT9 in the cyanidin biosynthesis and development of Cordyceps cicadae

“…Numerous studies have demonstrated its ability to enhance immune regulation activity, reduce inflammation, and inhibit tumor growth ( Layland et al, 2014 ; Wei et al, 2016 ; Meng et al, 2019 ; Nxumalo et al, 2020 ). In addition, several studies have demonstrated the potential of C. cicadae as a microbial insecticide against various insects, such as aphids ( Xu et al, 2020 ). Its natural composition is akin to that of Cordyceps sinensis , with active components primarily comprising polysaccharides, nucleosides, sterols, fatty acids and their derivatives, polyphenols, etc.…”