“…In rice cultivation, there is growing interest in achieving weed control with a more environment friendly method using organic matter, such as rice bran. The application of rice bran, a nutrient-rich by-product of the rice milling process [33], onto paddy fields was found to suppress the germination of Monochoria vaginalis, a noxious weed [34,35]. However, the precise mechanisms underlying its main herbicidal effects and impact on the soil microbial community are not fully understood.…”
Two Gram-stain-negative, terminal endospore-forming, rod-shaped and aerotolerant bacterial strains designated D1-1T and B3 were isolated from soil samples of an organic paddy in Japan. Strain D1-1T grew at 15–37 °C, pH 5.0–7.3, and with up to 0.5 % (w/v) NaCl. Phylogenetic analysis of the 16S rRNA gene revealed that strain D1-1T belonged to the genus
Clostridium
and was closely related to
Clostridium zeae
CSC2T (99.7 % sequence similarity),
Clostridium fungisolvens
TW1T (99.7 %) and
Clostridium manihotivorum
CT4T (99.3 %). Strains D1-1T and B3 were whole-genome sequenced and indistinguishable, with an average nucleotide identity value of 99.7 %. The average nucleotide identity (below 91.1 %) and digital DNA–DNA hybridization (below 43.6 %) values between the two novel isolates and their corresponding relatives showed that strains D1-1T and B3 could be readily distinguished from their closely related species. A novel
Clostridium
species, Clostridium folliculivorans sp. nov., with type strain D1-1T (=MAFF 212477T=DSM 113523T), is proposed based on genotypic and phenotypic data.
“…In rice cultivation, there is growing interest in achieving weed control with a more environment friendly method using organic matter, such as rice bran. The application of rice bran, a nutrient-rich by-product of the rice milling process [33], onto paddy fields was found to suppress the germination of Monochoria vaginalis, a noxious weed [34,35]. However, the precise mechanisms underlying its main herbicidal effects and impact on the soil microbial community are not fully understood.…”
Two Gram-stain-negative, terminal endospore-forming, rod-shaped and aerotolerant bacterial strains designated D1-1T and B3 were isolated from soil samples of an organic paddy in Japan. Strain D1-1T grew at 15–37 °C, pH 5.0–7.3, and with up to 0.5 % (w/v) NaCl. Phylogenetic analysis of the 16S rRNA gene revealed that strain D1-1T belonged to the genus
Clostridium
and was closely related to
Clostridium zeae
CSC2T (99.7 % sequence similarity),
Clostridium fungisolvens
TW1T (99.7 %) and
Clostridium manihotivorum
CT4T (99.3 %). Strains D1-1T and B3 were whole-genome sequenced and indistinguishable, with an average nucleotide identity value of 99.7 %. The average nucleotide identity (below 91.1 %) and digital DNA–DNA hybridization (below 43.6 %) values between the two novel isolates and their corresponding relatives showed that strains D1-1T and B3 could be readily distinguished from their closely related species. A novel
Clostridium
species, Clostridium folliculivorans sp. nov., with type strain D1-1T (=MAFF 212477T=DSM 113523T), is proposed based on genotypic and phenotypic data.
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