Suppressive effect of bacoside A on hypertrophic scar formation by downregulation of TGF-β1

Abstract

Purpose:To determine the anti-scarring property of bacoside A (BA) in a rabbit model of post-burns hypertrophic scar (HS). Methods: A total of 16 healthy male rabbits were divided into four groups (4 rabbits/group). The rabbits were subjected to full-thickness burn wound by placing a 10-mm brass rod at 90 ºC on their ears for 20 s, and were untreated (HS group). The remaining rabbits also underwent HS process but were topically treated daily with 0.2, 0.4 or 0.6 % BA gel (HS + 0.2, 0.4, and 0.6 % BA groups) from day 8 to day 34. Results: The mean values of collagen (types I & III), hydroxyproline (precursor of collagen) and hexosamine, as well as scar index significantly (p < 0.001) decreased after 28 days of topical treatment with BA. Moreover, BA application resulted in improved histological changes with proper arrangement of collagen fibers. Significant (p < 0.001) upregulation or downregulation of transforming growth factor beta-1 (TGF-β1) and matrix metalloproteinase-1 (MMP-1) were observed in the BA-treated rabbits. Conclusion: Treatment with BA significantly lowers the scarring area of burn wounds by downregulation of TGF-β1. Thus, BA can potentially be developed into a drug for managing injury scars. This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest

In Quest of the Mysterious Holistic Vedic Herb Bacopa monnieri (L.) Pennell

“…Therefore, in the development of pharmaceuticals for the suppression of scar formation, Bacoside-A has the potential for use. [336] Endocrine Effects Bacopa extract (200 mg/kg orally) has increased the thyroid hormone T 4 by 41% in mice, while T 3 has not been stimulated, implying that the extract is capable of directly stimulating the synthesis and/or release of T 4 at the glandular level without affecting the conversion of T 4 to T 3 . [337] Besides this, in hypothyroid and euthyroid animals, ethanolic extract of the whole plant increased both T 4 and T 3 and decreased TSH level, which revealed that Bacopa's thyrogenic activity is not only localized in the thyroid gland, but that certain central or peripheral actions may be taken by the herb.…”

Tetracyclic and Pentacyclic Triterpenes with High Therapeutic Efficiency in Wound Healing Approaches

“…Another in vivo study was performed in order to evaluate the effect of bacosides on the wound healing caused by burns in rabbits; after 28 days of topical application of a bacoside A extract, the levels of collagen, hydroxyproline and hexosamine decreased, as did the size of the wound. Authors hypothesized that the healing effect of bacoside A is due to a complex mechanism of action that involves: (i) changing the structure of collagen fibers; (ii) upregulation or downregulation of beta-1 growth factor and metalloproteinase-1 matrix transformation [112].…”

Dermal wound healing contribution of aqueous extracts ofAcalypha indica, Calotropis gigantea, Bacopa monnieriand their combination

“…Bacopa monnieri has been reported to moderate the secretion of collagen type I and III, improve collagen fibre organization and remodeling. 11,12 Acalypa indica was found to exhibit angiogenic activity by enhancing Vascular Endothelial Growth Factor (VEGF) and up-regulate selected pro-wound healing cytokines. [13][14][15] Topical application of Calotropis gigantea on wound was reported to modulate type I collagen secretion and reorganization during wound healing.…”