Suppressive Effects of Azithromycin on Zymosan-Induced Production of Proinflammatory Mediators by Human Corneal Epithelial Cells

Li, Dequan; Zhou, Nan; Zhang, Lili; Ma, Ping; Pflugfelder, Stephen C.

doi:10.1167/iovs.09-4992

Cited by 75 publications

(53 citation statements)

References 28 publications

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“…17 Azithromycin's anti-inflammatory properties have been shown to be mediated by its ability to inhibit pro-inflammatory NF-kB activity in lung carcinoma cell line 6 and to suppress zymosan-stimulated production of proinflammatory cytokines in human corneal epithelial cells. 18 These data support the hypothesis that ocular surface disease whereby an inflammatory cascade mediated by activation of the NFkB pathway, such as pterygium, may also be inhibited and relieved by topical azithromycin.…”

Section: Discussionsupporting

confidence: 79%

Topical Azithromycin 1% in acute management of inflammatory pterygium

et al. 2011

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The purpose of the article is to investigate the effects of topical azithromycin 1% (Azasite) in treating acute ocular inflammation related to long standing pterygium. A prospective interventional study of 8 consecutive cases with pterygium presenting to our University Eye Clinics over the period of one month with acute symptoms of red eye(s) and irritation. After patient education and receipt of consent to the study, patients were treated with topical azithromycin 1% (Azasite) twice a day for the first two days and once a day for the next 12 days. Anterior segment photo was taken before treatment and 3-day and/or 7-day after treatment in addition to eye examination.

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Section: Discussionsupporting

confidence: 79%

Topical Azithromycin 1% in acute management of inflammatory pterygium

et al. 2011

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“…Azithromycin inhibited production of inflammatory cytokines, chemokines and MMP-1, -3 and -9 in cultured human corneal epithelial cells (HCEC) stimulated by the toll like receptor 2 (TLR2) ligand, zymosan. 104 Azithromycin inhibited production of all of these MMPs in a dose-dependent fashion, showing greater reduction in MMP-9 production by zymosan-stimulated HCEC than anti-TLR2 antibody or an NF k B inhibitor. The effects of 4 weeks of topical azithromycin 1% therapy were evaluated on the mRNA expression of a variety of inflammatory mediators, including MMP-9, in conjunctival epithelial cells of patients with MGD.…”

Section: Azithromycinmentioning

confidence: 91%

Matrix metalloproteinase-9 in the pathophysiology and diagnosis of dry eye syndrome

Pflugfelder¹,

Bian²,

Paiva³

2017

MNM

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Dry eye and tear dysfunction are common ocular disorders that cause cornea barrier disruption resulting in a poorly lubricated and irregular cornea epithelium, eye irritation and blurred vision. Increased levels and activities of matrix metalloproteinases (MMPs), particularly MMP-9, have been detected in the tears and ocular surface epithelial and inflammatory cells in dry eye. MMPs have been found to participate in disruption of tight junctions in the apical cornea epithelium leading to their accelerated desquamation and barrier disruption. This review summarizes evidence showing the contribution of MMPs to dry eye pathogenesis and their roles as biomarkers and therapeutic targets. Keywords: matrix metalloproteinase, dry eye, keratitis sicca, cornea, barrier function, Sjögren syndrome Dry eye overviewDry eye and tear dysfunction are common ocular disorders that cause cornea barrier disruption resulting in a poorly lubricated and irregular cornea epithelium, eye irritation and blurred vision. It affects millions of people worldwide and is one of the most frequent conditions for which patients seek eye care.1 Aging and female sex are significant risk factors for dry eye which increases in prevalence around the fifth decade, with further increase every decade thereafter.2-13 Prevalence of dry eye varies from 2% to 50%, depending on the population studied and the diagnostic criteria for dry eye (symptom questionnaire vs. objective signs). [1][2][3]6,7,[9][10][11][14][15][16][17][18][19][20][21][22][23] There is scarce information about the natural history of dry eye, but there is a recognized disconnection between signs and symptoms; patients tend to be more symptomatic at early stages.24,25 Dry eye causes corneal irregularity [26][27][28] and decreases functional vision by altering contrast sensitivity [29][30][31] and, therefore, decreases quality of life with a significant burden on the individual as well as the society. 23,[32][33][34][35] A meta-analysis of 22 published studies showed increased odds ratio for depression and anxiety in patients with ocular Sjögren syndrome (SS). 36 There is increased evidence that dry eye is an inflammatory disease, and this review will focus on matrix metalloproteinases (MMPs) and their role in the pathogenesis of dry eye. Cornea barrier disruption is a feature of dry eyeAs the principal lens of the eye, the cornea has unique features to maintain its transparency and smooth surface that include a lack of blood vessels and a multilayered stratified, non-cornifying epithelium. The differentiated apical epithelial cells produce

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“…The cells were maintained in aMEM (Gibco, Carlsbad, CA) supplemented with 10% FBS, 100 U/ml penicillin and 100 mg/ml streptomycin. For experiments, MC3T3-E1 cells (passage number [10][11][12][13][14][15][16][17][18][19][20] were cultured retched to confluence, then the culture medium was supplemented with 50 mg/ml ascorbic acid and 10 mM b-glycerophosphate (Sigma, St. Louis, MO) to support differentiation (differential medium). 8 The cells were cultured for a further 4 days.…”

Section: Methodsmentioning

confidence: 99%

“…10 The positive control was cells incubated with HKLM but without the anti-TLR2 antibody, whereas the negative control lacked both HKLM and the anti-TLR2 antibody.…”

Section: Blocking Test With An Anti-tlr2 Antibodymentioning

confidence: 99%

Suppression of osteoblast toll‐like receptor 2 signaling by endothelin‐1

Suzaki

Komine‐Aizawa

Hayakawa

2014

Journal Orthopaedic Research

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Peripheral endothelin-1 (ET-1) levels are increased in chronic systemic disorders such as congestive cardiac failure, diabetes and chronic renal failure. Bone infections are also associated with poor prognoses in these conditions. In the present study, we examined the alterations in Toll-like receptor 2 (TLR2) signaling induced by ET-1 in an in vitro osteoblast cell model. The TLR2-positive murine osteoblast cell line MC3T3-E1 was treated with heat-killed Listeria monocytogenes (HKLM), a TLR2 ligand, in the presence or absence of ET-1. We examined TLR2 expression, intranuclear NF-kB phosphorylation and interleukin 6 (IL-6) production. ET-1 suppressed cell surface expression of TLR2, NF-kB phosphorylation and IL-6 production. As TLR2 represents an important mechanism by which osteoblasts recognize bacterial pathogens, a continuously elevated ET-1 status may impair pathogenic recognition by osteoblasts and consequently affect bone metabolism during infections.

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Suppressive Effects of Azithromycin on Zymosan-Induced Production of Proinflammatory Mediators by Human Corneal Epithelial Cells

Cited by 75 publications

References 28 publications

Topical Azithromycin 1% in acute management of inflammatory pterygium

Topical Azithromycin 1% in acute management of inflammatory pterygium

Matrix metalloproteinase-9 in the pathophysiology and diagnosis of dry eye syndrome

Suppression of osteoblast toll‐like receptor 2 signaling by endothelin‐1

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