Suppressive effects of &lt;i&gt;Pythium oligandrum&lt;/i&gt; on soybean damping off caused by &lt;i&gt;P. aphanidermatum&lt;/i&gt; and &lt;i&gt;P. myriotylum&lt;/i&gt;

You, Xiaodong; Barraud, Joffroy; Tojo, Motoaki

doi:10.4165/kapps.61.9

Cited by 11 publications

(6 citation statements)

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“…The mechanism of action of Pythium oligandrum as an active compound of Polyversum is not yet entirely clear and probably combines multiple action modes. The work of You et al [59] mentioned competition for space and nutrients as a probable mechanism; other possibilities include antibiosis and parasitism [60,61] and induced resistance [62]. Our results document the strong positive influence of this BCA on the growth of the plant in the absence of the pathogen.…”

Section: Discussionsupporting

confidence: 67%

A Comparison of the Ability of Some Commercially Produced Biological Control Agents to Protect Strawberry Plants against the Plant Pathogen Phytophthora cactorum

et al. 2021

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A comparison of the ability of commercially produced biological control agents—Contans, Gliorex, Hirundo, Polyversum, Prometheus, Clonoplus, Integral Pro and Xilon GR, completed with an isolate of Clonostachys rosea and of Pseudomonas sp.—to protect strawberry plants against Phytophthora cactorum was performed. The experiment was performed on strawberry cultivars Sonata, Karmen, and Wendy—cultivated in a cultivating room and greenhouse. The health of plants was affected negatively by the pathogen in all variants of biological agents used, but differences were seen in the rates of this decrease. The results revealed the ability of some tested agents to improve the growth of plants in the absence of the pathogen; the preparation Polyversum (Pythium oligandrum) was the most beneficial, in both the presence and absence of the pathogen. Contrarily, some agents alone decreased the health of plants; Integral Pro (Bacillus subtillis) and a strain of Pseudomonas sp. caused a deterioration in the health of the plants, even in the absence of a pathogen. The results of our analysis demonstrate the varied usefulness of all agents under unified environmental conditions; their effect seems to be dependent on the conditions and on the combination of the genotypes of all three participants in the interaction: plant–pathogen–antagonist.

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Section: Discussionsupporting

confidence: 67%

A Comparison of the Ability of Some Commercially Produced Biological Control Agents to Protect Strawberry Plants against the Plant Pathogen Phytophthora cactorum

et al. 2021

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“…It was speculated by Le (2016) that the lack of a control effect may have been in part due to poor colonization of the soil by the P. oligandrum isolate (vermiculite was used to grow the ginger plants inoculated with GAQ1) or the ineffective application of the oospore suspension by soil drenching (mycelia of the GAQ1 isolate was added directly into the vermiculite). However, there is support for P. oligandrum to control other diseases caused by P. myriotylum where previously it was shown that P. oligandrum was able to reduce soybean damping-off caused by P. myriotylum (You et al, 2019).…”

Section: P Oligandrum Gaq1 Has Control Effect On Pythium Soft-rot Of P Myriotylum Infected Gingermentioning

confidence: 99%

Dual-Transcriptomic, Microscopic, and Biocontrol Analyses of the Interaction Between the Bioeffector Pythium oligandrum and the Pythium Soft-Rot of Ginger Pathogen Pythium myriotylum

et al. 2021

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Biological control is a promising approach to suppress diseases caused by Pythium spp. such as Pythium soft rot of ginger caused by P. myriotylum. Unusually for a single genus, it also includes species that can antagonize Pythium plant pathogens, such as Pythium oligandrum. We investigated if a new isolate of P. oligandrum could antagonize P. myriotylum, what changes occurred in gene expression when P. oligandrum (antagonist) and P. myriotylum (host) interacted, and whether P. oligandrum could control soft-rot of ginger caused by P. myriotylum. An isolate of P. oligandrum, GAQ1, recovered from soil could antagonize P. myriotylum in a plate-based confrontation assay whereby P. myriotylum became non-viable. The loss of viability of P. myriotylum coupled with how P. oligandrum hyphae could coil around and penetrate the hyphae of P. myriotylum, indicated a predatory interaction. We investigated the transcriptional responses of P. myriotylum and P. oligandrum using dual-RNAseq at a stage in the confrontation where similar levels of total transcripts were measured from each species. As part of the transcriptional response of P. myriotylum to the presence of P. oligandrum, genes including a subset of putative Kazal-type protease inhibitors were strongly upregulated along with cellulases, elicitin-like proteins and genes involved in the repair of DNA double-strand breaks. In P. oligandrum, proteases, cellulases, and peroxidases featured prominently in the upregulated genes. The upregulation along with constitutive expression of P. oligandrum proteases appeared to be responded to by the upregulation of putative protease inhibitors from P. myriotylum, suggesting a P. myriotylum defensive strategy. Notwithstanding this P. myriotylum defensive strategy, P. oligandrum had a strong disease control effect on soft-rot of ginger caused by P. myriotylum. The newly isolated strain of P. oligandrum is a promising biocontrol agent for suppressing the soft-rot of ginger. The dual-RNAseq approach highlights responses of P. myriotylum that suggests features of a defensive strategy, and are perhaps another factor that may contribute to the variable success and durability of biological attempts to control diseases caused by Pythium spp.

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“…P. oligandrum isolates (MS15, MS19, MS31) parasitized hyphae of P. aphanidermatum by entangling the hyphae of P. aphanidermatum with thin haustorial branches or infection pegs, eventually leading to destruction, revealing the mode of action of antagonism between P. oligandrum and P. aphanidermatum [20,29] as appears in our results of screening for hyphal interaction under light and scanning microscopy. There were at least three known modes of P. oligandrum action on plant pathogens: mycoparasitism mediated by intimate hyphae interactions, antibiosis and enhancement of plant resistance due to protein metabolite (oligandrin) production [8].…”

Section: Discussionmentioning

confidence: 74%

“…These results were confirmed through the reduction of the mycelial dry weight of pathogenic P. aphanidermatum with half-strength millipore sterilized filtrates of the bioactive P. oligandrum MS15 to 40.11% compared with autoclaved filtrate (35.7%). That explains the low activity of autoclaved P. oligandrum filtrates, which may affect and destroy the active metabolites [28][29][30][31].…”

Section: Discussionmentioning

confidence: 99%

Myco-Suppression Analysis of Soybean (Glycine max) Damping-Off Caused by Pythium aphanidermatum

Sayed

Abdelmohsen

Abdelzaher

et al. 2021

Plants

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The role of Pythium oligandrum as a biocontrol agent against Pythium aphanidermatum was investigated to avoid the harmful impacts of fungicides. Three isolates of P. oligandrum (MS15, MS19, and MS31) were assessed facing the plant pathogenic P. aphanidermatum the causal agent of Glycine max damping-off. The tested Pythium species were recognized according to their cultural and microscopic characterizations. The identification was confirmed through sequencing of rDNA-ITS regions including the 5.8 S rDNA. The biocontrol agent, P. oligandrum, isolates decreased the mycelial growth of the pathogenic P. aphanidermatum with 71.3%, 67.1%, and 68.7% through mycoparasitism on CMA plates. While the half-strength millipore sterilized filtrates of P. oligandrum isolates degrade the pathogenic mycelial linear growth by 34.1%, 32.5%, and 31.7%, and reduce the mycelial dry weight of the pathogenic P. aphanidermatum by 40.1%, 37.4%, and 36.8%, respectively. Scanning electron microscopy (SEM) of the most effective antagonistic P. oligandrum isolate (MS15) interaction showed coiling, haustorial parts of P. oligandrum to P. aphanidermatum hyphae. Furthermore, P. oligandrum isolates were proven to enhance the germination of Glycine max seedling to 93.3% in damping-off infection using agar pots and promote germination of up to 80% during soil pot assay. On the other hand, P. oligandrum isolates increase the shoot, root lengths, and the number of lateral roots.

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Suppressive effects of <i>Pythium oligandrum</i> on soybean damping off caused by <i>P. aphanidermatum</i> and <i>P. myriotylum</i>

Cited by 11 publications

References 20 publications

A Comparison of the Ability of Some Commercially Produced Biological Control Agents to Protect Strawberry Plants against the Plant Pathogen Phytophthora cactorum

A Comparison of the Ability of Some Commercially Produced Biological Control Agents to Protect Strawberry Plants against the Plant Pathogen Phytophthora cactorum

Dual-Transcriptomic, Microscopic, and Biocontrol Analyses of the Interaction Between the Bioeffector Pythium oligandrum and the Pythium Soft-Rot of Ginger Pathogen Pythium myriotylum

Myco-Suppression Analysis of Soybean (Glycine max) Damping-Off Caused by Pythium aphanidermatum

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