Suppressive Influences of Methotrexate on the Generation of CD14 (+) Monocyte-Lineage Cells from Bone Marrow of Patients with Rheumatoid Arthritis

Peripheral blood gene expression profiling in rheumatoid arthritis

“…Methotrexate has been reported to suppress the spontaneous generation of cells that express CD14 from CD14-negative bone marrow progenitor cells in RA patients. 19 Therefore, it is unlikely that monocytosis is related to methotrexate treatment, and in any case, such an association was not observed. In addition, the 14 patients who were taking methotrexate at the time of blood draw did not all cluster together, indicating that methotrexate does not account for the difference seen between RA and control subjects.…”

Differential influences of bucillamine and methotrexate on the generation of fibroblast-like cells from bone marrow CD34+ cells of rheumatoid arthritis patients

“…Recently it has been proposed that DMARDs may work by inhibiting myelopoiesis by supplying fewer inflammatory cells in the inflamed joints [4]. In fact, we have shown that gold sodium thiomalate (GST) as well as MTX inhibits the generation of CD14 (+) cells (type A synoviocyte-like cells) from the bone marrow progenitor cells of RA patients confirming that bone marrow progenitor cells are one of the targets of DMARDs [15,16]. However, the influence of DMARDs, including MTX, on the generation of type B synoviocyte-like cells has not been delineated.…”

“…In addition, it was found that MTX at 20 nM suppressed the proliferation of human monocytic cells line U937 cells as well as the production of IL-1β by RA bone marrow mononuclear cells [29]. Taken together, these data suggest that MTX might inhibit the generation of type A synoviocytes and their function [16]. On the other hand, BUC-ID at 3μM did not suppress the generation of CD14+ cells from RA bone marrow CD14− cells, suggesting that BUC-ID might not affect the generation of type A synoviocytes and their function in RA [15].…”

Circulating Osteoclast Precursors: A Mechanism and a Marker of Erosive Arthritis

“…Subsequently, accelerated generation of CD14 + monocyte-lineage cells from the bone marrow of RA patients is observed [54]. Interestingly, the increased CD14 + cells can be reversed by treatment of patients with disease-modifying antirheumatic drug, Methotrexate [55]. Bone marrow stromal cells from RA patients fail to support normal hematopoiesis and produce abnormally high amounts of TNF.…”