Suppressive role of diallyl trisulfide in the activated platelet-mediated hematogenous metastasis of MDA-MB-231 human breast cancer cells

Abstract

Accumulating evidence has indicated that garlic consumption may reduce the risk of developing several types of cancer, and extensive studies have revealed the effects of its bioactive component, diallyl trisulfide (DATS), on the proliferation and apoptosis of tumor cells. The present study was undertaken to examine whether DATS affects hematogenous metastasis. In view of the dynamic crosstalk interplayed by tumor cells and platelets in hematogenous metastasis, we attempted to demonstrate the role of DATS in the metastatic behavior of MDA-MB-231 human breast cancer cells, which were co-incubated with activated platelets. Indeed, our data indicated that DATS significantly blocked platelet activation and aggregation induced by platelet-activating factor (PAF), and decreased the production of thromboxane B2 (TXB2). It was also found that DATS suppressed the migration and invasion of MDA-MB-231 cells in the presence of platelets activated by PAF in vitro in a dose-dependent manner. Furthermore, our results revealed thaat the release of activated TGF-β1 in the platelet-tumor cell system was markedly attenuated by DATS. Therefore, our findings strongly suggest that the diverse pharmacological activities of DATS are at least partially reflected by the interruption of the activated platelets-mediated metastasis of breast cancer cells.

Extracellular Microparticles Encapsulated with Diallyl Trisulfide Interfere with the Inflammatory Tumor Microenvironment and Lung Metastasis of Invasive Melanoma

“…6,7 It was also found that DATS suppressed metastasis by reducing the levels of a series of inflammatory factors, such as the tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), limiting the remodeling of the extracellular matrix in the tumor microenvironment (TME), and diminishing hypoxia-induced tumor angiogenesis. 8,9 Despite the fact that DATS possesses potent antimetastatic activity, there has been no efficient carrier to deliver it to tumor sites in vivo. An array of nanodrug delivery systems with targeting technologies have been used to create DATS formulations.…”

“…Sulfur-containing compounds such as diallyl trisulfide (DATS) isolated from garlic have attracted great attention for their antitumor effects. Previous studies demonstrated that DATS inhibited metastasis by decreasing the migration and invasion of tumor cells in various animal tumor models, such as breast cancer and melanoma models. , It was also found that DATS suppressed metastasis by reducing the levels of a series of inflammatory factors, such as the tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), limiting the remodeling of the extracellular matrix in the tumor microenvironment (TME), and diminishing hypoxia-induced tumor angiogenesis. , Despite the fact that DATS possesses potent antimetastatic activity, there has been no efficient carrier to deliver it to tumor sites in vivo . An array of nanodrug delivery systems with targeting technologies have been used to create DATS formulations.…”

Diallyl disulfide and diallyl trisulfide in garlic as novel therapeutic agents to overcome drug resistance in breast cancer

“…Liu et al. [ 92 ] found that DATS inhibits metastasis of BC cells by attenuating activated transforming growth factor-β1 signaling. Bauer et al.…”

The killing effect of Tanshinol on breast cancer cells: insight into the reversion of TGF-𝜷1-mediated suppression of NK cell functions

“…Our previous studies showed that the release of TGF-β1 was significantly increased in the activated platelet-tumor cell co-culture system, and repression of TGF-β1 could dramatically retard the malignant biological progression of breast cancer cells, suggesting that TGF-β1 may act as a key molecule mediating the interactions between tumor cells and platelets [8]. It has been documented that Salvia miltiorrhiza exerts an important effect on enhancing immunity, which was evidenced by the fact that it boosted the killing activities of cytotoxic T lymphocytes (CTL) and NK cells and stimulated the phagocytosis of macrophages.…”